NCAM polysialylation during adherence transitions: Live cell monitoring using an antibody-mimetic EGFP-endosialidase and the viability dye DRAQ7

2013 ◽  
Vol 83A (7) ◽  
pp. 659-671 ◽  
Author(s):  
Paul J. Smith ◽  
Emeline Furon ◽  
Marie Wiltshire ◽  
Sally Chappell ◽  
Laurence H. Patterson ◽  
...  
Keyword(s):  
Live Cell ◽  
Cell Monitoring

scholarly journals Live Cell Monitoring of μ-Opioid Receptor-mediated G-protein Activation Reveals Strong Biological Activity of Close Morphine Biosynthetic Precursors

2007 ◽  
Vol 282 (37) ◽  
pp. 27126-27132 ◽  
Author(s):  
Viacheslav O. Nikolaev ◽  
Chotima Boettcher ◽  
Christian Dees ◽  
Moritz Bünemann ◽  
Martin J. Lohse ◽  
...  
Keyword(s):  
Biological Activity ◽  
G Protein ◽  
Opioid Receptor ◽  
Live Cell ◽  
Protein Activation ◽  
G Protein Activation ◽  
Cell Monitoring ◽  
Μ Opioid Receptor

scholarly journals Live Cell Monitoring of hiPSC Generation and Differentiation Using Differential Expression of Endogenous microRNAs

PLoS ONE ◽  
2010 ◽  
Vol 5 (7) ◽  
pp. e11834 ◽  
Author(s):  
Masakazu Kamata ◽  
Min Liang ◽  
Shirley Liu ◽  
Yoshiko Nagaoka ◽  
Irvin S. Y. Chen
Keyword(s):  
Differential Expression ◽  
Live Cell ◽  
Cell Monitoring

Design and Characterization of a Fluorescent Reporter Enabling Live-cell Monitoring of MCT8 Expression

2021 ◽  
Author(s):  
Adina Sophie Graffunder ◽  
Sarah Paisdzior ◽  
Robert Opitz ◽  
Kostja Renko ◽  
Peter Kühnen ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Functional Characterization ◽  
Live Cell ◽  
Monocarboxylate Transporter ◽  
Start Codon ◽  
Future Application ◽  
Gene Assay ◽  
Cell Monitoring

AbstractThe monocarboxylate transporter 8 (MCT8) is a specific thyroid hormone transporter and plays an essential role in fetal development. Inactivating mutations in the MCT8 encoding gene SLC16A2 (solute carrier family 16, member 2) lead to the Allan-Herndon-Dudley syndrome, a condition presenting with severe endocrinological and neurological phenotypes. However, the cellular distribution pattern and dynamic expression profile are still not well known for early human neural development. Objective Development and characterization of fluorescent MCT8 reporters that would permit live-cell monitoring of MCT8 protein expression in vitro in human induced pluripotent stem cell (hiPSC)-derived cell culture models. Methods A tetracysteine (TC) motif was introduced into the human MCT8 sequence at four different positions as binding sites for fluorescent biarsenical dyes. Human Embryonic Kidney 293 cells were transfected and stained with fluorescein-arsenical hairpin-binder (FlAsH). Counterstaining with specific MCT8 antibody was performed. Triiodothyronine (T3) uptake was indirectly measured with a T3 responsive luciferase-based reporter gene assay in Madin-Darby Canine Kidney 1 cells for functional characterization. Results FlAsH staining and antibody counterstaining of all four constructs showed cell membrane expression of all MCT8 constructs. The construct with the tag after the first start codon demonstrated comparable T3 uptake to the MCT8 wildtype. Conclusion Our data indicate that introduction of a TC-tag directly after the first start codon generates a MCT8 reporter with suitable characteristics for live-cell monitoring of MCT8 expression. One promising future application will be generation of stable hiPSC MCT8 reporter lines to characterize MCT8 expression patterns during in vitro neuronal development.

Live-Cell Probe for In Situ Single-Cell Monitoring of Mitochondrial DNA Methylation

ACS Sensors ◽  
2021 ◽  
Author(s):  
Han Zhao ◽  
Donghan Ma ◽  
Junkai Xie ◽  
Oscar Sanchez ◽  
Fang Huang ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Mitochondrial Dna ◽  
Single Cell ◽  
Live Cell ◽  
Cell Monitoring

scholarly journals LED-Based Tomographic Imaging for Live-Cell Monitoring of Pancreatic Islets in Microfluidic Channels

Proceedings ◽  
2017 ◽  
Vol 1 (4) ◽  
pp. 552 ◽  
Author(s):  
Gregor Scholz ◽  
Qifeng Xu ◽  
Torben Schulze ◽  
Heidi Boht ◽  
Kai Mattern ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Tomographic Imaging ◽  
Live Cell ◽  
Microfluidic Channels ◽  
Cell Monitoring

scholarly journals Conducting Polymer Scaffolds Based on Poly(3,4-ethylenedioxythiophene) and Xanthan Gum for Live-Cell Monitoring

ACS Omega ◽  
2018 ◽  
Vol 3 (7) ◽  
pp. 7424-7431 ◽  
Author(s):  
Isabel del Agua ◽  
Sara Marina ◽  
Charalampos Pitsalidis ◽  
Daniele Mantione ◽  
Magali Ferro ◽  
...  
Keyword(s):  
Conducting Polymer ◽  
Xanthan Gum ◽  
Live Cell ◽  
Polymer Scaffolds ◽  
Cell Monitoring

scholarly journals Antibody-Directed Lentiviral Gene Transduction for Live-Cell Monitoring and Selection of Human iPS and hES Cells

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e34778 ◽  
Author(s):  
Dai-tze Wu ◽  
Yasunari Seita ◽  
Xia Zhang ◽  
Chi-Wei Lu ◽  
Monica J. Roth
Keyword(s):  
Live Cell ◽  
Gene Transduction ◽  
Cell Monitoring ◽  
Hes Cells ◽  
Selection Of

scholarly journals DNA hydrogel microspheres and their potential applications for protein delivery and live cell monitoring

2016 ◽  
Vol 10 (3) ◽  
pp. 034112 ◽  
Author(s):  
Taeyoung Kim ◽  
Seongmin Park ◽  
Minhyuk Lee ◽  
Solhee Baek ◽  
Jong Bum Lee ◽  
...  
Keyword(s):  
Protein Delivery ◽  
Live Cell ◽  
Potential Applications ◽  
Cell Monitoring ◽  
Dna Hydrogel ◽  
Hydrogel Microspheres
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