scholarly journals Thirty-five years of CD4 T-cell counting in HIV infection: From flow cytometry in the lab to point-of-care testing in the field

2016 ◽  
Vol 92 (6) ◽  
pp. 437-444 ◽  
Author(s):  
L. Kestens ◽  
F. Mandy
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
Hiv Infection ◽  
Point Of Care ◽  
Cd4 T Cell ◽  
Cell Counting ◽  
Point Of Care Testing

scholarly journals Preclinical Assessment of a Cartridge-Based Flow-Through Assay for Determination of Adult CD4 T-Cell Count

2020 ◽  
Vol 14 (1) ◽  
pp. 50-60
Author(s):  
Simon Bystryak ◽  
Chitrangada Acharya ◽  
Kyle Dobiszewski ◽  
Hongying Zhu ◽  
Rajiv P. Bandwar
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
Cell Count ◽  
Point Of Care ◽  
Cd4 Count ◽  
Cd4 T Cell ◽  
Cell Counting ◽  
Wide Range ◽  
Flow Through ◽  
Cd4 T Cell Count

Background: Despite the emphasis on viral load testing, current HIV testing guidelines consider CD4 T-cell count measurement as an important criterion for assessing disease progression, making decisions about anti-retroviral therapy regime changes, and treating HIV infected individuals with opportunistic infections. The CD4 counting by established methods (e.g., flow cytometry) presents challenges not only in resource-scarce settings due to cost and lack of skilled technicians but also in resource-rich areas where it is limited to centralized facilities. Objective: Current options for Point-Of-Care (POC) CD4 enumeration are few and labor-intensive, prompting the need for newer technological methods that can overcome the aforementioned challenges. Methods: The novel and patented flow-through cell counting assay (FTCA) described previously (Bystryak et al., 2019) was developed further into a point-of-care CD4 testing system using a disposable cartridge device and a portable imaging instrument. A pilot study with ~100 samples using this device was conducted to assess the validity of FTCA as a POC test for the measurement of CD4 count. Results: The FTCA signal was found to be linear over a wide range (17 - 1540 cells/μL) of CD4 T-cell concentration. The FTCA method also exhibits a strong agreement with flow cytometry, with very low bias (− 7 cells/μL) towards CD4 count measurement. Conclusion: The cartridge-based FTCA method has great potential to be a fully quantitative method with low complexity, portability, low-cost, and wide applicability in clinical practice.

scholarly journals Enhancing the performance of a point-of-care CD4+ T-cell counting microchip through monocyte depletion for HIV/AIDS diagnostics

Lab on a Chip ◽  
2009 ◽  
Vol 9 (10) ◽  
pp. 1357 ◽  
Author(s):  
Xuanhong Cheng ◽  
Amit Gupta ◽  
Chihchen Chen ◽  
Ronald G. Tompkins ◽  
William Rodriguez ◽  
...  
Keyword(s):  
T Cell ◽  
Point Of Care ◽  
Cd4 T Cell ◽  
Cell Counting ◽  
Hiv Aids

Accurate CD4 T-cell enumeration and antiretroviral drug toxicity monitoring in primary healthcare clinics using point-of-care testing

AIDS ◽  
2011 ◽  
Vol 25 (6) ◽  
pp. 807-812 ◽  
Author(s):  
Ilesh V Jani ◽  
Nádia E Sitoe ◽  
Patrina L Chongo ◽  
Eunice R Alfai ◽  
Jorge I Quevedo ◽  
...  
Keyword(s):  
T Cell ◽  
Primary Healthcare ◽  
Drug Toxicity ◽  
Point Of Care ◽  
Cd4 T Cell ◽  
Point Of Care Testing ◽  
Toxicity Monitoring ◽  
Antiretroviral Drug ◽  
Cell Enumeration ◽  
Primary Healthcare Clinics

scholarly journals Evaluation of the FACSPresto, a New Point of Care Device for the Enumeration of CD4% and Absolute CD4+ T Cell Counts in HIV Infection

PLoS ONE ◽  
2016 ◽  
Vol 11 (7) ◽  
pp. e0157546 ◽  
Author(s):  
Azure Tariro Makadzange ◽  
Carola Bogezi ◽  
Kathryn Boyd ◽  
Anesu Gumbo ◽  
Dorinda Mukura ◽  
...  
Keyword(s):  
T Cell ◽  
Hiv Infection ◽  
Point Of Care ◽  
Cd4 T Cell ◽  
Cell Counts

The Effect of Antiretroviral Therapy on IL-6, IL-1β, TNF-α, IFN-γ Levels and their Relationship with HIV-RNA and CD4+ T Cells in HIV Patients

2020 ◽  
Vol 18 (5) ◽  
pp. 354-361
Author(s):  
Gülay Okay ◽  
Meliha Meric Koc ◽  
Eray Metin Guler ◽  
Ayşegül Yabaci ◽  
Abdürrahim Kocyigit ◽  
...  
Keyword(s):  
T Cell ◽  
Hiv Infection ◽  
Cell Count ◽  
Cd4 T Cell ◽  
Positive Correlation ◽  
Hiv Rna ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Ifn Γ ◽  
Cd4 T Cell Count

