Influence of deep‐freezing and MGG staining on DNA and RNA quality in different types of lung adenocarcinoma cytological smears

2021 ◽  
Author(s):  
Suzana Harabajsa ◽  
Valerija Begić ◽  
Paula Gršković ◽  
Vesna Šimić ◽  
Božica Vrabec Branica ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
Deep Freezing ◽  
Rna Quality ◽  
Dna And Rna ◽  
Different Types

Abstract A43: Identification of key tumorigenic pathways in never-smoker lung adenocarcinoma patients using massively parallel DNA and RNA sequencing

2012 ◽  
Vol 18 (3 Supplement) ◽  
pp. A43-A43
Author(s):  
Timothy G. Whitsett ◽  
Shripad Sinari ◽  
Kelly W. Sheff ◽  
Phillip Y. Cheung ◽  
Julianna Ross ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
Rna Sequencing ◽  
Massively Parallel ◽  
Dna And Rna ◽  
Never Smoker

Effects of the antioestrogen tamoxifen on steroid induced morphological and biochemical changes in the castrated dog prostate

1982 ◽  
Vol 100 (3) ◽  
pp. 462-472 ◽  
Author(s):  
P.-J. Funke ◽  
U. W. Tunn ◽  
Th. Senge ◽  
F. Neumann
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Biochemical Analysis ◽  
Squamous Metaplasia ◽  
Combined Treatment ◽  
Prostatic Hyperplasia ◽  
Biochemical Changes ◽  
Weight Increase ◽  
Histochemical Reaction ◽  
Dna And Rna ◽  
Different Types

Abstract. The effect of the antioestrogen tamoxifen (TA) was investigated in different types of steroid-induced benign prostatic hyperplasia (BPH) in the castrated dog by histological, histochemical and biochemical analysis. A 6 months treatment with oestradiol-17β (E2) alone resulted in cystic and stromal hyperplasia and squamous epithelial metaplasia with a striking prostatic weight increase. DNA and RNA content of the total glands increased significantly. The histochemical results and zinc values indicated the loss of normal epithelial function due to metaplatic transformation. The E2 induced cystic and metaplastic hyperplasia was prevented by TA while the stromal proliferation was significantly decreased but not abolished. Biochemical determinations revealed an effect similar to castration. After combined treatment with E2 and 3α-androstanediol (3α-diol) TA completely suppressed squamous metaplasia. A 3α-diol induced glandular proliferation, monitored by a positive histochemical reaction, and significantly elevated zinc, DNA and RNA contents prevailed. A partial stromal stimulation indicates stimulating effects of 3α-diol too on the stroma. The antioestrogenic effects of tamoxifen on experimentally induced BPH mainly manifest at the E2 induced epithelial alterations. The abolishing effects at the stromal level are distinct but not so impressive.

scholarly journals Correlation between expression of Ki-67 and MSCT signs in different types of lung adenocarcinoma

Medicine ◽  
2020 ◽  
Vol 99 (2) ◽  
pp. e18678
Author(s):  
Jingang Yan ◽  
Heping Wang ◽  
Haiwei Zhou ◽  
Hui He ◽  
Lin Qiu ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
Ki 67 ◽  
Different Types

scholarly journals Maternal microbiome during pregnancy and their impact on the canine microbiome in neonates

2020 ◽  
Vol 51 (6) ◽  
pp. 593-604
Author(s):  
Maja Zakošek Pipan ◽  
Janko Mrkun
Keyword(s):  
Oral Cavity ◽  
Gut Microbiota ◽  
Amniotic Fluid ◽  
Current Knowledge ◽  
Bacterial Dna ◽  
Dna And Rna ◽  
Different Types ◽  
Human Neonates

The long-standing hypothesis that the neonatal microbiome is acquired during and after birth has been challenged by recent studies in humans using advanced molecular technologies, which identified bacteria and bacterial DNA and RNA in amniotic fluid, placenta, and meconium. Similar studies have recently been conducted on canine neonates, giving a description of the gut microbiota of newborn puppies and a comparison of different types of parturition on theirdevelopment. In this review, we summarize the current knowledge of microbiome and microbial alterations in the canine gut, vagina and oral cavity throughout pregnancy, and their effects on the microbiome of the placenta and the meconium in neonatal puppies. We further compare the microbiome knowledge gained in the last decade on human neonates and draw parallels with canine pregnancy and the neonatal microbiome.

