Near infrared in vivo flow cytometry for tracking fluorescent circulating cells

2015 ◽  
Vol 87 (9) ◽  
pp. 878-884 ◽  
Author(s):  
Yuanzhen Suo ◽  
Tao Liu ◽  
Chengying Xie ◽  
Dan Wei ◽  
Xu Tan ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Near Infrared ◽  
Circulating Cells ◽  
In Vivo Flow Cytometry

scholarly journals In Vivo Flow Cytometry of Extremely Rare Circulating Cells

2018 ◽  
Author(s):  
Xuefei Tan ◽  
Roshani Patil ◽  
Peter Bartosik ◽  
Judith Runnels ◽  
Charles P. Lin ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cancer Metastasis ◽  
Near Infrared ◽  
Small Animal ◽  
Skin Surface ◽  
Signal Processing Algorithm ◽  
Circulating Blood Volume ◽  
Circulating Cells ◽  
In Vivo Flow Cytometry

Circulating tumor cells (CTCs) are of great interest in cancer research, but methods for their enumeration remain far from optimal. We developed a new small animal research tool called Diffuse in vivo Flow Cytometry (DiFC) for detecting extremely rare fluorescently- labeled circulating cells directly in the bloodstream. The technique exploits near-infrared diffuse photons to detect and count cells flowing in large superficial arteries and veins without drawing blood samples. DiFC uses custom-designed, dual fiber optic probes that are placed in contact with the skin surface approximately above a major vascular bundle. In combination with a novel signal processing, algorithm DiFC allows counting of individual cells moving in arterial or venous directions, as well as measurement of their speed and depth. We show that DiFC allows sampling of the entire circulating blood volume of a mouse in under 10 minutes, while maintaining a false alarm rate of 0.014 per minute. Hence, the unique capabilities of DiFC are highly suited to biological applications involving very rare cell types such as the study of hematogenic cancer metastasis.

Computer Vision In Vivo Flow Cytometry of Low-Abundance Circulating Cells

2015 ◽  
Author(s):  
Stacey Markovic ◽  
Siyuan Li ◽  
Octavia Camps ◽  
Mario Sznaier ◽  
Mark Niedre
Keyword(s):  
Computer Vision ◽  
Flow Cytometry ◽  
Circulating Cells ◽  
In Vivo Flow Cytometry

scholarly journals Recent advances in fluorescence-basedin vivoflow cytometry

2019 ◽  
Vol 12 (06) ◽  
pp. 1930008
Author(s):  
Kai Pang ◽  
Bobo Gu ◽  
Feng Liu ◽  
Mingli Dong ◽  
Lianqing Zhu ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Detection Methods ◽  
Biological Applications ◽  
Label Free ◽  
Circulating Cells ◽  
Recent Advances ◽  
Label Free Detection ◽  
In Vivo Flow Cytometry ◽  
Biological Environment

The fluorescence-based in vivo flow cytometry (IVFC) is an emerging tool to monitor circulating cells in vivo. As a noninvasive and real-time diagnostic technology, the fluorescence-based IVFC allows long-term monitoring of circulating cells without changing their native biological environment. It has been applied for various biological applications (e.g., monitoring circulating tumor cells). In this work, we will review our recent works on fluorescence-based IVFC. The operation principle and typical biological applications will be introduced. In addition, the recent advances in IVFC flow cytometry based on photoacoustic effects and other label-free detection methods such as imaging-based methods, diffuse-light methods, hybrid multimodality methods and multispectral methods are also summarized.

scholarly journals In Vivo Flow Cytometry of Extremely Rare Circulating Cells

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Xuefei Tan ◽  
Roshani Patil ◽  
Peter Bartosik ◽  
Judith M. Runnels ◽  
Charles P. Lin ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Circulating Cells ◽  
In Vivo Flow Cytometry

scholarly journals Cell counting for in vivo flow cytometry signals with baseline drift

2017 ◽  
Vol 10 (03) ◽  
pp. 1750008 ◽  
Author(s):  
Xiaoling Wang ◽  
Yuanzhen Suo ◽  
Dan Wei ◽  
Hao He ◽  
Fan Wu ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Mean Squared Error ◽  
Automatic Segmentation ◽  
Cell Counting ◽  
Target Cells ◽  
Baseline Drift ◽  
Research Fields ◽  
Circulating Cells ◽  
In Vivo Flow Cytometry

