Miniaturizing bromodeoxyuridine incorporation enables the usage of flow cytometry for cell cycle analysis of adherent tissue culture cells for high throughput screening

2010 ◽  
Vol 77A (10) ◽  
pp. 953-961 ◽  
Author(s):  
Paolo Cappella ◽  
Maria Laura Giorgini ◽  
Claudia Ernestina Re ◽  
Paolo Ubezio ◽  
Marina Ciomei ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Tissue Culture ◽  
High Throughput ◽  
High Throughput Screening ◽  
Cell Cycle Analysis ◽  
Culture Cells ◽  
Tissue Culture Cells

scholarly journals In VitroEfficacy of Antibiotics Commonly Used To Treat Human Plague against IntracellularYersinia pestis

2011 ◽  
Vol 55 (8) ◽  
pp. 3752-3757 ◽  
Author(s):  
Jered M. Wendte ◽  
Duraisamy Ponnusamy ◽  
Deanna Reiber ◽  
Jeffrey L. Blair ◽  
Kenneth D. Clinkenbeard
Keyword(s):  
Tissue Culture ◽  
High Throughput ◽  
Prophylactic Treatment ◽  
Intracellular Parasite ◽  
Broth Microdilution ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Initial Stage ◽  
Selection Of ◽  
Serial Dilutions

ABSTRACTYersinia pestisinitiates infection as a facultative intracellular parasite in host macrophages; however, little is known about the efficacy of antibiotics commonly used to treat human plague against intracellularY. pestis. Intracellular minimal bactericidal concentrations (MBCs) were determined using a high-throughput broth microdilution assay in which human THP-1 macrophage-like cells were infected withY. pestisstrain KIM6-2053.1+ and exposed to 2-fold serial dilutions of antibiotics for 24 h in 96-well plates. The numbers of CFU, upon which minimal bactericidal concentrations were based, were determined by counting “microcolonies” in wells of 96-well plates following lysis of tissue culture cells to release survivingY. pestis, replica dilution, and plating in soft tryptic soy broth agar. For THP-1 cells, streptomycin and ciprofloxacin had comparable efficacies for intra- and extracellularY. pestis, but the MBCs for chloramphenicol, gentamicin, doxycycline, and amoxicillin were two-, three-, four-, and five 2-fold serial dilutions greater, respectively, for intracellular than for extracellularY. pestis. During the initial stage of plague, intracellularY. pestismay be less susceptible to antibiotic killing by particular antibiotics recommended for treatment of plague, such as gentamicin or doxycycline, whereas others, such as streptomycin and ciprofloxacin, may have similar efficacies against extracellular or intracellularY. pestis. This may be of particular importance in the selection of antibiotics for prophylactic treatment in the case of a bioterrorism event.

Bioluminescent high-throughput assay for the bacteria adherence to the tissue culture cells

2011 ◽  
Vol 108 (7) ◽  
pp. 1628-1633 ◽  
Author(s):  
L. Brovko ◽  
O. Minikh ◽  
A. Piekna ◽  
M.W. Griffiths
Keyword(s):  
Tissue Culture ◽  
High Throughput ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
High Throughput Assay

scholarly journals High-Throughput RNA Interference Screens in Drosophila Tissue Culture Cells

2005 ◽  
pp. 55-73 ◽  
Author(s):  
Susan Armknecht ◽  
Michael Boutros ◽  
Amy Kiger ◽  
Kent Nybakken ◽  
Bernard Mathey-Prevot ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Rna Interference ◽  
High Throughput ◽  
Culture Cells ◽  
Tissue Culture Cells

scholarly journals Label-free cell cycle analysis for high-throughput imaging flow cytometry

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Thomas Blasi ◽  
Holger Hennig ◽  
Huw D. Summers ◽  
Fabian J. Theis ◽  
Joana Cerveira ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
High Throughput ◽  
Free Cell ◽  
Cell Cycle Analysis ◽  
Label Free ◽  
Imaging Flow Cytometry ◽  
High Throughput Imaging

Cytoplasmic Tubules in Cells Infected with Laryngotracheitis Virus

1969 ◽  
Vol 27 ◽  
pp. 376-377
Author(s):  
A. M. Watrach
Keyword(s):  
Tissue Culture ◽  
Small Proportion ◽  
Chicken Embryo ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Morphologic Characteristics ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus ◽  
In Cells

During a study of the development of infectious laryngotracheitis (LT) virus in tissue culture cells, unusual tubular formations were found in the cytoplasm of a small proportion of the affected cells. It is the purpose of this report to describe the morphologic characteristics of the tubules and to discuss their possible association with the development of virus.The source and maintenance of the strain of LT virus have been described. Prior to this study, the virus was passed several times in chicken embryo kidney (CEK) tissue culture cells.

Sulfhydryl bond formation is a prerequisite for proper cycling of centrosomes and chromosomes in mammalian tissue culture cells

1993 ◽  
Vol 51 ◽  
pp. 342-343
Author(s):  
Heide Schatten ◽  
Neidhard Paweletz ◽  
Ron Balczon
Keyword(s):  
Tissue Culture ◽  
Cell Cycle Progression ◽  
Group Formation ◽  
Sulfhydryl Group ◽  
Mammalian Tissue ◽  
Mitotic Chromosomes ◽  
Microtubule Network ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Transmission Electron

To study the role of sulfhydryl group formation during cell cycle progression, mammalian tissue culture cells (PTK2) were exposed to 100¼M 2-mercaptoethanol for 2 to 6 h during their exponential phase of growth. The effects of 2-mercaptoethanol on centrosomes, chromosomes, microtubules, membranes and intermediate filaments were analyzed by transmission electron microscopy (TEM) and by immunofluorescence microscopy (IFM) methods using a human autoimmune antibody directed against centrosomes (SPJ), and a mouse monoclonal antibody directed against tubulin (E7). Chromosomes were affected most by this treatment: premature chromosome condensation was detected in interphase nuclei, and the structure in mitotic chromosomes was altered compared to control cells. This would support previous findings in dividing sea urchin cells in which chromosomes are arrested at metaphase while the centrosome splitting cycle continues. It might also support findings that certairt-sulfhydryl-blocking agents block cyclin destruction. The organization of the microtubule network was scattered probably due to a looser organization of centrosomal material at the interphase centers and at the mitotic poles.

Sign in / Sign up

Export Citation Format

Share Document