scholarly journals Flow cytometric immunphenotyping of epithelial cancer cells in effusions—Technical considerations and pitfalls

2007 ◽  
Vol 72B (5) ◽  
pp. 332-343 ◽  
Author(s):  
Hiep P. Dong ◽  
Arild Holth ◽  
Aasmund Berner ◽  
Ben Davidson ◽  
Björn Risberg
Keyword(s):  
Cancer Cells ◽  
Epithelial Cancer ◽  
Flow Cytometric ◽  
Technical Considerations

scholarly journals Response to inhibition of smoothened in diverse epithelial cancer cells that lack smoothened or patched 1 mutations

2012 ◽  
Vol 41 (5) ◽  
pp. 1751-1761 ◽  
Author(s):  
FABRIZIO GALIMBERTI ◽  
ALEXANDER M. BUSCH ◽  
FADZAI CHINYENGETERE ◽  
TIAN MA ◽  
DAVID SEKULA ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Epithelial Cancer ◽  
Patched 1

scholarly journals Oxidation of Ovarian Epithelial Cancer Cells by Hypochlorous Acid Enhances Immunogenicity and Stimulates T Cells that Recognize Autologous Primary Tumor

2008 ◽  
Vol 14 (15) ◽  
pp. 4898-4907 ◽  
Author(s):  
Cheryl L-L. Chiang ◽  
Jonathan A. Ledermann ◽  
Egla Aitkens ◽  
Elizabeth Benjamin ◽  
David R. Katz ◽  
...  
Keyword(s):  
T Cells ◽  
Cancer Cells ◽  
Primary Tumor ◽  
Hypochlorous Acid ◽  
Epithelial Cancer ◽  
Ovarian Epithelial Cancer

scholarly journals P11.41 Comparison of fibroblast activation protein expression and localization in glioblastomas and brain metastases

2019 ◽  
Vol 21 (Supplement_3) ◽  
pp. iii52-iii52
Author(s):  
P Busek ◽  
M Zubal ◽  
B Chmielova ◽  
Z Vanickova ◽  
P Hrabal ◽  
...  
Keyword(s):  
Tumor Microenvironment ◽  
Brain Metastases ◽  
Cancer Cells ◽  
Brain Tissue ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Glioma Cells ◽  
Fibroblast Activation Protein ◽  
Epithelial Cancer ◽  
Fibroblast Activation

Abstract BACKGROUND Fibroblast activation protein (FAP) is a transmembrane serine protease that is frequently upregulated in the tumor microenvironment. In several cases, FAP protein itself and/or FAP expressing stromal cells have been shown to contribute to cancer progression and to be associated with more aggressive cancer behaviour and shorter patient survival. The aim of this study was to determine FAP expression in glioblastomas and brain metastases and to identify the cell types that express FAP in the microenvironment of these malignancies. MATERIAL AND METHODS FAP enzymatic activity and protein concentration were determined in samples from patients with brain metastases, glioblastomas and pharmacoresistant epilepsy (control non-tumorous brain tissue) by an enzymatic assay using a specific fluorogenic substrate and ELISA, respectively. Immunohistochemical labelling with antibodies against FAP and markers of astroglia, epithelial cancer cells and mesenchymal stromal cells was performed to characterize FAP expressing cells. RESULTS FAP was significantly upregulated in the majority of glioblastomas and brain metastases in comparison to non-tumorous brain tissue. In glioblastomas, FAP was localized perivascularly and in mesenchymal cells, and in part of the tumors also in the glioma cells. In brain metastases, FAP positivity was abundantly present in the stroma and predominantly co-localised with markers of mesenchymal stromal cells (TE-7, SMA, PDGFRbeta, NG2), but there was no overlap between FAP and markers of epithelial cancer cells (EpCAM, pancytokeratin). CONCLUSION FAP is upregulated in the microenvironment of human glioblastomas and brain metastases compared to non-tumorous brain tissue. In glioblastomas, FAP is expressed in part of the glioma cells, in pericytes and mesenchymal stromal cells, whereas no positivity in cancer cells and more abundant FAP+ stroma was detected in brain metastases. The selective expression of FAP in these brain tumors may be useful for the visualization and possibly therapeutic targeting of their tumor microenvironment. GRANT SUPPORT Ministry of Health of the Czech Republic, grant No. 15-31379A, Progres Q28/LF1, 2015064 LM EATRIS and the project,Center for Tumor Ecology - Research of the Cancer Microenvironment Supporting Cancer Growth and Spread” (reg. n. CZ.02.1.01/0.0/0.0/16_019/0000785) supported by the Operational Programme Research, Development and Education.

A proteomic analysis of differential cellular responses to the short-chain fatty acids butyrate, valerate and propionate in colon epithelial cancer cells

2012 ◽  
Vol 8 (4) ◽  
pp. 1146-1156 ◽  
Author(s):  
Josephine Kilner ◽  
Jennifer S. Waby ◽  
Joanna Chowdry ◽  
Abdul Q. Khan ◽  
Josselin Noirel ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Cancer Cells ◽  
Proteomic Analysis ◽  
Cellular Responses ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Epithelial Cancer ◽  
Chain Fatty Acids

scholarly journals Epigenetic regulation (DNA methylation, histone modifications) of the 11p15 mucin genes (MUC2, MUC5AC, MUC5B, MUC6) in epithelial cancer cells

Oncogene ◽  
2007 ◽  
Vol 26 (45) ◽  
pp. 6566-6576 ◽  
Author(s):  
A Vincent ◽  
M Perrais ◽  
J-L Desseyn ◽  
J-P Aubert ◽  
P Pigny ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Cancer Cells ◽  
Histone Modifications ◽  
Epigenetic Regulation ◽  
Epithelial Cancer ◽  
Mucin Genes

scholarly journals Efficient and simple approach to in vitro culture of primary epithelial cancer cells

2016 ◽  
Vol 36 (6) ◽  
Author(s):  
Karolina Janik ◽  
Marta Popeda ◽  
Joanna Peciak ◽  
Kamila Rosiak ◽  
Maciej Smolarz ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
In Vitro Culture ◽  
Cancer Cells ◽  
Simple Approach ◽  
Primary Cell ◽  
Epithelial Cancer ◽  
Extracellular Matrix Components ◽  
Matrix Components ◽  
Cell Medium

Primary breast and prostate epithelial cancer cells may be efficiently cultured in vitro using simple and easily validatable approach–plates coated with a mixture of extracellular matrix components and tissue-specific primary cell medium.

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