In biomedical research fields, the in vivo flow cytometry (IVFC) is a widely used technology which is able to monitor target cells dynamically in living animals. Although the setup of IVFC system has been well established, baseline drift is still a challenge in the process of quantifying circulating cells. Previous methods, i.e., the dynamic peak picking method, counted cells by setting a static threshold without considering the baseline drift, leading to an inaccurate cell quantification. Here, we developed a method of cell counting for IVFC data with baseline drift by interpolation fitting, automatic segmentation and wavelet-based denoising. We demonstrated its performance for IVFC signals with three types of representative baseline drift. Compared with non-baseline-correction methods, this method showed a higher sensitivity and specificity, as well as a better result in the Pearson’s correlation coefficient and the mean-squared error (MSE).

scholarly journals Imaging Cardiovascular and Lung Macrophages With the Positron Emission Tomography Sensor 64 Cu-Macrin in Mice, Rabbits, and Pigs

2020 ◽  
Vol 13 (10) ◽  
Author(s):  
Matthias Nahrendorf ◽  
Friedrich Felix Hoyer ◽  
Anu E. Meerwaldt ◽  
Mandy M.T. van Leent ◽  
Max L. Senders ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Flow Cytometry ◽  
Confocal Microscopy ◽  
Pet Imaging ◽  
Near Infrared ◽  
Emission Tomography ◽  
Imaging Biomarker ◽  
Positron Emission ◽  
Pulmonary Macrophages

Background: Macrophages, innate immune cells that reside in all organs, defend the host against infection and injury. In the heart and vasculature, inflammatory macrophages also enhance tissue damage and propel cardiovascular diseases. Methods: We here use in vivo positron emission tomography (PET) imaging, flow cytometry, and confocal microscopy to evaluate quantitative noninvasive assessment of cardiac, arterial, and pulmonary macrophages using the nanotracer 64 Cu-Macrin—a 20-nm spherical dextran nanoparticle assembled from nontoxic polyglucose. Results: PET imaging using 64 Cu-Macrin faithfully reported accumulation of macrophages in the heart and lung of mice with myocardial infarction, sepsis, or pneumonia. Flow cytometry and confocal microscopy detected the near-infrared fluorescent version of the nanoparticle ( VT680 Macrin) primarily in tissue macrophages. In 5-day-old mice, 64 Cu-Macrin PET imaging quantified physiologically more numerous cardiac macrophages. Upon intravenous administration of 64 Cu-Macrin in rabbits and pigs, we detected heightened macrophage numbers in the infarcted myocardium, inflamed lung regions, and atherosclerotic plaques using a clinical PET/magnetic resonance imaging scanner. Toxicity studies in rats and human dosimetry estimates suggest that 64 Cu-Macrin is safe for use in humans. Conclusions: Taken together, these results indicate 64 Cu-Macrin could serve as a facile PET nanotracer to survey spatiotemporal macrophage dynamics during various physiological and pathological conditions. 64 Cu-Macrin PET imaging could stage inflammatory cardiovascular disease activity, assist disease management, and serve as an imaging biomarker for emerging macrophage-targeted therapeutics.

Monitoring circulating apoptotic cells by in-vivo flow cytometry

2008 ◽  
Author(s):  
Xunbin Wei ◽  
Yuan Tan ◽  
Yun Chen ◽  
Li Zhang ◽  
Yan Li ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Apoptotic Cells ◽  
In Vivo Flow Cytometry

scholarly journals Cell labeling approaches for fluorescence-based in vivo flow cytometry

2011 ◽  
Vol 79A (10) ◽  
pp. 758-765 ◽  
Author(s):  
Costas M. Pitsillides ◽  
Judith M. Runnels ◽  
Joel A. Spencer ◽  
Liang Zhi ◽  
Mei X. Wu ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Labeling ◽  
In Vivo Flow Cytometry
Sign in / Sign up

Export Citation Format

Share Document