scholarly journals International Society for Advancement of Cytometry (ISAC) flow cytometry shared resource laboratory (SRL) best practices

2016 ◽  
Vol 89 (11) ◽  
pp. 1017-1030 ◽  
Author(s):  
Lora W. Barsky ◽  
Michele Black ◽  
Matthew Cochran ◽  
Benjamin J. Daniel ◽  
Derek Davies ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Best Practices ◽  
International Society ◽  
Shared Resource ◽  
Resource Laboratory

Considerations for User Consultation in a Flow Cytometry Shared Resource Laboratory

2021 ◽  
Author(s):  
Amy Graham ◽  
Jena Korecky ◽  
Eric Schultz ◽  
Michael Gregory ◽  
Kewal Asosingh
Keyword(s):  
Flow Cytometry ◽  
Shared Resource ◽  
Resource Laboratory

scholarly journals Adapting to the Coronavirus Pandemic: Building and Incorporating a Diagnostic Pipeline in a Shared Resource Laboratory

2020 ◽  
Author(s):  
Emma Russell ◽  
Ana Agua‐Doce ◽  
Lotte Carr ◽  
Asha Malla ◽  
Kerol Bartolovic ◽  
...  
Keyword(s):  
Shared Resource ◽  
Resource Laboratory

scholarly journals How Should We Be Conducting Routine Analysis of Traditional Emergency Department Syndromic Surveillance Data?

2015 ◽  
Vol 7 (1) ◽  
Author(s):  
David Atrubin ◽  
Michael Wiese
Keyword(s):  
Emergency Department ◽  
Best Practices ◽  
International Society ◽  
Disease Surveillance ◽  
Syndromic Surveillance ◽  
Surveillance Data ◽  
Outbreak Detection ◽  
Surveillance Systems ◽  
Track Record ◽  
Periodic Disease

This roundtable will focus on how traditional emergency department syndromic surveillance systems should be used to conduct daily or periodic disease surveillance.  As outbreak detection using these systems has demonstrated an equivocal track record, epidemiologists have sought out other interesting uses for these systems.  Over the numerous years of the International Society for Disease Surveillance (ISDS) Conference, many of these studies have been presented; however, there has been a dearth of discussion related to how these systems should be used. This roundtable offers a forum to discuss best practices for the routine use of emergency department syndromic surveillance data.

Best Practices for Authentication of Cell Lines to Ensure Data Reproducibility and Integrity

2021 ◽  
Author(s):  
Elisabeth Vicente ◽  
Megan Lesniewski ◽  
Diana Newman ◽  
Zeljko Vujaskovic ◽  
Isabel L. Jackson
Keyword(s):  
Flow Cytometry ◽  
Best Practices ◽  
Cell Line ◽  
Dna Barcoding ◽  
Cell Lines ◽  
Cell Identity ◽  
Purity Analysis ◽  
Primary Rabbit ◽  
Commercial Vendor ◽  
Aortic Endothelial

Cell line misidentification and contamination are major contributors to the reproducibility crisis in academic research. Authentication of cell lines provides assurances of the data generated; however, commercially available cells are often not subjected to rigorous identification testing. In this study, commercially available cell lines underwent testing to confirm cell identity and purity. The methods reported here outline the best practices for cell line authentication. Briefly, a commercially available primary rabbit aortic endothelial cell line was purchased for the intent of producing target proteins necessary for generating species-specific recombinant antibodies. These rabbit-specific antibodies would then be utilized for the development of in-house enzyme-linked immunosorbent assays (ELISA) to evaluate blood-based biomarkers of vascular injury after total-body irradiation. To authenticate the cell line, cell identity and purity were determined by single tandem repeat (STR) testing, flow cytometry, polymerase chain reaction (PCR), and cytochrome c oxidase subunit 1 (CO1) DNA Barcoding in-house and/or through commercial vendors. Fresh cells obtained from a New Zealand White rabbit (Charles River, Wilmington, DE) were used as a positive control. The results of STR and flow cytometry analyses indicated the cells were not contaminated with human or mouse cells, and that the cells were not of endothelial origin. PCR demonstrated that cells were also not of rabbit origin, which was further confirmed by a third-party vendor. An unopened vial of cells was submitted to another vendor for CO1 DNA Barcoding analysis, which identified the cells as being purely of bovine origin. Results revealed that despite purchase through a commercial vendor, the cell line marketed as primary rabbit aortic endothelial cells were of bovine origin. Purity analysis found cells were misidentified rather than contaminated. Further investigation to determine the cell type was not performed. The most cost-effective and efficient methodology for confirming cell line identity was found to be CO1 DNA Barcoding performed by a commercial vendor.

Best practices in the flow cytometry of microalgae

2021 ◽  
Vol 99 (4) ◽  
pp. 359-364
Author(s):  
Dora Čertnerová ◽  
David W. Galbraith
Keyword(s):  
Flow Cytometry ◽  
Best Practices

Application‐based guidelines for best practices in plant flow cytometry

2021 ◽  
Author(s):  
Elwira Sliwinska ◽  
João Loureiro ◽  
Ilia J. Leitch ◽  
Petr Šmarda ◽  
Jillian Bainard ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Best Practices
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