scholarly journals Determination of sperm concentration using flow cytometry with simultaneous analysis of sperm plasma membrane integrity in zebrafishDanio rerio

2015 ◽  
Vol 89 (4) ◽  
pp. 350-356 ◽  
Author(s):  
Huiping Yang ◽  
Jonathan Daly ◽  
Terrence R. Tiersch
Keyword(s):  
Flow Cytometry ◽  
Plasma Membrane ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Simultaneous Analysis ◽  
Plasma Membrane Integrity ◽  
Sperm Plasma Membrane

scholarly journals The effect of carob (Ceratonia siliqua) bean extract on male New Zealand White rabbit semen

2018 ◽  
Vol 26 (3) ◽  
pp. 209 ◽  
Author(s):  
A. Ata ◽  
O. Yildiz-Gulay ◽  
S. Güngör ◽  
A. Balic ◽  
M.S. Gulay
Keyword(s):  
Plasma Membrane ◽  
New Zealand ◽  
Plasma Concentration ◽  
Seminal Plasma ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Ceratonia Siliqua ◽  
Plasma Membrane Integrity ◽  
Protein Levels ◽  
Sperm Plasma Membrane

<p>The carob tree (Ceratonia siliqua) grows naturally in the Mediterranean region. The empiric use of carob cures for their aphrodisiac properties is very common in Turkey. Thus, the experiment was conducted to determine the effects of carob bean extracts on some reproductive parameters in male New Zealand White rabbits. During the adaptation period (stage 1), 6-8 mo old rabbits were trained in semen collection for 30 d. At the beginning of the treatment period (stage 2), rabbits were assigned randomly to 2 groups of 8 animals each. For a period of 49 d (1 spermatogenesis duration), one group was treated with a daily oral dose (10 mL) of carob extract and the other group received the corresponding volume of tap water. Semen was collected weekly. Semen samples taken at week 1 and 7 were analysed separately. At the beginning of stage 2, no differences were observed in the volume and pH of the ejaculate, sperm concentration, percentage of motility, percentage of live spermatozoa, percentage of sperm plasma membrane integrity, plasma concentration of testosterone, and seminal plasma protein levels between the control and carob extract treated animals. Similarly, at the end of stage 2, there were no differences in the volume and pH of the ejaculate, motility percentage, the percentage of live spermatozoa, percentage of sperm plasma membrane integrity, and the seminal plasma protein levels between the control and the carob extract treated animals. However, sperm concentration (P&lt;0.05), plasma concentration of testosterone (P&lt;0.05), and percentage of change in spermatozoa concentration (P&lt;0.02) between groups were affected at the end of stage 2. The data suggested that the use of carob cures prepared by boiling carob fruit could have beneficial influences on sperm concentration in rabbits.</p>

scholarly journals Sheng Jing Decoction Can Promote Spermatogenesis and Increase Sperm Motility of the Oligozoospermia Mouse Model

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Guang Yan ◽  
Fang Tian ◽  
Peng Liu ◽  
Jianming Sun ◽  
Jianmin Mao ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Sperm Motility ◽  
Sperm Morphology ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Testicular Tissue ◽  
Whole Body ◽  
Plasma Membrane Integrity ◽  
Normal Sperm ◽  
Sperm Plasma Membrane

Sheng Jing Decoction (SJD), as a traditional Chinese medicine prescription, is mainly be used to treat male infertility. However, the pharmacological functions and molecular mechanisms of SJD are poorly understood. In this study, we investigated the functions of SJD on spermatogenesis and sperm motility and explored the potential mechanisms involved. Here, we demonstrated that high, medium, and low doses of SJD are effective in restoring the impairments of the whole body and testicular tissue by cyclophosphamide inducing and to rescue the damage of testicular tissue cells including Sertoli cells and germ cells. SJD can partly restore the decrease in sperm concentration, sperm vitality, sperm motility, and normal sperm morphology rate in oligozoospermic mouse models. Ki67 staining analyses confirm SJD can promote testicular tissue cell proliferation. Real-time RT-PCR analyses also reveal that SJD can upregulate the expression of proliferation-associated gene Lin28a and differentiation-associated genes Kit, Sohlh2, and Stra8. SJD can also reduce the impairment of mitochondrial membrane potential (MMP) and sperm plasma membrane integrity by cyclophosphamide inducing. Our results reveal that SJD is effective in improving both sperm quantity and quality by increasing the sperm concentration, sperm vitality, sperm motility, and normal sperm morphology rate. SJD can promote spermatogenesis by upregulating the expression of the proliferation-associated gene Lin28a and the differentiation-associated genes (Kit, Sohlh2, and Stra8). SJD can sustain MMP and sperm plasma membrane integrity to increase sperm motility.

