Aspiration cytology of neuroblastoma: Light microscopy with transmission and scanning electron microscopic correlations

1988 ◽  
Vol 4 (4) ◽  
pp. 323-327 ◽  
Author(s):  
Mohammed Akhtar ◽  
Muhammad Ashraf Ali ◽  
Kwesi Sackey ◽  
Rajeh Sabbah ◽  
Mohammed Bakry
Keyword(s):  
Light Microscopy ◽  
Electron Microscopic ◽  
Aspiration Cytology ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

scholarly journals Scanning Electron Microscopic Study of Modified Chloroplasts in Senescing Broccoli Florets

HortScience ◽  
2000 ◽  
Vol 35 (1) ◽  
pp. 99-103 ◽  
Author(s):  
Hirofumi Terai ◽  
Alley E. Watada ◽  
Charles A. Murphy ◽  
William P. Wergin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Structural Changes ◽  
Freeze Fracture ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Scanning Electron Microscopic Study ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

Structural changes in chloroplasts of broccoli (Brassica oleracea L., Italica group) florets during senescence were examined using light microscopy, scanning electron microscopy (SEM) with freeze-fracture technique, and transmission electron microscopy (TEM) to better understand the process of chloroplast degradation, particularly at the advanced stage of senescence. Light microscopy revealed that chloroplasts, which initially were intact and green, became obscure in shape, and their color faded during senescence. Small, colored particles appeared in cells as the florets approached the final stage of senescence and became full- to dark-yellow in color. Scanning electron microscopy showed that stroma thylakoids in the chloroplast initially were parallel to each other and grana thylakoids were tightly stacked. As senescence advanced, the grana thylakoids degenerated and formed globules. The globules became larger by aggregation as senescence progressed, and the large globules, called “thylakoid plexus,” formed numerous vesicles. The vesicles ultimately were expelled into the cytosol, and the light microscope revealed many colored particles in the senescent cells. These results indicate that the degradation of chloroplasts in broccoli florets progresses systematically, with the final product being colored particles, which are visible in yellow broccoli sepal cells.

Light and scanning electron microscopic studies on Trichodina labrisomi n.sp. from Labrisomas nuchipinnis (Osteichthyes: Labrisomidae) from Mangrove Lake, Bermuda

1993 ◽  
Vol 71 (9) ◽  
pp. 1855-1860
Author(s):  
Thomas G. Rand
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Body Shape ◽  
The Body ◽  
Electron Microscopic ◽  
Scanning Electron Microscopic ◽  
Microscopic Studies ◽  
Oral Surface ◽  
Scanning Electron

Trichodina labrisomi n.sp. from the gills of the hairy blenny, Labrisomas nuchipinnis (Quoy and Gaimard), in Mangrove Lake, Bermuda, is described using light microscopy and scanning electron microscopy (SEM). Light microscopy reveals that the dimensions and morphology of T. labrisomi are similar to those given for only 13 previously described species of Trichodina (T. acuta Lom, 1961; T. baicalensis Dogiel, 1957; T. cottomephori Stein, 1979; T. dalli Zhukov, 1964; Trichodina domerguei domerguei Lom and Shtein 1966; T. elegans Stein, 1979; T. fultoni Davis, 1947; T. jadranica Haider, 1964; T. jadranica noblei Lom, 1970; T. partidisci Haider, 1964; T. reticulata Hirschmann et Partsch, 1955; T. tenuidens Faure-Fremiet, 1943; and T. tenuiformis Stein, 1979). However, compared with these similar forms, T. labrisomi n.sp. is distinguished by the dimensions of its body and denticulate ring and (or) the appearance of its silver-impregnated adhesive disc and denticles. Features of T. labrisomi examined by SEM include the body shape, pellicle, aboral pellicular pores, border membrane, aboral ciliary complex, and adoral ciliature, which are described and compared with those of other trichodinids studied using SEM. SEM also revealed that bacilli were distributed circumequatorially over the oral surface of T. labrisomi.

Apothecium morphogenesis in Peziza quelepidotia: scanning electron and light microscopy

1976 ◽  
Vol 54 (19) ◽  
pp. 2254-2267 ◽  
Author(s):  
K. L. O'Donnell ◽  
G. R. Hooper ◽  
W. G. Fields ◽  
Arthur O. Ackerson
Keyword(s):  
Light Microscopy ◽  
Unique Feature ◽  
Spatial Relationship ◽  
Scanning Electron Micrographs ◽  
Electron Microscopic ◽  
Functional Image ◽  
Scanning Electron Microscopic ◽  
Electron And Light Microscopy ◽  
Whole Mounts ◽  
Scanning Electron

This study on Peziza quelepidotia Korf & O'Donnell, a homothallic operculate Discomycete (Pezizales, Pezizaceae), presents the first detailed correlative light microscopic and scanning ultrastructural observations of any species in this genus. This investigation was possible because P. quelepidotia. unlike other Peziza spp., produces fertile ascomata in pure culture. A complete structural and functional image of apothecium morphogenesis was obtained by combining scanning electron micrographs of the initial stages with light micrographs of whole mounts and thick sections of apothecia embedded in plastic at all stages of development. The temporal–spatial relationship between the ascogonia and the Botrytis-like imperfect state suggests that the ascogonial coils may be under chemotrophic control of the conidiophores. Although functional ascogonia are present, sexuality is apparently parthenogenetic since antheridia were never observed. A unique feature of paragymnohymenial apothecium development in this species is the elevation of the ascogonial coil as the stipe develops. Scanning electron microscopic observations on longitudinally cryofractured ascocarps are presented.

