Prevalence and typing of HPV DNA by hybrid capture II in women with ASCUS, ASC-H, LSIL, and AGC on ThinPrep® Pap tests

2004 ◽  
Vol 30 (6) ◽  
pp. 426-432 ◽  
Author(s):  
Leslie R. Rowe ◽  
William Aldeen ◽  
Joel S. Bentz
Keyword(s):  
Pap Tests ◽  
Hybrid Capture ◽  
Hpv Dna

scholarly journals Comparison of the Hybrid Capture II Method with a PCR-Based Screening Method Using a Carboxyfluorescein-Labeled Primer for Detecting Human Papillomavirus in Cervicovaginal Liquid-Based Cytology

2020 ◽  
Vol 1 (1) ◽  
pp. 9-18
Author(s):  
Yusuke Saiki ◽  
Yuka Gion ◽  
Asami Nishikori ◽  
Yoshiaki Norimatsu ◽  
Yasuharu Sato
Keyword(s):  
Screening Method ◽  
Human Papillomaviruses ◽  
Screening Methods ◽  
Consensus Primer ◽  
Perfect Agreement ◽  
Diagnostic Reliability ◽  
Pap Tests ◽  
Hybrid Capture ◽  
Hpv Dna ◽  
Liquid Based Cytology

Objective: Human papillomaviruses (HPVs) are DNA viruses, of which over 120 types have been identified. The main screening methods for HPV-DNA include the hybrid capture II (HC-II) and polymerase chain reaction (PCR) assays. Liquid-based cytology (LBC) is a high-quality technique developed to improve the diagnostic reliability of traditional Papanicolaou tests (Pap tests). However, relatively few studies have compared the efficacy of PCR and HC-II assays using cervicovaginal LBC specimens. In this study, we conducted a comparative analysis with results derived from the HC-II assay to assess whether a PCR-based assay using a novel carboxyfluorescein (FAM)-labeled primer could be applied to cervicovaginal LBC specimens. Methods and Results: We analyzed 59 specimens diagnosed as atypical squamous cells of undetermined significance (ASCUS) by Pap tests. After extracting DNA from cervicovaginal LBC specimens, we performed PCR using a FAM-labeled consensus primer, and then conducted fragment analysis to confirm the results. The value of the kappa statistic measuring the agreement between the PCR and HC-II results was 0.8557, or “almost perfect agreement.” Conclusion: Our novel HPV-PCR assay can be successfully applied to cervicovaginal LBC specimens for the detection of HPV subtypes.

Comparison of Abbott RealTime High Risk HPV and Hybrid Capture 2 for the detection of high-risk HPV DNA in a referral population setting

2012 ◽  
Vol 53 (2) ◽  
pp. 121-124 ◽  
Author(s):  
Simona Venturoli ◽  
Elisa Leo ◽  
Martina Nocera ◽  
Daniela Barbieri ◽  
Monica Cricca ◽  
...  
Keyword(s):  
High Risk ◽  
Hybrid Capture ◽  
Hpv Dna ◽  
Hybrid Capture 2 ◽  
High Risk Hpv

scholarly journals Usefulness of Hybrid Capture HPV DNA Assay as a Diagnostic Tool for Human Papillomavirus Infection

1998 ◽  
Vol 72 (11) ◽  
pp. 1219-1224 ◽  
Author(s):  
Takeyoshi KUBOTA ◽  
Hisashi ISHIKAZU ◽  
Masaaki SUZUKI ◽  
Sakae UTSUNO ◽  
Jun IGARI
Keyword(s):  
Human Papillomavirus ◽  
Diagnostic Tool ◽  
Hybrid Capture ◽  
Hpv Dna ◽  
Dna Assay ◽  
Human Papillomavirus Infection ◽  
Papillomavirus Infection

Human Papillomavirus Detection by Hybrid Capture II and Residual or Recurrent High-Grade Squamous Cervical Intraepithelial Neoplasia after Large Loop Excision of the Transformation Zone (LLETZ)

2005 ◽  
Vol 91 (2) ◽  
pp. 188-192 ◽  
Author(s):  
Luís Otávio Sarian ◽  
Sophie Françoise Mauricette Derchain ◽  
Denise da Rocha Pitta ◽  
Liliana Aparecida Angelo Andrade ◽  
Sirlei Siani Moráis ◽  
...  
Keyword(s):  
Cervical Intraepithelial Neoplasia ◽  
Dna Detection ◽  
Intraepithelial Neoplasia ◽  
High Grade ◽  
Transformation Zone ◽  
Hybrid Capture ◽  
Hpv Dna ◽  
Large Loop ◽  
Gynecological Examination