scholarly journals Sheng Jing Decoction, as a Traditional Chinese Medicine Prescription, Can Promote Spermatogenesis and Increase Sperm Motility

2021 ◽  
Author(s):  
Guang Yan ◽  
Fang Tian ◽  
Peng Liu ◽  
Jianmin Mao ◽  
Jianming Sun ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Sperm Motility ◽  
Sperm Morphology ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Testicular Tissue ◽  
Plasma Membrane Integrity ◽  
Medicine Prescription ◽  
Sperm Plasma Membrane ◽  
Chinese Medicine Prescription

Abstract BackgroundSheng Jing Decoction (SJD), as a traditional Chinese medicine prescription, is mainly be used to treat male infertility. It has been applied in many hospitals in China, and the clinical effect of patients' reaction is satisfactory. However, pharmacological function and molecular mechanism of SJD are poorly understood. In this study, mainly investigated the function of SJD on spermatogenesis and sperm motility, and explored the potential mechanisms.MethodsThe oligozoospermia model of ICR mice was induced by injecting intraperitoneally (ip) with 60 mg/kg dose of cyclophosphamide. At the same time of modeling, high, medium and low doses of SJD were given orally for treatment respectively. Sperm vitality and motility were detected and analyzed by CASA, and sperm morphology was tested by Papanicolaou staining. Sperm mitochondrial membrane potential (MMP) was tested by JC-1 staining. Sperm plasma membrane integrity was determined by SYBR-14/PI double staining. Histopathological changes of testis were analyzed by HE staining and Immunohistochemistry. Genes expression related to spermatogenesis and sperm development were detected by real-time RT-PCR.Results High, medium and low doses of SJD are effective to recover from the impairment of the whole body and testicular tissue by cyclophosphamide inducing, and to rescue the damage of testicular tissue cells including sertoli cells and germ cells by cyclophosphamide inducing. SJD can all partly restore the decrease in sperm concentration, sperm vitality, sperm motility and normal sperm morphology rate by cyclophosphamide inducing. Ki67 staining analyses confirm SJD can promoted testicular tissue cells proliferation. Real-time RT-PCR analyses reveal SJD can up-regulates the expression of proliferation-associated gene Lin28a, and differentiation-associated genes Kit, Sohlh2 and Stra8. SJD can also reduce the impairment of MMP and sperm plasma membrane integrity by cyclophosphamide inducing.ConclusionSJD is effective to support sperm quantity and quality by increasing sperm concentration, sperm vitality, sperm motility and normal sperm morphology rate. SJD can promote spermatogenesis by up-regulating the expression of the proliferation-associated gene Lin28a, and the differentiation-associated genes (Kit, Sohlh2 and Stra8). SJD can sustain MMP and sperm plasma membrane integrity to increase sperm motility.

