Optimization of calyculin A-induced premature chromosome condensation assay for chromosome aberration studies

2011 ◽  
Vol 79A (12) ◽  
pp. 1016-1022 ◽  
Author(s):  
Tomisato Miura ◽  
William F. Blakely
Keyword(s):  
Chromosome Aberration ◽  
Chromosome Condensation ◽  
Calyculin A ◽  
Premature Chromosome Condensation

Transient inhibition of Calyculin A induced premature chromosome condensation by hyperthermia

2009 ◽  
Vol 25 (3) ◽  
pp. 220-228
Author(s):  
J. W. J. Bergs ◽  
J. W. J. Bergs ◽  
R. Ten Cate ◽  
H. M. Rodermond ◽  
P. A. Jaarsma ◽  
...  
Keyword(s):  
Chromosome Condensation ◽  
Calyculin A ◽  
Premature Chromosome Condensation

scholarly journals The Usefulness of Calyculin A for Cytogenetic Prenatal Diagnosis

2005 ◽  
Vol 53 (3) ◽  
pp. 391-394 ◽  
Author(s):  
Malgorzata I. Srebniak ◽  
Gizela G. Trapp ◽  
Angelika K. Wawrzkiewicz ◽  
Wojciech Kaźmierczak ◽  
Andrzej K. Wiczkowski
Keyword(s):  
Cell Cycle ◽  
Prenatal Diagnosis ◽  
Amniotic Fluid ◽  
Protein Phosphatases ◽  
Genetic Material ◽  
Chromosome Condensation ◽  
Calyculin A ◽  
Premature Chromosome Condensation ◽  
Chromosomal Breaks

An increased number of chromosome plates can be obtained by use of calyculin A (CLA). CLA is an inhibitor of protein phosphatases (type 1 and type 2A serine/threonine). Inactivation of these phosphatases leads to premature chromosome condensation (PCC) in all phases of the cell cycle; thus, it is possible to investigate both metaphase and G2-PCC chromosomes. Amniotic fluid (AF) cultures were treated with calyculin A (CLA). GTG banding was obtained. Using this method it is possible to investigate all cell cycle phases, GTG banding, chromosomal breaks, and rates of PCD on the same preparation. Analyses of AF cultures treated with CLA allow complex studies on fetal genetic material. This work presents potential usefulness of CLA for cytogenetic prenatal diagnosis.

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