Monitoring radiofrequency therapy‐induced tumor cell dissemination by in vivo flow cytometry

2021 ◽  
Author(s):  
Xi Zhu ◽  
Cong Wei ◽  
Yang Zhang ◽  
Zheying Meng ◽  
Bing Hu ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Tumor Cell ◽  
Tumor Cell Dissemination ◽  
Radiofrequency Therapy ◽  
In Vivo Flow Cytometry ◽  
Cell Dissemination

scholarly journals Real-time monitoring of circulating tumor cell (CTC) release after nanodrug or tumor radiotherapy using in vivo flow cytometry

2017 ◽  
Vol 492 (3) ◽  
pp. 507-512 ◽  
Author(s):  
Nathan A. Koonce ◽  
Mazen A. Juratli ◽  
Chengzhong Cai ◽  
Mustafa Sarimollaoglu ◽  
Yulian A. Menyaev ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Tumor Cell ◽  
Real Time ◽  
Circulating Tumor Cell ◽  
Real Time Monitoring ◽  
In Vivo Flow Cytometry

scholarly journals The notch target gene HEYL modulates metastasis forming capacity of colorectal cancer patient-derived spheroid cells in vivo

BMC Cancer ◽  
2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Sarah Weber ◽  
Sebastian E. Koschade ◽  
Christopher M. Hoffmann ◽  
Taronish D. Dubash ◽  
Klara M. Giessler ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Tumor Cell ◽  
Target Gene ◽  
Colorectal Cancer Patient ◽  
Notch Pathway ◽  
Tumor Cell Dissemination ◽  
Metastasis Formation ◽  
Cell Dissemination ◽  
The Impact

Abstract Background While colorectal cancer (CRC) patients with localized disease have a favorable prognosis, the five-year-survival rate in patients with distant spread is still below 15%. Hence, a detailed understanding of the mechanisms regulating metastasis formation is essential to develop therapeutic strategies targeting metastasized CRC. The notch pathway has been shown to be involved in the metastatic spread of various tumor entities; however, the impact of its target gene HEYL remains unclear so far. Methods In this study, we functionally assessed the association between high HEYL expression and metastasis formation in human CRC. Therefore, we lentivirally overexpressed HEYL in two human patient-derived CRC cultures differing in their spontaneous metastasizing capacity and analyzed metastasis formation as well as tumor cell dissemination into the bone marrow after xenotransplantation into NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice. Results HEYL overexpression decreased tumor cell dissemination and the absolute numbers of formed metastases in a sub-renal capsular spontaneous metastasis formation model, addressing all steps of the metastatic cascade. In contrast, metastatic capacity was not decreased following intrasplenic xenotransplantation where the cells are placed directly into the blood circulation. Conclusion These results suggest that HEYL negatively regulates metastasis formation in vivo presumably by inhibiting intravasation of metastasis-initiating cells.

scholarly journals Conditional ROCK Activation In vivo Induces Tumor Cell Dissemination and Angiogenesis

2004 ◽  
Vol 64 (24) ◽  
pp. 8994-9001 ◽  
Author(s):  
Daniel R. Croft ◽  
Erik Sahai ◽  
Georgia Mavria ◽  
Shuixing Li ◽  
Jeff Tsai ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Tumor Cell Dissemination ◽  
Cell Dissemination

Real-time monitoring of circulating tumor cell release during tumor manipulation using in vivo photoacoustic and fluorescent flow cytometry

Head & Neck ◽  
2013 ◽  
Vol 36 (8) ◽  
pp. 1207-1215 ◽  
Author(s):  
Mazen A. Juratli ◽  
Mustafa Sarimollaoglu ◽  
Eric R. Siegel ◽  
Dmitry A. Nedosekin ◽  
Ekaterina I. Galanzha ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Tumor Cell ◽  
Real Time ◽  
Circulating Tumor Cell ◽  
Real Time Monitoring ◽  
Cell Release
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