The effects of chronic administration of ghrelin on rat sperm quality and membrane integrity

2009 ◽  
Vol 59 (2) ◽  
pp. 159-168 ◽  
Author(s):  
Arash Kheradmand ◽  
Majid Taati ◽  
Homayoon Babaei
Keyword(s):  
Plasma Membrane ◽  
Treatment Group ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Chronic Administration ◽  
Control Group ◽  
Plasma Membrane Integrity ◽  
Significant Difference

AbstractAlthough ghrelin acts as a modulator of feeding behavior and energy metabolism in the central nervous system, recent studies have implicated the peripheral actions of ghrelin in reproductive tissues. Here, we investigated the effects of chronic administration of ghrelin on the motility, plasma membrane integrity and concentration of rat spermatozoa. 45-d male Wistar rats were scheduled for the study and were divided into control and treatment groups. In the treatment group, 1 nmol of ghrelin was administered as sc injection for 10 consecutive days or vehicle (physiological saline) to the control rats. Sperm collection was achieved by killing of the rats on days 15, 25 and 50 after first injection. Total sperm motility and forward progressive movement did not exhibit significant difference during the experiment, although, there was a tendency for greater motion rate on d 15 and 25 in the treated rats compared to the control group. Plasma membrane integrity (HOS-reacted spermatozoa) was significantly higher in the treated animals, especially on day 15 as well as day 25, because of possible antioxidant properties of ghrelin. This value was statistically higher on day 15 than that of day 25 (P <0.05). Likewise, there was a significant correlation between the FPM (P <0.0001, r = 0.79) and TSM (P <0.01, r = 0.52) with the HOS test percentage in the treatment group. It was not observed statistically difference in the sperm concentration between groups during all of the experimental days. In conclusion, chronic administration of ghrelin (similar to induced by energy deficiency such as fasting) increased the integrity of sperm membrane, however, the sperm motility and concentration did not display any alterations.

scholarly journals Comparison of electronic volume and forward scatter principles of cell selection using flow cytometry for the evaluation of acrosome and plasma membrane integrity of bull spermatozoa

2014 ◽  
Vol 85 (8) ◽  
pp. 719-728 ◽  
Author(s):  
Fride Berg Standerholen ◽  
Frøydis Deinboll Myromslien ◽  
Elisabeth Kommisrud ◽  
Erik Ropstad ◽  
Karin Elisabeth Waterhouse
Keyword(s):  
Flow Cytometry ◽  
Plasma Membrane ◽  
Membrane Integrity ◽  
Cell Selection ◽  
Forward Scatter ◽  
Plasma Membrane Integrity

Plasma membrane changes during the liquid storage of boar spermatozoa: A comparison of methods

2010 ◽  
Vol 58 (1) ◽  
pp. 105-116 ◽  
Author(s):  
Dariusz Gączarzewicz ◽  
Małgorzata Piasecka ◽  
Jan Udała ◽  
Barbara Błaszczyk ◽  
Tomasz Stankiewicz ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Sperm Motility ◽  
Semen Analysis ◽  
Membrane Integrity ◽  
Live Cells ◽  
Lower Percentage ◽  
Loss Of Function ◽  
Plasma Membrane Integrity ◽  
Sperm Plasma Membrane ◽  
Semen Preservation

Studies were performed on boar semen routinely used at the local artificial insemination (AI) centre. The semen was stored in a Safe Cell Plus commercial extender at 17 °C for nine days. The aim of our research was focused on changes in sperm plasma membrane integrity. The integrity of the sperm plasma membrane and acrosome as well as sperm motility decreased after dilution and during storage of the semen. The highest percentage of live sperm was identified by the eosin-nigrosin method, a lower percentage by the SYBR-14/PI test, and the lowest percentage of live cells was discovered by the hypoosmotic swelling (HOS) test (P < 0.01). There were significant differences between the results of staining methods and sperm motility (P < 0.01). No significant differences were found between the HOS test results and sperm motility. The plasma membrane integrity parameters positively correlated (P < 0.001) with each other and with sperm motility but negatively with aspartate aminotransferase activity. Our findings confirmed that the boar sperm aging changes, which increased during liquid semen preservation, were connected with the loss of function and integrity of the sperm plasma membrane. The employed complementary tests are comprehensive indicators of sperm membrane integrity during long-term semen preservation, and they can help establish the actual number of ‘healthy’ cells. The assays may be used in AI laboratories and should be incorporated into the routine of semen analysis.

scholarly journals Semen Quality of Garut Rams feed by Different Protein Sources and Their Implementation Potential in Small Farms of West Java