scholarly journals An Overview of the Oncogenic Signaling Pathways in Different Types of Cancers

2020 ◽  
Author(s):  
Afshin Derakhshani ◽  
Zeinab Rostami ◽  
Sina Taefehshokr ◽  
Hossein Safarpour ◽  
Reza Vaezi Astamal ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Regulatory Mechanisms ◽  
Epigenetic Changes ◽  
Oncogenic Signaling ◽  
Genetic Changes ◽  
Rna Analysis ◽  
Dna And Rna ◽  
Different Types ◽  
Recent Developments ◽  
Oncogenic Signaling Pathways

Cancer is a leading cause of death worldwide. It is theorized that underlying genetic and epigenetic changes enable cells to proliferate out of control by escaping regulatory mechanisms. The progression of cancer is associated with increased cell proliferation, metabolic modifications, resistance to apoptosis, genetic instability, induction of angiogenesis and augmented migratory capability. Recent developments in DNA and RNA analysis have made it possible to study these genetic changes systematically. These advances have enabled us to possess a deeper knowledge of the signaling pathways and involved processes. In-depth studies of the pathways involved in carcinogenesis have led to the identification of pathways that may be targeted for therapeutic purposes. In this review, we provide an overview of the relevant mechanisms and pathways involved in the development and progression of cancer.

scholarly journals Compared DNA and RNA quality of breast cancer biobanking samples after long-term storage protocols in − 80 °C and liquid nitrogen

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Maximilian Babel ◽  
Andreas Mamilos ◽  
Stephan Seitz ◽  
Tanja Niedermair ◽  
Florian Weber ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Liquid Nitrogen ◽  
Rna Quality ◽  
Dna And Rna ◽  
Long Term Storage ◽  
Term Storage

scholarly journals The Biospecimen Preanalytical Variables Program: A Multiassay Comparison of Effects of Delay to Fixation and Fixation Duration on Nucleic Acid Quality

2019 ◽  
Vol 143 (9) ◽  
pp. 1106-1118 ◽  
Author(s):  
Latarsha J. Carithers ◽  
Rachana Agarwal ◽  
Ping Guan ◽  
Hana Odeh ◽  
Michael C. Sachs ◽  
...  
Keyword(s):  
Rna Integrity ◽  
Time Points ◽  
Rna Quality ◽  
Rna Integrity Number ◽  
Dna And Rna ◽  
Specific Effects ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Preanalytical Variability ◽  
Tumor Types

Context.—Despite widespread use of formalin-fixed, paraffin-embedded (FFPE) tissue in clinical and research settings, potential effects of variable tissue processing remain largely unknown.Objective.—To elucidate molecular effects associated with clinically relevant preanalytical variability, the National Cancer Institute initiated the Biospecimen Preanalytical Variables (BPV) program.Design.—The BPV program, a well-controlled series of systematic, blind and randomized studies, investigated whether a delay to fixation (DTF) or time in fixative (TIF) affects the quantity and quality of DNA and RNA isolated from FFPE colon, kidney, and ovarian tumors in comparison to case-matched snap-frozen controls.Results.—DNA and RNA yields were comparable among FFPE biospecimens subjected to different DTF and TIF time points. DNA and RNA quality metrics revealed assay- and time point–specific effects of DTF and TIF. A quantitative reverse transcription–polymerase chain reaction (qRT-PCR) assay was superior when assessing RNA quality, consistently detecting differences between FFPE and snap-frozen biospecimens and among DTF and TIF time points. RNA Integrity Number and DV200 (representing the percentage of RNA fragments longer than 200 nucleotides) displayed more limited sensitivity. Differences in DNA quality (Q-ratio) between FFPE and snap-frozen biospecimens and among DTF and TIF time points were detected with a qPCR-based assay.Conclusions.—DNA and RNA quality may be adversely affected in some tumor types by a 12-hour DTF or a TIF of 72 hours. Results presented here as well as those of additional BPV molecular analyses underway will aid in the identification of acceptable delays and optimal fixation times, and quality assays that are suitable predictors of an FFPE biospecimen's fit-for-purpose.

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