scholarly journals Recommendations for the development and validation of flow cytometry-based receptor occupancy assays

2016 ◽  
Vol 90 (2) ◽  
pp. 141-149 ◽  
Author(s):  
Cherie L. Green ◽  
Jennifer J. Stewart ◽  
Carl-Magnus Högerkorp ◽  
Alan Lackey ◽  
Nicholas Jones ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Receptor Occupancy ◽  
Development And Validation

A Simple and Rapid Method to Determine GPIIb/IIIa-Receptor Inhibitor Activity in Whole Blood

1999 ◽  
Vol 19 (03) ◽  
pp. 134-138
Author(s):  
Gitta Kühnel ◽  
A. C. Matzdorff
Keyword(s):  
Flow Cytometry ◽  
Platelet Count ◽  
Blood Sample ◽  
Platelet Activation ◽  
Whole Blood ◽  
Receptor Occupancy ◽  
Aggregate Formation ◽  
Inhibitor Activity ◽  
Receptor Inhibitor

SummaryWe studied the effect of GPIIb/IIIa-inhibitors on platelet activation with flow cytometry in vitro. Citrated whole blood was incubated with increasing concentrations of three different GPIIb/IIIa-inhibitors (c7E3, DMP728, XJ757), then thrombin or ADP were added and after 1 min the sample was fixed. Samples without c7E3 but with 0.1 U/ml thrombin had a decrease in platelet count. Samples with increasing concentrations of c7E3 had a lesser or no decrease in platelet count. The two other inhibitors (DMP 725, XJ757) gave similar results. GPIIb/IIIa-inhibitors prevent aggregate formation and more single platelets remain in the blood sample. The agonist-induced decrease in platelet count correlates closely with the concentration of the GPIIb/IIIa inhibitor and receptor occupancy. This correlation may be used as a simple measure for inhibitor activity in whole blood.

scholarly journals Flow cytometry and receptor occupancy in immune-oncology therapy

2021 ◽  
Author(s):  
Alessandra Audia ◽  
Gregory Bannish ◽  
Rachel Bunting ◽  
Chelsea Riveley
Keyword(s):  
Flow Cytometry ◽  
Receptor Occupancy ◽  
Immune Oncology

scholarly journals Receptor occupancy assessment by flow cytometry as a pharmacodynamic biomarker in biopharmaceutical development

2015 ◽  
Vol 90 (2) ◽  
pp. 117-127 ◽  
Author(s):  
Meina Liang ◽  
Martin Schwickart ◽  
Amy K. Schneider ◽  
Inna Vainshtein ◽  
Christopher Del Nagro ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Receptor Occupancy

P143 Development and validation of LSAB MFI cutpoints for use in the virtual crossmatch test to predict flow cytometry crossmatch (FCXM) and cdc crossmatch (CDC XM) results

2018 ◽  
Vol 79 ◽  
pp. 167
Author(s):  
Vijay K. Sharma ◽  
Rex Friedlander ◽  
Sue Ayelet Tiongko ◽  
Joshua Kahane ◽  
Nora Alzahrani ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Development And Validation

scholarly journals Development and validation of a bioassay to evaluate binding of adalimumab to cell membrane-anchored TNFα using flow cytometry detection

2018 ◽  
Vol 155 ◽  
pp. 235-240
Author(s):  
Rosa Camacho-Sandoval ◽  
Eréndira N. Sosa-Grande ◽  
Edith González-González ◽  
Alejandra Tenorio-Calvo ◽  
Carlos A. López-Morales ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Membrane ◽  
Flow Cytometry Detection ◽  
Development And Validation

scholarly journals The development and validation of different decision-making tools to predict urine culture growth out of urine flow cytometry parameter

PLoS ONE ◽  
2018 ◽  
Vol 13 (2) ◽  
pp. e0193255 ◽  
Author(s):  
Martin Müller ◽  
Ruth Seidenberg ◽  
Sabine K. Schuh ◽  
Aristomenis K. Exadaktylos ◽  
Clyde B. Schechter ◽  
...  
Keyword(s):  
Decision Making ◽  
Flow Cytometry ◽  
Urine Culture ◽  
Urine Flow ◽  
Culture Growth ◽  
Development And Validation ◽  
Urine Flow Cytometry
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