scholarly journals Basophil activation test by flow cytometry: Present and future applications in allergology

2008 ◽  
Vol 74B (4) ◽  
pp. 201-210 ◽  
Author(s):  
D. G. Ebo ◽  
C. H. Bridts ◽  
M. M. Hagendorens ◽  
N. E. Aerts ◽  
L. S. De Clerck ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Activation Test

Immediate allergic reaction following intra-articular injection of ioxaglate confirmed by skin testing and flow cytometry-based basophil activation test

2010 ◽  
Vol 74 (2) ◽  
pp. e27-e29 ◽  
Author(s):  
Pascale Dewachter ◽  
Samuel Castro ◽  
Frédéric Zeitoun ◽  
Sylvie Chollet-Martin ◽  
Laurence Gaillanne ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Allergic Reaction ◽  
Skin Testing ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Activation Test

Clinical implication of detecting sensitization to iodinated radiocontrast media in the basophil activation test by flow cytometry

2021 ◽  
Vol 66 (12) ◽  
pp. 747-754
Author(s):  
N. V. Bychkova ◽  
P. A. Selivanov ◽  
N. M. Kalinina
Keyword(s):  
Flow Cytometry ◽  
Immune Response ◽  
T Lymphocytes ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Activation Test ◽  
Radiocontrast Media ◽  
Type 2 Immune Response

The use of iodinated radiocontrast media is necessary for visualization. A number of patients have adverse effects of various nature and severity when these drugs are administered. Routine allergy tests do not provide adequate diagnosis of reactions to drugs in this group. The aim of this work is to assess the capabilities of the basophil activation test to confirm sensitization to non-ionic iodinated radiocontrast media, as well as to select a safe alternative drug in patients with a burdened history. Basophil activation test by flow cytometry was performed in 184 patients The Nikiforov Russian Centre of Emergency and Radiation Medicine» EMERCOM of Russia and 32 volunteers using ultravist, omnipack, and optiray. The presence of sensitization was assessed based on the basophil activation index, as well as spontaneous and anti-IgE antibody-induced activation of basophils and the population of T-lymphocytes type 2 immune response. The volunteers showed no sensitization to iodinated radiocontrast media. In patients with a medium degree of hypersensitivity reaction in vivo, in vitro sensitization to drugs was detected 4 times more often than in patients with a mild degree (51% versus 13.5%). In patients with systemic reactions to the administration of a known drug, in vitro sensitization was confirmed in 86% of cases, while the frequency of detection of sensitization to drugs did not differ. Spontaneous activation of basophils in patients and type 2 T-lymphocytes were 2 times higher than in volunteers. Patients were more likely to have low (less than 30%) activation of basophils for anti-IgE antibodies. The specificity of the basophil activation test with iodinated radiocontrast media was 100% with a sensitivity of 94.1%. Most patients were able to select a non-sensitizing contrast. Inclusion in the algorithm of spontaneous and anti-IgE antibody-induced activation of basophils and a population of T-lymphocytes type 2 immune response will allow the doctor to carry out a personalized approach to the management of patients with a burdened history.

Correspondence: Flow cytometry and basophil activation test

2003 ◽  
Vol 33 (6) ◽  
pp. 849-852 ◽  
Author(s):  
D. G. Ebo ◽  
B. Lechkar ◽  
A. J. Schuerwegh ◽  
C. H. Bridts ◽  
L. S. De Clerck ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Activation Test

Anaphylaxis to amidotrizoate proved by skin testing and flow cytometry-based basophil activation test

Allergy ◽  
2009 ◽  
Vol 64 (3) ◽  
pp. 501-502 ◽  
Author(s):  
P. Dewachter ◽  
P. Nicaise-Roland ◽  
S. Kalaboka ◽  
J. Lefèvre ◽  
S. Chollet-Martin
Keyword(s):  
Flow Cytometry ◽  
Skin Testing ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Activation Test

scholarly journals Clinical and immunological characteristics of severe bronchial asthma with Aspergillus sensitization

2021 ◽  
Vol 5 (1) ◽  
pp. 10-16
Author(s):  
Ya.I. Kozlova ◽  
◽  
E.V. Frolova ◽  
A.E. Uchevatkina ◽  
L.V. Filippova ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Bronchial Asthma ◽  
Stimulation Index ◽  
Basophil Activation Test ◽  
Specific Ige ◽  
Forced Expiratory Volume ◽  
Basophil Activation ◽  
Activation Test ◽  
To Receive

