scholarly journals Glioblastoma-mesenchymal stem cell communication modulates expression patterns of kinin receptors: Possible involvement of bradykinin in information flow

2015 ◽  
Vol 89 (4) ◽  
pp. 365-375 ◽  
Author(s):  
Micheli M. Pillat ◽  
Mona N. Oliveira ◽  
Helena Motaln ◽  
Barbara Breznik ◽  
Talita Glaser ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Information Flow ◽  
Expression Patterns ◽  
Cell Communication ◽  
Kinin Receptors

Umbilical Mesenchymal Stem Cell-Derived Extracellular Vesicle Conditioning Has an Immunosuppressive Effect on NK Cells

2021 ◽  
Author(s):  
G. Dostert ◽  
V. Jouan-Hureaux ◽  
H. Louis ◽  
É. Velot
Keyword(s):  
Flow Cytometry ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Nk Cells ◽  
Cell Activation ◽  
Nk Cell ◽  
Cell Communication ◽  
K562 Cells ◽  
Cell Cytotoxicity ◽  
Nk Cell Cytotoxicity

Background: In peripheral blood, human natural killer (NK) cells are immunological cells that nearly don’t express the ectonucleotidase CD73 on their plasma membrane. When exposed to mesenchymal stem cells (MSCs), NK cells are able to acquire CD73. MSCs are known to be CD73-positive (CD73+) and also to modulate the immune system, e.g. through adenosynergic pathway by ectonucleosidases, such as CD73. Extracellular vesicles (EVs) are involved in cell-to-cell communication. Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have emerged as paracrine mediators that are part of MSC immunomodulatory effects including immunosuppressive properties and immune privilege. Objective: The aim of our work was to study if CD73 could be acquired by NK cells through cell-to-cell communication with MSC-EVs as cell culture additives. We also hypothesised that MSC-EVs would act as tolerance inducers to attenuate NK cell cytotoxicity. Methods: Cell isolation was made from human umbilical cords for MSCs and from human peripheral blood for NK cells. MSC-EVs were isolated by ultracentrifugation and filtration, then characterized by nanoparticle tracking assay and flow cytometry (CD9, 63, 81 and 73). MSC-EV interaction with NK cells was monitored by PKH67 staining. NK cell activation was followed by measuring the expression of CD73 and NK-activating receptor natural-killer group 2, member D (NKG2D) by flow cytometry. The cytotoxicity of NK cells or EV-conditioned NK cells was evaluated after co-culture with K562 cells. Results: We showed that MSC-EVs are nanoparticles able to express CD73 and interact with NK cells. MSC-EV conditioned NK cells seem to increase CD73 and decrease NKG2D through an EV-mediated mechanism. MSC-EVs have an immunosuppressive effect on NK cells by preventing NK cell activation and NK cell cytotoxicity towards K562 cells. Conclusions: Our results demonstrate that MSC-EVs could influence NK cell behaviour and act as immunosuppressant cell-based products.

scholarly journals Expression Patterns and Function of Chromatin Protein HMGB2 during Mesenchymal Stem Cell Differentiation

2011 ◽  
Vol 286 (48) ◽  
pp. 41489-41498 ◽  
Author(s):  
Noboru Taniguchi ◽  
Beatriz Caramés ◽  
Emily Hsu ◽  
Stephanie Cherqui ◽  
Yasuhiko Kawakami ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Mesenchymal Stem Cell ◽  
Expression Patterns ◽  
Stem Cell Differentiation ◽  
Chromatin Protein ◽  
Mesenchymal Stem Cell Differentiation ◽  
And Function

scholarly journals Human bone marrow-derived mesenchymal stem cell gene expression patterns vary with culture conditions

2013 ◽  
Vol 48 (2) ◽  
pp. 107 ◽  
Author(s):  
Myoung Woo Lee ◽  
Dae Seong Kim ◽  
Keon Hee Yoo ◽  
Hye Ryung Kim ◽  
In Keun Jang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Expression Patterns ◽  
Culture Conditions ◽  
Gene Expression Patterns ◽  
Cell Gene Expression ◽  
Cell Gene ◽  
Stem Cell Gene

scholarly journals Mesenchymal stem cell‐glioblastoma interactions mediated via kinin receptors unveiled by cytometry

2021 ◽  
Vol 99 (2) ◽  
pp. 152-163
Author(s):  
Micheli Mainardi Pillat ◽  
Ágatha Oliveira‐Giacomelli ◽  
Mona Neves Oliveira ◽  
Roberta Andrejew ◽  
Natalia Turrini ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Kinin Receptors

scholarly journals Effects of active acromegaly on bone mRNA and microRNA expression patterns