2020 ◽  
Vol 20 (1) ◽  
pp. 47-55
Author(s):  
Kurnia Bagus Ariyanto ◽  
Lilis Khotijah ◽  
Dewi Apri Astuti ◽  
Raden Iis Arifiantini ◽  
Jean-Baptise Menassol
Keyword(s):  
Plasma Membrane ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Black Soldier Fly ◽  
Hermetia Illucens ◽  
Brachiaria Humidicola ◽  
Plasma Membrane Integrity ◽  
Protein Sources ◽  
Sperm Plasma Membrane

ABSTRACT. Maggot Hermetia illucens (Maggot Black Soldier Fly, MBSF) is an alternative protein source besides soybean meal (SBM) which may be used as a feed for improving the quality of semen particularly in Garut rams to support prolific nature. The aims of this study were to analyzed and compare the impact of different protein sources in feed on semen quality of Garut rams, and to assess the prediction ability of Garut rams to serve ewe in small-scale breeders in West Java, Indonesia. This study was conducted using a completely randomized design with 3 treatments and 4 replications, consisted of Brachiaria humidicola (BH) grass and T1 (concentrate contains 20% of SBM), T2 (concentrate contains 10% of SBM and 10% of MBSF), and T3 (concentrate contains 20% of MBSF). The parameters measured were feed consumption, semen quality (macroscopic and microscopic characteristics), also a potential ability of rams to serve ewe. The results showed there were no significant effect on protein consumption, semen volume, semen pH, semen color and consistency, sperm mass movement, sperm motility, sperm concentration, sperm morphology, and prediction potential ability to serve ewe. However, the result showed a significant effect (P0.05) on sperm viability and sperm plasma membrane integrity. Sperm plasma membrane integrity of ram feed with T3 was better than T1 and T2 (P0.05). The prediction potential ability rams to serve ewes on MBSF treatment was 38 heads, while in T1 and T2 were 43 and 57 heads, respectively. In conclusion, MBSF can be an alternative source of protein besides SBM to improve the semen quality of Garut rams.  ABSTRAK. Maggot Hermetia illucens (Maggot Black Soldier Fly; MBSF) adalah sumber protein alternatif selain bungkil kedelai (SBM) yang dapat dipergunakan sebagai pakan untuk memperbaiki kualitas semen terutama pada domba Garut untuk mendukung sifat prolifik. Tujuan penelitian ini adalah menganalisis dan membandingkan dampak pemberian sumber protein berbeda terhadap kualitas semen domba Garut dan untuk menilai kemampuan domba Garut pejantan dalam melayani betina pada peternakan rakyat di Jawa Barat, Indonesia. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan 4 ulangan yang terdiri dari rumput Brachiaria humidicola (BH) dan T1 (konsentrat mengandung 20% SBM), T2 (konsentrat mengandung 10% SBM dan 10% MBSF), dan T3 (konsentrat mengandung 20% MBSF). Parameter yang diukur adalah konsumsi pakan, karakteristik semen (makroskopis dan mikroskopis) serta potensi domba jantan melayani betina. Hasil Penelitian menunjukkan tidak ada perbedaan signifikan pada konsumsi protein pakan, volume semen, pH semen, warna dan konsistensi semen, gerakan massa sperma, motilitas sperma, konsentrasi sperma, morfologi sperma, dan prediksi potensi pejantan dalam melayani betina. Namun, hasil penelitian menunjukkan terdapat perbedaan (P0.05) pada viabilitas sperma dan membran plasma utuh sperma. Membran plasma utuh pada perlakuan T3 lebih baik dibandingkan perlakuan T1 dan T2 (P0.05). Prediksi potensi betina terlayani dari pejantan yang diberi pakan MBSF adalah 38 ekor, sedangkan yang diberi SBM dan kombinasinya adalah 43 dan 57 ekor. Kesimpulan penelitian ini adalah MBSF dapat menjadi alternatif sumber protein selain bungkil kedelai dalam memperbaiki kualitas sperma domba Garut.

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