Aims and background The purpose of this study was to assess the association between highly-oncogenic types HPV DNA detection by Hybrid Capture II (HCII) and residual or recurrent high-grade cervical intraepithelial neoplasia (CIN 2 or 3) during the follow-up of women submitted to large loop excision of the transformation zone (LLETZ). Study design In this cohort study, 94 women submitted to LLETZ because of CIN 2 or 3 between March 2001 and September 2002 were followed up twice yearly until September 2003. Follow-up visits consisted of an interview regarding clinical, social and demographic characteristics complemented with gynecological examination with specimen collection for Pap test and HCII and colposcopy. Eighty-one patients attended the first visit (mean 4.8 months, range 3-6) and 75 the second visit (mean 10.9 months, range 7-17 months). McNemar's test to assess the variation of HPV DNA detection following LLETZ, odds ratios (OR) to evaluate the correlation between HPV DNA positivity and residual/recurrent CIN during follow-up, and logistic regression to assess the risk of residual/recurrent CIN were used. Results There was a strong and significant reduction in HPV detection after LLETZ ( P <0.001). HPV DNA detection was correlated with residual/recurrent CIN at the first (OR = 103.4; 95% CI 5.5 to 1961.2) and second (OR = 12.7; 95% CI 1.1 to 345.5) follow-up visits. Multivariate analysis showed HPV persistence as a stand-alone risk factor for residual/recurrent CIN (OR = 50.3; 95% CI 3.8 to 663.1). Conclusions High risk HPV DNA detection decreased substantially after CIN treatment with LLETZ, but HPV persistence was strongly correlated with residual/recurrent CIN.

scholarly journals Detection of human papillomavirus in cervical cell specimens by hybrid capture and PCR with different primers.

2006 ◽  
Vol 53 (3) ◽  
pp. 603-607 ◽  
Author(s):  
Slawa Szostek ◽  
Malgorzata Klimek ◽  
Barbara Zawilinska ◽  
Janusz Rys ◽  
Jolanta Kope ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Consensus Primer ◽  
Hybrid Capture ◽  
Hpv Dna ◽  
Cervical Smears ◽  
Capture Assay ◽  
Cervical Cell ◽  
Target Dna ◽  
Primer Sets

The purpose of this study was to compare hybrid capture assay with PCRs using different primers for the L1, E6-E7 regions for the detection of human papillomavirus (HPV) genome. One hundred twenty-five cervical smears with normal (n=42) and abnormal (n=83) cytology were investigated. Those at high-risk for HPV were studied by hybridization antibody capture assay and PCR with the pU-1M/pU-2R primers. Target DNA from the HPV L1 region was amplified by SPF10 primer set and home-PCR with MY09/MY11 primers. The presence of HPV DNA in cervical smears was detected by SPF10 (in 72% of cases), MY09/MY11 (58%), hybrid capture (55%) and pU-1M/pU-2R (39%). Results obtained with the SPF10 and MY09/MY11 consensus primer sets as well as hybrid capture and pU-1M/pU-2R specific for high-risk types differed significantly (chi2, P

scholarly journals Human Papilloma Virus: Biomolecular Aspect

2021 ◽  
Vol 5 (3) ◽  
pp. 634-641
Author(s):  
Patiyus Agustiansyah ◽  
Rizal Sanif ◽  
Siti Nurmaini ◽  
Irfannuddin ◽  
Legiran
Keyword(s):  
High Risk ◽  
Visual Inspection ◽  
Pap Smear ◽  
Precancerous Lesions ◽  
Papilloma Virus ◽  
Hybrid Capture ◽  
Hpv Dna ◽  
Sexually Transmitted ◽  
Risk Of Progression ◽  
High Risk Hpv

HPV is a sexually transmitted virus, and high-risk HPV DNA was found in 99.7%of cervical cancer specimens. Within 12 to 24 months of exposure to the virus,90% of HPV infections disappear or become inactive. However, infection with high-risk strains of HPV persist which then increases the risk of progression to cervicalcancer. The detection of precancerous lesions consists of various methods,including pap smear (conventional or liquid-base cytology / LBC), visualinspection of acetic acid (IVA), visual inspection of lugoliodine (VILI), and HPV DNAtest (genotyping / hybrid capture).

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