Primary diagnosis and REAL/WHO classification of non-Hodgkin's lymphoma by fine-needle aspiration: Cytomorphologic and immunophenotypic approach

2003 ◽  
Vol 28 (4) ◽  
pp. 191-195 ◽  
Author(s):  
W.A. Mourad ◽  
A. Tulbah ◽  
M. Shoukri ◽  
F. Al Dayel ◽  
M. Akhtar ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Hodgkin’S Lymphoma ◽  
Who Classification ◽  
Needle Aspiration ◽  
Primary Diagnosis ◽  
Hodgkin's Lymphoma ◽  
Fine Needle ◽  
Non Hodgkin’S Lymphoma ◽  
Non Hodgkin's Lymphoma

scholarly journals Immunophenotyping of non-Hodgkin’s lymphoma by flowcytometry on fine needle aspiration

2021 ◽  
Vol 9 (10) ◽  
pp. 2976
Author(s):  
Sanjay Kumar ◽  
Meena Verma ◽  
Pinki Devi ◽  
Sant P. Kataria ◽  
Rajeev Sen
Keyword(s):  
Fine Needle Aspiration ◽  
Hodgkin’S Lymphoma ◽  
Needle Aspiration ◽  
Hodgkin's Lymphoma ◽  
Fine Needle ◽  
Non Hodgkin’S Lymphoma ◽  
Non Hodgkin's Lymphoma ◽  
Mantle Cell ◽  
Needle Aspiration Cytology ◽  
Reactive Hyperplasia

Background: Lymphoma represents one of the major health problems all over the world. Flow cytometry (FCM) can be used on fine-needle aspiration cytology (FNAC) from lymph node as an ancillary technique. Aim of the study was to assess the utility of flowcytometry (FCM) in diagnosis and differentiation of reactive hyperplasia and non-Hodgkin’s lymphoma (NHL) on FNAC.Methods: The study was carried out on 50 cases, 25 each of reactive hyperplasia and suspicious or confirmed NHL on FNAC. FCI was performed with a complete panel of antibodies on FACS Canto II FCM.Results: All 25 cases of reactive hyperplasia on FNAC were polyclonal on FCM. FCM could be performed in 22 cases (88%) out of 25 suspicious NHL and in three cases the material was inadequate on aspirate. Out of 22 cases of NHL on FNAC 17 cases (77.30%) were diagnosed as B-NHL on FCM. Light chain restriction was demonstrated in 15/17 cases. With the help of FCI, 6 cases were diagnosed as small cell lymphocytic lymphoma, one case as mantle cell lymphoma, one case as follicular lymphoma, and 9 cases as B-NHL-NOS. Histopathology diagnosis was available in nine cases and were in concordance to FCM. Sensitivity of combined FNAC and FCM in sub-classification was 77.30% (17/22). Four cases showed discordance between FNAC and FCM.Conclusion: We concluded that FCM enhances the diagnostic ability of FNAC, playing a crucial role in a rapid and accurate differential diagnosis between reactive hyperplasia, B-NHL and T-NHL.

Distribution and Pathology Characteristics of Non Hodgkin Lymphoma in Peru: A Study of 1014 Cases Using WHO Classification of Lymphoid Neoplasm.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 4419-4419 ◽  
Author(s):  
Brady Beltran ◽  
Domingo Morales ◽  
Pilar Quiñones ◽  
R. Salas ◽  
Antonio A. Carrasco-Yalan
Keyword(s):  
T Cell ◽  
Hodgkin’S Lymphoma ◽  
Who Classification ◽  
Hodgkin's Lymphoma ◽  
Asian Countries ◽  
Non Hodgkin’S Lymphoma ◽  
T Cell Lymphomas ◽  
Non Hodgkin's Lymphoma ◽  
Indolent Lymphomas

Abstract BACKGROUND: The frequency of various subtypes of non-Hodgkin’s lymphoma (NHL) differs in various regions worldwide. OBJECTIVE: To investigate the clinical and pathological features of non-Hodgkin’s lymphoma (NHL) and to evaluate the applicability of the new WHO classification of lymphoid neoplasms. METHODS: According to the new WHO classification, a total of 1014 cases of non-Hodgkin’s lymphoma diagnosed during the period 2002–2006 were reviewed and reappraised with their morphological, immunological and clinical characteristics in one general hospital from Lima, Peru. All cases corresponded >18 years old. RESULTS: There were 535 males and 479 females, mean age was 62.1 years (range 18 – 97 years) and the median was 64 years. B-cell neoplasms accounted for 763 cases (75.2%) and T/NK-cell neoplasms for 189 (18.6%). Sixty two cases (6.1%) were not classified. It was seen compared to that in the other Asian countries. Indolent lymphomas accounted for 17%, and aggressive ones for 83%. Among indolent lymphomas follicular grade I and II were the most common subset while MALT was second with low frequency. Among the aggressive lymphomas diffuse large cell lymphoma (DLCL) was the most common subtype, and accounted for 58.8% of all B cell lymphomas. Mantle and Burkitt lymphoma were very low incidence. Among the T cell lymphomas, peripheral T cell lymphomas, mycosis fungoides, Adult T Lymphoma/Leukemia (ATLL), T/NK nasal type Lymphoma were the most common subtypes. Nodal NHL occurred in 52% and extranodal in 48% of the cases. The more common extranodal presentation was stomach (14.1%), skin (8.1%), small intestine (2.9%) and nose (2.3%) CONCLUSIONS: The high incidence of T cell lymphomas, extranodal presentation and reduced frequency of indolent lymphoma in the current study is comparable to that reported from Asian countries.

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