Background: Serum cytokine levels over the course of HIV infection usually increase with immunosuppression and decrease after antiretroviral treatment (ART). Objectives: The aim of the study is to compare cytokine levels between HIV-infected patients (HIP) and controls and investigate the relationship between CD4+T cell count, HIV-RNA levels, and cytokine levels. Methods: The study subjects comprised ART-naive HIP (n=30) with no comorbidities and age-and sex-matched healthy controls. We measured levels of IL-6, IL-1β, TNF-α, and IFN-γ in serum samples of HIP at the beginning and at month 6 of ART and in controls. Results: The mean age of the study subjects was 38.7 ±10.3 years, with men making up 86.7% of the study subjects (n=26). IL-6, IL-1β, and TNF-α levels were significantly higher in both ART-naive (p<0.001, p=0.002, p=0.001) and ART-experienced HIP (p<0.001) than controls. The IFN-γ level was lower in both ART-naive and ART-experienced HIP compared to controls (p=0.082 and p=0.002). There was a positive correlation between the CD4+T cell count and serum concentration of IFN- γ(r=0.320, p<0.05). While the serum IFN-γ concentration showed a negative correlation with the HIVRNA level(r=-0.412, p<0.001), the serum IL-1β, IL-6, and TNF-α concentrations showed a positive correlation with the HIV-RNA level (r=0.349, p<0.001; r:0.54, p<0.001; r:0.438, p<0.00). Conclusions: Although serum concentrations of IL-6, IL-1β and TNF-α showed a significant decrease after ART, they were still significantly higher than the controls. IFN-γ responded differently to ART compared to the other cytokines, indicating that it may play a distinct and important role in the pathogenesis of HIV infection.

scholarly journals Comparison of Lethal and Nonlethal Mouse Models of Orientia tsutsugamushi Infection Reveals T-Cell Population-Associated Cytokine Signatures Correlated with Lethality and Protection

2021 ◽  
Vol 6 (3) ◽  
pp. 121
Author(s):  
Alison Luce-Fedrow ◽  
Suchismita Chattopadhyay ◽  
Teik-Chye Chan ◽  
Gregory Pearson ◽  
John B. Patton ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
Host Response ◽  
Cd8 T Cell ◽  
Cd4 T Cell ◽  
Cell Populations ◽  
Murine Models ◽  
Orientia Tsutsugamushi ◽  
Multicolor Flow Cytometry ◽  
Interstrain Difference

The antigenic diversity of Orientia tsutsugamushi as well as the interstrain difference(s) associated with virulence in mice impose the necessity to dissect the host immune response. In this study we compared the host response in lethal and non-lethal murine models of O. tsutsugamushi infection using the two strains, Karp (New Guinea) and Woods (Australia). The models included the lethal model: Karp intraperitoneal (IP) challenge; and the nonlethal models: Karp intradermal (ID), Woods IP, and Woods ID challenges. We monitored bacterial trafficking to the liver, lung, spleen, kidney, heart, and blood, and seroconversion during the 21-day challenge. Bacterial trafficking to all organs was observed in both the lethal and nonlethal models of infection, with significant increases in average bacterial loads observed in the livers and hearts of the lethal model. Multicolor flow cytometry was utilized to analyze the CD4+ and CD8+ T cell populations and their intracellular production of the cytokines IFNγ, TNF, and IL2 (single, double, and triple combinations) associated with both the lethal and nonlethal murine models of infection. The lethal model was defined by a cytokine signature of double- (IFNγ-IL2) and triple-producing (IL2-TNF-IFNγ) CD4+ T-cell populations; no multifunctional signature was identified in the CD8+ T-cell populations associated with the lethal model. In the nonlethal model, the cytokine signature was predominated by CD4+ and CD8+ T-cell populations associated with single (IL2) and/or double (IL2-TNF) populations of producers. The cytokine signatures associated with our lethal model will become depletion targets in future experiments; those signatures associated with our nonlethal model are hypothesized to be related to the protective nature of the nonlethal challenges.

CD4+ T cell count, HIV-1 viral loads and demographic variables of newly identified patients with HIV infection in Wuhan, China

2013 ◽  
Vol 85 (10) ◽  
pp. 1687-1691 ◽  
Author(s):  
Man-Qing Liu ◽  
Li Tang ◽  
Wen-Hua Kong ◽  
Ze-Rong Zhu ◽  
Jin-Song Peng ◽  
...  
Keyword(s):  
T Cell ◽  
Hiv Infection ◽  
Cell Count ◽  
Demographic Variables ◽  
Cd4 T Cell ◽  
Viral Loads ◽  
Hiv 1 ◽  
Cd4 T Cell Count
Sign in / Sign up

Export Citation Format

Share Document