scholarly journals Improved Flow Cytometric Light Scatter Detection of Submicron‐Sized Particles by Reduction of Optical Background Signals

2020 ◽  
Vol 97 (6) ◽  
pp. 610-619
Author(s):  
Ger J. A. Arkesteijn ◽  
Estefanía Lozano‐Andrés ◽  
Sten F. W. M. Libregts ◽  
Marca H. M. Wauben
Keyword(s):  
Light Scatter ◽  
Flow Cytometric ◽  
Optical Background

Flow cytometric enumeration of absolute lymphocyte number in peripheral blood using two parameters of light scatter

Cytometry ◽  
1985 ◽  
Vol 6 (2) ◽  
pp. 172-174 ◽  
Author(s):  
Y. C. Smart ◽  
J. Cox ◽  
B. Murphy ◽  
A. Enno ◽  
R. C. Burton
Keyword(s):  
Peripheral Blood ◽  
Light Scatter ◽  
Flow Cytometric ◽  
Two Parameters ◽  
Lymphocyte Number

scholarly journals Electrolytic degradation of DNA fluorochromes during flow cytometric measurement of electronic cell volume.

1980 ◽  
Vol 28 (4) ◽  
pp. 330-334 ◽  
Author(s):  
O Alabaster ◽  
D L Glaubiger ◽  
V T Hamilton ◽  
S A Bentley ◽  
S E Shackney ◽  
...  
Keyword(s):  
Cell Volume ◽  
Dna Content ◽  
Chemical Degradation ◽  
Quantum Yields ◽  
Light Scatter ◽  
Electrolytic Production ◽  
Flow Cytometric ◽  
Chlorine Gas ◽  
Electrode Polarity ◽  
Flow Cytometric Measurement

Changes in flow cytometric measurement of DNA content can result from electrolytic chemical degradation of mithramycin, ethidium bromide, and propidium iodide during simultaneous measurement of electronic cell volume. Bench electrolysis also degrades these fluorochromes without changing the quantum yields, even when they are complexed to DNA. In the flow cytometer, electrolytic production of chlorine at the anode is the probable cause of this degradation, since exposure of these fluorochromes to chlorine gas produces the same effect. It is therefore advisable to measure the DNA content distribution alone before simultaneously measuring the DNA content and the electronic cell volume. If unavoidable effects on the DNA distribution are present, narrow forward-angle light scatter should be used as the cell size indicator during dual parameter measurements. Modifying instrument design by reversing electrode polarity might eliminate this problem.

Diagnostic Utility of Flow Cytometry in Myelodysplastic Syndrome: A Retrospective Validation From a Single Centre.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 4846-4846
Author(s):  
Pervin Topcuoglu ◽  
Klara Dalva ◽  
Sule Mine Bakanay ◽  
Sinem Civriz Bozdag ◽  
Onder Arslan ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
Myelodysplastic Syndrome ◽  
Antigen Expression ◽  
Pathological Examination ◽  
Light Scatter ◽  
Myeloid Lineage ◽  
Hematopoietic Stem ◽  
Altered Expression ◽  
Flow Cytometric

Abstract Abstract 4846 Myelodysplastic syndrome (MDS) is heterogeneous clonal hematopoietic stem cell disorder characterized by cytopenia(s) and dysplasia in one or more cell lineage. Though flow cytometry (FCM) is an important diagnostic tool in hematopoietic cell disorders, a prominent immunophenotyping feature in MDS may not be determined. In this study, we retrospectively evaluated flow cytometric features of bone marrow samples diagnosed as MDS with clinical and hematological findings. Patients-Method Between Feb 2004 and March 2009, flow cytometric parameters of 73 patients (M/F: 50/23) with MDS were re-analyzed. Median age was 59 years (17-89 ys). Our general principles are to evaluate quality of bone marrow samples, to determine proportion of the cells and features of their light scatter, and to give percentage of the blast. When detected a finding of dysplasia in the first analysis, the second step includes the determination of the maturation of the cells and the presence of the aberrant antigen expression. Results The samples were interpreted as MDS in % 76.7, MDS-RAEB-1 or RAEB-2 in %16.4, myeloproliferative disorder in %1.4 and non-diagnostic in %6.8 of the cases by flow cytometric examination. We detected variable degrees of hypogranulation in myeloid lineage in %82.2 of the samples by the light scatter features of the cells: 85% of severe and 15% of moderate or mild hypogranulation. The ratio of myeloid and lymphoid was changing from 0.3 to 17.5 (median 2). The decreasing of this ratio (<1) was observed in 19.4% of the samples. We detected altered expression of mature granulocyte. These included decreasing or lack of expression in CD15 45/73 (61.4%), CD13 38/70 (54.3%), CD16 53/67 (79.1%), CD11b 51/71 (71.8%), CD24 44/69 (65.2%), CD10 23/72 (31.9%) and MPO 14/72 (19.4%). Besides, bright expression of CD33 in 53.5% of the samples was observed. CD36 and CD56 in myeloid lineage were co-expressed in about 50 % of the samples. In 80.8 % of the samples dysplasia in erythroid compartment could be evaluated: Expression of CD71 according to glycophorin A (ratio <1) was decreased in 23.7 %. When we made similar analysis in the samples without RAEB-1 and -2 as pathological examination of bone marrow, 13.4 % of the samples could not be evaluated in favor of dysplasia. Of the samples with dysplasia hypogranulation, aberrant antigen expression of myeloid lineage and eryhtroid dysplasia were observed in 92.1%, 34.1% and 31.5%, respectively. In conclusion, FCM events may help to the differantial diagnosis of MDS especially when combining with clinical events. Improving of the analysis by focusing on the blast characteristics may be a standard approach to evaluate for low risk MDS. Disclosures No relevant conflicts of interest to declare.

scholarly journals The malignant cells in a Lennert's lymphoma are T lymphocytes with a mature helper surface phenotype. A multiparameter flow cytometric analysis

Blood ◽  
1986 ◽  
Vol 68 (2) ◽  
pp. 426-429 ◽  
Author(s):  
KJ Stonesifer ◽  
NA Benson ◽  
SE Ryden ◽  
DF Pawliger ◽  
RC Braylan
Keyword(s):  
Flow Cytometry ◽  
Flow Cytometric Analysis ◽  
Malignant Neoplasm ◽  
Surface Antigens ◽  
Light Scatter ◽  
Malignant Cells ◽  
T Helper ◽  
T Helper Lymphocytes ◽  
Flow Cytometric ◽  
Multiparameter Analysis

Abstract The flow cytometric analysis of DNA content in cells obtained from a case of Lennert's lymphoma demonstrated the presence of a discrete hypotetraploid cell population. Correlated multiparameter analysis of DNA, light scatter, and surface antigens by flow cytometry showed that the hypotetraploid cells were intermediate to large cells expressing T11, T3, and T4 antigens and lacking B1 and T8 antigens. These findings suggest that Lennert's lymphoma represents a malignant neoplasm of T- helper lymphocytes.

scholarly journals Flow cytometric analysis of hairy cell leukemia using right-angle light scatter

Cytometry ◽  
1986 ◽  
Vol 7 (2) ◽  
pp. 217-220 ◽  
Author(s):  
Dirk R. Van Bockstaele ◽  
Zwi N. Berneman ◽  
Marc E. Peetermans
Keyword(s):  
Hairy Cell Leukemia ◽  
Flow Cytometric Analysis ◽  
Light Scatter ◽  
Hairy Cell ◽  
Cell Leukemia ◽  
Flow Cytometric
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