scholarly journals Current laboratory practices in flow cytometry for the enumeration of CD 4+T-lymphocyte subsets

2015 ◽  
Vol 88 (5) ◽  
pp. 305-311 ◽  
Author(s):  
L. Whitby ◽  
A. Whitby ◽  
M. Fletcher ◽  
D. Barnett
Keyword(s):  
Flow Cytometry ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
T Lymphocyte Subsets

scholarly journals Flow Cytometry Study of Lymphocyte Subsets in Malnourished and Well-Nourished Children with Bacterial Infections

2004 ◽  
Vol 11 (3) ◽  
pp. 577-580 ◽  
Author(s):  
Oralia Nájera ◽  
Cristina González ◽  
Guadalupe Toledo ◽  
Laura López ◽  
Rocío Ortiz
Keyword(s):  
Flow Cytometry ◽  
B Lymphocytes ◽  
T Lymphocyte ◽  
Bacterial Infections ◽  
B Lymphocyte ◽  
Lymphocyte Subsets ◽  
Protein Energy Malnutrition ◽  
T Lymphocyte Subsets ◽  
Malnourished Children ◽  
Protein Energy

ABSTRACT Protein-energy malnutrition is the primary cause of immune deficiency in children across the world. It has been related to changes in peripheral T-lymphocyte subsets. The aim of the present study was to evaluate the effects of infection and malnutrition on the proportion of peripheral-lymphocyte subsets in well-nourished non-bacterium-infected (WN), well-nourished bacterium-infected (WNI), and malnourished bacterium-infected (MNI) children by flow cytometry. A prospectively monitored cohort of 15 MNI, 12 WNI, and 17 WN children was studied. All the children were 3 years old or younger and had only bacterial infections. Results showed a significant decrease in the proportion of T CD3+ (P < 0.05 for relative and P < 0.03 for absolute values), CD4+ (P < 0.01 for relative and absolute values), and CD8+ (P < 0.05 for relative values) lymphocyte subsets in WNI children compared to the results seen with WN children. Additionally, B lymphocytes in MNI children showed significant lower values (CD20+ P < 0.02 for relative and P < 0.05 for absolute values) in relation to the results seen with WNI children. These results suggest that the decreased proportions of T-lymphocyte subsets observed in WNI children were associated with infection diseases and that the incapacity to increase the proportion of B lymphocyte was associated with malnutrition. This low proportion of B lymphocytes may be associated with the mechanisms involved in the immunodeficiency of malnourished children.

scholarly journals Effects of moderate and severe malnutrition in rats on splenic T lymphocyte subsets and activation assessed by flow cytometry

2008 ◽  
Vol 152 (3) ◽  
pp. 585-592 ◽  
Author(s):  
E. Cortés-Barberena ◽  
H. González-Márquez ◽  
J. L. Gómez-Olivares ◽  
R. Ortiz-Muñiz
Keyword(s):  
Flow Cytometry ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Severe Malnutrition ◽  
T Lymphocyte Subsets

Two-Color Flow Cytometry Analysis of Activated T-Lymphocyte Subsets in Type I Diabetes Mellitus

Diabetes ◽  
1988 ◽  
Vol 37 (6) ◽  
pp. 792-795 ◽  
Author(s):  
C. M. Legendre ◽  
A. Schiffrin ◽  
G. Weitzner ◽  
E. Colle ◽  
R. D. Guttmann
Keyword(s):  
Diabetes Mellitus ◽  
Flow Cytometry ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Type I Diabetes ◽  
Type I Diabetes Mellitus ◽  
Type I ◽  
Flow Cytometry Analysis ◽  
T Lymphocyte Subsets ◽  
Color Flow

Two-color flow cytometry analysis of activated T-lymphocyte subsets in type I diabetes mellitus

Diabetes ◽  
1988 ◽  
Vol 37 (6) ◽  
pp. 792-795 ◽  
Author(s):  
C. M. Legendre ◽  
A. Schiffrin ◽  
G. Weitzner ◽  
E. Colle ◽  
R. D. Guttmann
Keyword(s):  
Diabetes Mellitus ◽  
Flow Cytometry ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Type I Diabetes ◽  
Type I Diabetes Mellitus ◽  
Type I ◽  
Flow Cytometry Analysis ◽  
T Lymphocyte Subsets ◽  
Color Flow

T-lymphocyte subsets and thymic size in malnourished infants in Egypt: a hospital-based study

2007 ◽  
Vol 13 (5) ◽  
pp. 1031-1042 ◽  
Author(s):  
M.F. Nassar ◽  
N.T. Younis ◽  
A.G. Tohamy ◽  
D.M. Dalam ◽  
M.A. El Badawy
Keyword(s):  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
T Lymphocyte Subsets
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