Scanning Electron Microscopic Study of Adult Stored Product Beetle Elytra

1978 ◽  
Vol 61 (1) ◽  
pp. 76-87
Author(s):  
John E Kvenberg
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Electron Microscopic ◽  
Scanning Electron Microscopic Study ◽  
Beetle Elytra ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

Abstract Scanning electron microscopy was used to establish the microstructure of adult stored product beetle elytra. Twenty-three species of major importance, which commonly occur in food products, are illustrated. Micrographs and accompanying narrative descriptions provide a definitive diagnostic reference for the identification of elytral fragments examined by light microscopy.

Scanning Electron Microscopic Study of the Cell Surface

1969 ◽  
Vol 27 ◽  
pp. 36-37 ◽  
Author(s):  
Toichiro Kuwabara
Keyword(s):  
Tissue Fluid ◽  
Biological Application ◽  
Biological Structure ◽  
Electron Microscopic ◽  
Surface Appearance ◽  
Minute Amount ◽  
Scanning Electron Microscopic Study ◽  
Scanning Electron Microscopic ◽  
True Structure ◽  
Scanning Electron

Although scanning electron microscopy has a great potential in biological application, there are certain limitations in visualization of the biological structure. Satisfactory techniques to demonstrate natural surfaces of the tissue and the cell have been reported by several investigators. However, it is commonly found that the surface cell membrane is covered with a minute amount of mucin, secretory substance or tissue fluid as physiological, pathological or artefactual condition. These substances give a false surface appearance, especially when the tissue is fixed with strong fixatives. It seems important to remove these coating substances from the surface of the cell for demonstration of the true structure.

A scanning electron microscopic study on dissolving process of cholesterol gallstones by chenodeoxycholic acid (CDCA)

1978 ◽  
Vol 36 (2) ◽  
pp. 522-523
Author(s):  
T. Kanetaka ◽  
M. Cho ◽  
S. Kawamura ◽  
T. Sado ◽  
K. Hara
Keyword(s):  
Electron Microscope ◽  
Chenodeoxycholic Acid ◽  
Outer Surface ◽  
Electron Microscopic ◽  
Cholesterol Gallstones ◽  
The Core ◽  
Scanning Electron Microscopic Study ◽  
Scanning Electron Microscopic ◽  
Acceleration Voltage ◽  
Scanning Electron

The authors have investigated the dissolution process of human cholesterol gallstones using a scanning electron microscope(SEM). This study was carried out by comparing control gallstones incubated in beagle bile with gallstones obtained from patients who were treated with chenodeoxycholic acid(CDCA).The cholesterol gallstones for this study were obtained from 14 patients. Three control patients were treated without CDCA and eleven patients were treated with CDCA 300-600 mg/day for periods ranging from four to twenty five months. It was confirmed through chemical analysis that these gallstones contained more than 80% cholesterol in both the outer surface and the core.The specimen were obtained from the outer surface and the core of the gallstones. Each specimen was attached to alminum sheet and coated with carbon to 100Å thickness. The SEM observation was made by Hitachi S-550 with 20 kV acceleration voltage and with 60-20, 000X magnification.

A Comparative Study of Fractographic Replicas

1974 ◽  
Vol 32 ◽  
pp. 566-567
Author(s):  
Loren Anderson ◽  
Pat Pizzo ◽  
Glen Haydon
Keyword(s):  
Electron Microscopy ◽  
Electron Microscopic Examination ◽  
Direct Examination ◽  
Electron Microscopic ◽  
Reliable Technique ◽  
Service Failures ◽  
Transmission Electron ◽  
Mode Of Failure ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

Transmission electron microscopy of replicas has long been used to study the fracture surfaces of components which fail in service. Recently, the scanning electron microscope (SEM) has gained popularity because it allows direct examination of the fracture surface. However, the somewhat lower resolution of the SEM coupled with a restriction on the sample size has served to limit the use of this instrument in investigating in-service failures. It is the intent of this paper to show that scanning electron microscopic examination of conventional negative replicas can be a convenient and reliable technique for determining mode of failure.

A Scanning Electron Microscopic Study of the Uriniferous Tubules

1973 ◽  
Vol 31 ◽  
pp. 622-623
Author(s):  
Peter M. Andrews
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Renal Glomerulus ◽  
Electron Microscopic ◽  
Vascular Perfusion ◽  
Scanning Electron Microscopic Study ◽  
Scanning Electron Microscopic ◽  
Collecting Tubules ◽  
Bowman's Capsule ◽  
Scanning Electron

Although there have been a number of recent scanning electron microscopic reports on the renal glomerulus, the advantages of scanning electron microscopy have not yet been applied to a systematic study of the uriniferous tubules. In the present investigation, scanning electron microscopy was used to study the ultrastructural morphology of the proximal, distal, thin loop, and collecting tubules. Material for observation was taken from rat kidneys which were fixed by vascular perfusion, sectioned by either cutting or fracturing technigues, and critically point dried.The brush border characterising proximal tubules is first detected on the luminal surface of Bowman's capsule adjacent to the urinary pole orifice. In this region one frequently finds irregular microvilli characterized by broad and flattened bases with occasional bulbous structures protruding from their surfaces.

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