Aim: to assess the clinical and immunological characteristics of patients with severe bronchial asthma (BA) with Aspergillus sensitization and to study the possibilities of applying basophil activation test (BAT) using flow cytometry to detect mycogenic sensitization. Patients and Methods: 93 patients with severe BA were examined. Therapy had 4 or 5 steps according to GINA 2019 recommendations. TARC (thymus and activation-regulated chemokine) content, the number of eosinophils, the levels of total IgE and specific IgE to 10 allergens were determined in blood serum by enzyme immunoassay. BAT with Aspergillus fumigatus allergen was performed in vitro using flow cytometry. Results: Aspergillus sensitization was detected in 33 (35.4%) patients with severe BA. In patients with BA and Aspergillus sensitization, the disease course was uncontrolled, and patients in this group were credibly more likely to receive oral glucocorticosteroids. The number of basophils (activated by the A. fumigatus allergen) and the stimulation index in patients with BA and Aspergillus sensitization were significantly higher than in patients with BA (9.9 (6.0–24.0) % vs. 3.6 (2.0–5.4) %; (p=0.000) and 4.25 (2.49–9.30) vs. 0.94 (0.75–1.16); (p=0.000)). Significant differences in TARC content were obtained in the groups of patients with severe BA and Aspergillus sensitization and patients with BA (625.0 (418.4–875.0) pg/mg versus 406.0 (210.0–561.0) pg/mg; p=0.001). A negative correlation was determined between TARC levels and a decrease in forced expiratory volume in 1 second (FEV1) (r=-0.70, p<0.05), and between positive correlation and absolute eosinophil count (r=0.81, p<0.05) and level of specific IgE to Aspergillus (r=0.36, p<0.05). Conclusion: Aspergillus sensitization is associated with an uncontrolled BA course. An additional method for diagnosing mycogenic sensitization is the BAT. The TARC concentration can serve as a biomarker of an active inflammatory response. KEYWORDS: Aspergillus spp., severe bronchial asthma, mycogenic sensitization, basophil activation test, TARC. FOR CITATION: Kozlova Ya.I., Frolova E.V., Uchevatkina A.E. et al. Clinical and immunological characteristics of severe bronchial asthma with Aspergillus sensitization. Russian Medical Inquiry. 2021;5(1):10–16. DOI: 10.32364/2587-6821-2021-5-1-10-16.

scholarly journals New opportunities in the diagnosis of asthma with sensitization to Aspergillus spp.

2021 ◽  
Vol 13 (2) ◽  
pp. 67-76
Author(s):  
Yana I. Kozlova ◽  
Alexandra E. Uchevatkina ◽  
Larisa V. Filippova ◽  
Oleg V. Aak ◽  
Valeriy D. Kuznetsov ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Stimulation Index ◽  
Allergic Bronchopulmonary Aspergillosis ◽  
Basophil Activation Test ◽  
Study Groups ◽  
Specific Ige ◽  
Basophil Activation ◽  
Activation Test ◽  
Cellular Analysis ◽  
Index Value

BACKGROUND:Diagnosis of asthma with sensitization toAspergillusspp. is becoming increasingly important due to the severe, uncontrolled course of the disease and the possibility of the formation of allergic bronchopulmonary aspergillosis. AIM:To evaluate the possibility of using the basophil activation test using flow cytometry for the diagnosis of asthma with sensitization toAspergillusspp. MATERIALS AND METHODS:118 patients with asthma were examined. The levels of total IgE and specific IgE to aeroallergens were determined in the blood serum by the enzyme immunoassay. Basophil activation was studied by flow cytometry using the Allergenicity kit (Cellular Analysis of Allergy, Beckman-Coulter, USA). The allergenAspergillus fumigatus(Alcor Bio, Russia) was used to stimulate basophils. RESULTS:The first group consisted of 57 patients with asthma without sensitization toAspergillusspp. The second group included 36 patients with asthma with sensitization toAspergillusspp. The third group consisted of 25 patients with allergic bronchopulmonary aspergillosis. The number of basophils activated by theAspergillusfumigatusallergen in patients with asthma with sensitization toAspergillusspp. and allergic bronchopulmonary aspergillosis was significantly higher than in the asthma group and amounted to 8.1 [5.2; 20.9]% and 84.6 [75.7; 94.0]%, respectively (p 0.001). The stimulation index in the study groups ranged from 0.7 to 72.6. The optimal diagnostic point (cut off) for identifying patients with asthma withAspergillusspp. sensitization there was an stimulation index value of more than 2.4, and for patients with allergic bronchopulmonary aspergillosis 15.95. Among all patients with sensitization toAspergillusspp. a positive correlation was established between the level of specific IgE toAspergillusspp. and the percentage of basophils activated by the allergenAspergillusfumigatus(r= 0.792,p 0.001) and stimulation index (r= 0.796,p 0.05). CONCLUSIONS:The basophil activation test can be used as an additional diagnostic method for asthma with sensitization toAspergillusspp. and allergic bronchopulmonary aspergillosis.

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