scholarly journals OMIP 073: Analysis of human thymocyte development with a 14‐color flow cytometry panel

2021 ◽  
Author(s):  
Sarah‐Jolan Bremer ◽  
Laura Glau ◽  
Christina Gehbauer ◽  
Annika Boxnick ◽  
Daniel Biermann ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Thymocyte Development ◽  
Color Flow ◽  
Human Thymocyte

Immunophenotyping of T Lymphocytes by Three-Color Flow Cytometry in Healthy Newborns, Children, and Adults

1997 ◽  
Vol 84 (1) ◽  
pp. 46-55 ◽  
Author(s):  
Thomas W. McCloskey ◽  
Terri Cavaliere ◽  
Saroj Bakshi ◽  
Rita Harper ◽  
James Fagin ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Lymphocytes ◽  
Color Flow

scholarly journals Standardization of 8-color flow cytometry across different flow cytometer instruments: A feasibility study in clinical laboratories in Switzerland

2019 ◽  
Vol 475 ◽  
pp. 112348 ◽  
Author(s):  
Hana Glier ◽  
Ingmar Heijnen ◽  
Mathieu Hauwel ◽  
Jan Dirks ◽  
Stéphane Quarroz ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Feasibility Study ◽  
Flow Cytometer ◽  
Color Flow ◽  
Clinical Laboratories

Reduction of variation in T-cell subset enumeration among 55 laboratories using single-platform, three or four-color flow cytometry based on CD45 and SSC-based gating of lymphocytes

Cytometry ◽  
2002 ◽  
Vol 50 (2) ◽  
pp. 92-101 ◽  
Author(s):  
Jan W. Gratama ◽  
Jaco Kraan ◽  
Mike Keeney ◽  
Viv Granger ◽  
David Barnett
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
Cell Subset ◽  
Color Flow ◽  
T Cell Subset

Immunophenotypic Study of Basophils by Multiparameter Flow Cytometry

2008 ◽  
Vol 132 (5) ◽  
pp. 813-819
Author(s):  
Xiaohong Han ◽  
Jeffrey L. Jorgensen ◽  
Archana Brahmandam ◽  
Ellen Schlette ◽  
Yang O. Huh ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
Chronic Myelogenous Leukemia ◽  
Peripheral Blood ◽  
Myelogenous Leukemia ◽  
Multiparameter Flow Cytometry ◽  
Color Flow ◽  
Aberrant Expression ◽  
Flow Cytometric ◽  
Hla Dr

Abstract Context.—The immunophenotypic profile of basophils is not yet fully established, and the immunophenotypic changes in chronic myelogenous leukemia are not fully characterized. Objective.—To establish a comprehensive immunophenotypic spectrum of normal basophils and to assess the range of immunophenotypic aberrations of basophils in chronic myelogenous leukemia. Design.—Using 4-color flow cytometry, we compared the immunophenotypic profile of basophils in peripheral blood or bone marrow samples from 20 patients with no evidence of neoplasia to basophils from 15 patients with chronic myelogenous leukemia. Results.—Basophils in control cases were all positive for CD9, CD13, CD22, CD25 (dim), CD33, CD36, CD38 (bright), CD45 (dimmer than lymphocytes and brighter than myeloblasts), and CD123 (bright), and were negative for CD19, CD34, CD64, CD117, and HLA-DR. Basophils in all chronic myelogenous leukemia patients possessed 1 to 5 immunophenotypic aberrancies. The most common aberrancies were underexpression of CD38, followed by aberrant expression of CD64 and underexpression of CD123. CD34 and CD117 were present in cases with basophilic precursors. Myeloblasts showed a distinct immunophenotypic profile, as they typically expressed CD34 and CD117, showed dimmer expression (compared with basophils) of CD38, CD45, and CD123, and lacked expression of CD22. Conclusions.—Flow cytometric immunophenotyping can identify immunophenotypic aberrations of basophils in chronic myelogenous leukemia, and discriminate basophils from myeloblasts.

Heterogeneous calcium flux in peripheral T cell subsets revealed by five-color flow cytometry using log-ratio circuitry

Cytometry ◽  
1995 ◽  
Vol 21 (2) ◽  
pp. 187-196 ◽  
Author(s):  
M. Roederer ◽  
M. Bigos ◽  
T. Nozaki ◽  
R. T. Stovel ◽  
D. R. Parks ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
T Cell Subsets ◽  
Calcium Flux ◽  
Color Flow ◽  
Log Ratio

scholarly journals Recombinant human interleukin-11 stimulates megakaryocytopoiesis and increases peripheral platelets in normal and splenectomized mice

Blood ◽  
1993 ◽  
Vol 81 (4) ◽  
pp. 901-908 ◽  
Author(s):  
TY Neben ◽  
J Loebelenz ◽  
L Hayes ◽  
K McCarthy ◽  
J Stoudemire ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
Color Flow ◽  
Platelet Counts ◽  
In Vivo Treatment ◽  
Interleukin 11 ◽  
Megakaryocyte Progenitors

Abstract The effects of recombinant human interleukin-11 (rhIL-11) on in vivo mouse megakaryocytopoeisis were examined. Normal C57Bl/6 mice and splenectomized C57Bl/6 mice were treated for 7 days with 150 micrograms/kg rhIL-11 administered subcutaneously. In normal mice, peripheral platelet counts were elevated compared with vehicle-treated controls after 3 days of rhIL-11 treatment and remained elevated until day 10. Splenectomized mice treated with rhIL-11 showed elevated peripheral platelet counts that were similar in magnitude to normal rhIL-11-treated mice. However, on day 10 the platelet counts in rhIL-11- treated, splenectomized mice were no longer elevated. Analysis of bone marrow megakaryocyte ploidy by two-color flow cytometry showed an increase, relative to controls, in the percentage of 32N megakaryocytes in both normal and splenectomized animals treated with rhIL-11. In normal mice, the number of spleen megakaryocyte colony-forming cells (MEG-CFC) were increased twofold to threefold relative to controls after 3 and 7 days of rhIL-11 treatment, whereas the number of bone marrow MEG-CFC were increased only on day 7. The number of MEG-CFC in the bone marrow of rhIL-11-treated, splenectomized mice was increased twofold compared with controls on both days 3 and 7 of the study. These data show that in vivo treatment of normal or splenectomized mice with rhIL-11 increased megakaryocyte progenitors, stimulated endoreplication of bone marrow megakaryocytes, and increased peripheral platelet counts. In addition, results in splenectomized mice showed that splenic hematopoiesis was not essential for the observed increases in peripheral platelets in response to rhIL-11 administration.

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