scholarly journals Simultaneous flow cytometric detection of basophil activation marker CD63 and intracellular phosphorylated p38 mitogen-activated protein kinase in birch pollen allergy

2009 ◽  
Vol 76B (1) ◽  
pp. 8-17 ◽  
Author(s):  
Nicolaas E. Aerts ◽  
Evelyne J. Dombrecht ◽  
Chris H. Bridts ◽  
Margo M. Hagendorens ◽  
Luc S. de Clerck ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Birch Pollen ◽  
Pollen Allergy ◽  
Basophil Activation ◽  
Mitogen Activated Protein Kinase ◽  
Activation Marker ◽  
Flow Cytometric ◽  
Birch Pollen Allergy ◽  
Mitogen Activated Protein

The role of the inhibition of glutathione-S-transferase in the protective mechanisms of ischemic postconditioning

2013 ◽  
Vol 91 (8) ◽  
pp. 625-632 ◽  
Author(s):  
Borbála Balatonyi ◽  
Balázs Gasz ◽  
Viktória Kovács ◽  
János Lantos ◽  
Gábor Jancsó ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Annexin V ◽  
Mitogen Activated Protein Kinase ◽  
Neonatal Rat ◽  
Control Group ◽  
Glutathione S Transferase ◽  
Protective Mechanisms ◽  
Flow Cytometric ◽  
Mitogen Activated Protein ◽  
Gsk 3Β

The antioxidant glutathione-S-transferase (GST) is a crucial determinant of the development of ischaemic−reperfusion (I/R) injury, and plays a pivotal role in the regulation of the mitogen activated protein kinase (MAPK) pathways involved in stress response and apoptosis. The aim of this study was to investigate whether inhibition of GST can abolish the benefit of ischaemic postconditioning (IPoC). A neonatal rat cardiomyocyte cell culture was prepared and divided into 6 groups: (I) control group without treatment; (II) cells exposed to simulated I/R; (III) simulated I/R (sI/R) with IPoC; (IV) ethacrynic acid (EA) alone; (V) sI/R with EA; and (VI) sI/R and IPoC together with EA. Viability of the cells was measured by MTT assay, the quantity of apoptotic cells was assessed by flow cytometry following annexin V-FITC − propidium-iodide double staining. The activation of JNK, p38, ERK/p42-p44 MAPKs, and GSK-3β protein kinase was determined by flow-cytometric assay. GST inhibition markedly increased the apoptosis and decreased the cell viability despite IPoC. The protective effect of IPoC was lost in GST-inhibited groups for all MAPKs and GSK-3β. GST activity is required for the survival of cultured cardiomyocytes under stress conditions. GST inhibition was associated with differential activation of MAP and the protein kinases regulating these pathways in the process of ischaemic postconditioning.

Age-Related Changes in Activation of Mitogen-Activated Protein Kinase Cascades by Oxidative Stress

1998 ◽  
Vol 3 (1) ◽  
pp. 23-27 ◽  
Author(s):  
Kathryn Z Guyton ◽  
Myriani Gorospe ◽  
Xiantao Wang ◽  
Yolanda D Mock ◽  
Gertrude C Kokkonen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protein Kinase ◽  
Mitogen Activated Protein Kinase ◽  
Age Related ◽  
Mitogen Activated Protein ◽  
Age Related Changes
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