2018 ◽  
Vol 178 (4) ◽  
pp. 353-364 ◽  
Author(s):  
Zhanna Belaya ◽  
Tatiana Grebennikova ◽  
Galina Melnichenko ◽  
Alexey Nikitin ◽  
Alexander Solodovnikov ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Tissue ◽  
Quantitative Polymerase Chain Reaction ◽  
Expression Patterns ◽  
Sella Turcica ◽  
Compensatory Mechanisms ◽  
Tissue Samples ◽  
Active Acromegaly ◽  
Cell Commitment

Objective To evaluate the response of bone to chronic long-term growth hormone (GH) and insulin-like growth factor-1 (IGF1) excess by measuring the expression of selected mRNA and microRNA (miR) in bone tissue samples of patients with active acromegaly. Design Case–control study. Methods Bone tissue samples were obtained during transsphenoidal adenomectomy from the sphenoid bone (sella turcica) from 14 patients with clinically and biochemically confirmed acromegaly and 10 patients with clinically non-functioning pituitary adenoma (NFPA) matched by sex and age. Expression of genes involved in the regulation of bone remodeling was studied using quantitative polymerase chain reaction (qPCR). Results Of the genes involved in osteoblast and osteoclast activity, only alkaline phosphatase (ALP) mRNA was 50% downregulated in patients with acromegaly. GH excess caused increased expression of the Wnt signaling antagonists (DKK1) and agonists (WNT10B) and changes in the levels of miR involved in mesenchymal stem cell commitment to chondrocytes (miR-199a-5p) or adipocytes (miR-27-5p, miR-125b-5p, miR-34a-5p, miR-188-3p) P < 0.05; q < 0.1. Relevant compensatory mechanisms were found through the changes in miR involved in osteoblastogenesis (miR-210-5p, miR-135a-5p, miR-211, miR-23a-3p, miR-204-5p), but the expression of TWIST1 was 50% downregulated and RUNX2 was unchanged. Conclusions Acromegaly had minimal effects on tested mRNAs specific to osteoblast or osteoclast function except for downregulated ALP expression. The expressions of miR known to be involved in mesenchymal stem cell commitment and downregulated TWIST1 expression suggest acromegaly has a negative effect on osteoblastogenesis.

scholarly journals Human placenta mesenchymal stem cell-derived exosomes delay H2O2-induced aging in mouse cholangioids

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Wenyi Chen ◽  
Jiaqi Zhu ◽  
Feiyan Lin ◽  
Yanping Xu ◽  
Bing Feng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Human Placenta ◽  
Group Treatment ◽  
Therapeutic Potential ◽  
Expression Patterns ◽  
Control Group ◽  
Biliary Cirrhosis ◽  
Protective Effects

Abstract Background Cholangiocyte senescence is an important pathological process in diseases such as primary sclerosing cholangitis (PSC) and primary biliary cirrhosis (PBC). Stem cell/induced pluripotent stem cell-derived exosomes have shown anti-senescence effects in various diseases. We applied novel organoid culture technology to establish and characterize cholangiocyte organoids (cholangioids) with oxidative stress-induced senescence and then investigated whether human placenta mesenchymal stem cell (hPMSC)-derived exosomes exerted a protective effect in senescent cholangioids. Methods We identified the growth characteristics of cholangioids by light microscopy and confocal microscopy. Exosomes were introduced concurrently with H2O2 into the cholangioids. Using immunohistochemistry and immunofluorescence staining analyses, we assessed the expression patterns of the senescence markers p16INK4a, p21WAF1/Cip1, and senescence-associated β-galactosidase (SA-β-gal) and then characterized the mRNA and protein expression levels of chemokines and senescence-associated secretory phenotype (SASP) components. Results Well-established cholangioids expressed cholangiocyte-specific markers. Oxidative stress-induced senescence enhanced the expression of the senescence-associated proteins p16INK4a, p21WAF1/Cip1, and SA-β-gal in senescent cholangioids compared with the control group. Treatment with hPMSC-derived exosomes delayed the cholangioid aging progress and reduced the levels of SASP components (i.e., interleukin-6 and chemokine CC ligand 2). Conclusions Senescent organoids are a potential novel model for better understanding senescence progression in cholangiocytes. hPMSC-derived exosomes exert protective effects against senescent cholangioids under oxidative stress-induced injury by delaying aging and reducing SASP components, which might have therapeutic potential for PSC or PBC.

scholarly journals Extracellular Vesicle-Shuttled mRNA in Mesenchymal Stem Cell Communication

Stem Cells ◽  
2017 ◽  
Vol 35 (4) ◽  
pp. 1093-1105 ◽  
Author(s):  
Enrico Ragni ◽  
Federica Banfi ◽  
Mario Barilani ◽  
Alessandro Cherubini ◽  
Valentina Parazzi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Communication ◽  
Extracellular Vesicle
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