scholarly journals Guidelines for improving the reproducibility of quantitative multiparameter immunofluorescence measurements by laser scanning cytometry on fixed cell suspensions from human solid tumors

2005 ◽  
Vol 70B (1) ◽  
pp. 10-19 ◽  
Author(s):  
Stanley Shackney ◽  
David R. Emlet ◽  
Agnese Pollice ◽  
Charles Smith ◽  
Kathryn Brown ◽  
...  
Keyword(s):  
Solid Tumors ◽  
Laser Scanning ◽  
Cell Suspensions ◽  
Laser Scanning Cytometry ◽  
Human Solid Tumors ◽  
Fixed Cell

scholarly journals A suitable method for identifying cell aggregates in laser scanning cytometry listmode data for analyzing disaggregated cell suspensions obtained from human cancers

Cytometry ◽  
2004 ◽  
Vol 59B (1) ◽  
pp. 10-23 ◽  
Author(s):  
Stanley E. Shackney ◽  
Charles A. Smith ◽  
Agnese A. Pollice ◽  
Kathryn Brown ◽  
Deborah Kosiban
Keyword(s):  
Laser Scanning ◽  
Cell Suspensions ◽  
Suitable Method ◽  
Cell Aggregates ◽  
Human Cancers ◽  
Laser Scanning Cytometry

Laser Scanning Cytometry in Pathology of Solid Tumors

1997 ◽  
Vol 41 (1) ◽  
pp. 98-108 ◽  
Author(s):  
Wojciech Gorczyca ◽  
Zbigniew Darzynkiewicz ◽  
Myron R. Melamed
Keyword(s):  
Solid Tumors ◽  
Laser Scanning ◽  
Laser Scanning Cytometry

Enumeration of Cryptosporidium oocysts from surface water concentrates by laser-scanning cytometry

2000 ◽  
Vol 41 (7) ◽  
pp. 197-202 ◽  
Author(s):  
F. Zanelli ◽  
B. Compagnon ◽  
J. C. Joret ◽  
M. R. de Roubin
Keyword(s):  
Surface Water ◽  
Water Samples ◽  
Laser Scanning ◽  
Immunomagnetic Separation ◽  
Entire Surface ◽  
Cryptosporidium Oocysts ◽  
Different Types ◽  
Laser Scanning Cytometry ◽  
Direct Microscopic Observation

The utilization of the ChemScan® RDI was tested for different types of water concentrates. Concentrates were prepared by cartridge filtration or flocculation, and analysed either without purification, or after Immunomagnetic separation (IMS) or flotation on percoll-sucrose gradients. Theenumeration of the oocysts was subsequently performed using the ChemScan® RDI Cryptosporidium application. Enumeration by direct microscopic observation of the entire surface of the membrane was carried out as a control, and recoveries were calculated as a ratio between the ChemScan® RDI result and the result obtained with direct microscopic enumeration. The Chemscan enumeration technique proved reliable, with recoveries yielding close to 100% in most cases (average 125%, range from 86 to 467%) for all the concentration/purification techniques tested. The quality of the antibodies was shown to be critical, with antibodies from some suppliers yielding recoveries a low as 10% in some cases. This difficulty could, however, be overcome by the utilization of the antibody provided by Chemunex. These data conclusively prove that laser scanning cytometry, which greatly facilitates the microscopic enumeration of Cryptosporidium oocysts from water samples and decreases the time of observation by four to six times, can be successfully applied to water concentrates prepared from a variety of concentration/purification techniques.

scholarly journals Large hypomethylated blocks as a universal defining epigenetic alteration in human solid tumors

2014 ◽  
Vol 6 (8) ◽  
Author(s):  
Winston Timp ◽  
Hector Corrada Bravo ◽  
Oliver G McDonald ◽  
Michael Goggins ◽  
Chris Umbricht ◽  
...  
Keyword(s):  
Solid Tumors ◽  
Epigenetic Alteration ◽  
Human Solid Tumors
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