scholarly journals In vivo multispectral, multiparameter, photoacoustic lymph flow cytometry with natural cell focusing, label-free detection and multicolor nanoparticle probes

2008 ◽  
Vol 73A (10) ◽  
pp. 884-894 ◽  
Author(s):  
Ekaterina I. Galanzha ◽  
Evgeny V. Shashkov ◽  
Valery V. Tuchin ◽  
Vladimir P. Zharov
Keyword(s):  
Flow Cytometry ◽  
Lymph Flow ◽  
Label Free ◽  
Nanoparticle Probes ◽  
Label Free Detection ◽  
Natural Cell

Noninvasive and label-free detection of circulating melanoma cells by in vivo photoacoustic flow cytometry

2015 ◽  
Author(s):  
Ping Yang ◽  
Rongrong Liu ◽  
Zhenyu Niu ◽  
Yuanzhen Suo ◽  
Hao He ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Melanoma Cells ◽  
Label Free ◽  
Label Free Detection

Label-free detection of circulating melanoma cells by in vivo photoacoustic flow cytometry

2016 ◽  
Author(s):  
Xiaoling Wang ◽  
Ping Yang ◽  
Rongrong Liu ◽  
Zhenyu Niu ◽  
Yuanzhen Suo ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Melanoma Cells ◽  
Label Free ◽  
Label Free Detection

scholarly journals Recent advances in fluorescence-basedin vivoflow cytometry

2019 ◽  
Vol 12 (06) ◽  
pp. 1930008
Author(s):  
Kai Pang ◽  
Bobo Gu ◽  
Feng Liu ◽  
Mingli Dong ◽  
Lianqing Zhu ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Detection Methods ◽  
Biological Applications ◽  
Label Free ◽  
Circulating Cells ◽  
Recent Advances ◽  
Label Free Detection ◽  
In Vivo Flow Cytometry ◽  
Biological Environment

The fluorescence-based in vivo flow cytometry (IVFC) is an emerging tool to monitor circulating cells in vivo. As a noninvasive and real-time diagnostic technology, the fluorescence-based IVFC allows long-term monitoring of circulating cells without changing their native biological environment. It has been applied for various biological applications (e.g., monitoring circulating tumor cells). In this work, we will review our recent works on fluorescence-based IVFC. The operation principle and typical biological applications will be introduced. In addition, the recent advances in IVFC flow cytometry based on photoacoustic effects and other label-free detection methods such as imaging-based methods, diffuse-light methods, hybrid multimodality methods and multispectral methods are also summarized.

scholarly journals In vivo, Noninvasive, Label-Free Detection and Eradication of Circulating Metastatic Melanoma Cells Using Two-Color Photoacoustic Flow Cytometry with a Diode Laser

2009 ◽  
Vol 69 (20) ◽  
pp. 7926-7934 ◽  
Author(s):  
Ekaterina I. Galanzha ◽  
Evgeny V. Shashkov ◽  
Paul M. Spring ◽  
James Y. Suen ◽  
Vladimir P. Zharov
Keyword(s):  
Flow Cytometry ◽  
Metastatic Melanoma ◽  
Diode Laser ◽  
Melanoma Cells ◽  
Label Free ◽  
Label Free Detection

Deep Learning-enabled Holographic Imaging Flow-Cytometry for Label-Free Detection of Giardia Lamblia in Water Samples

2021 ◽  
Author(s):  
Zoltán Göröcs ◽  
David Baum ◽  
Fang Song ◽  
Kevin de Haan ◽  
Hatice Ceylan Koydemir ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Deep Learning ◽  
Water Samples ◽  
Giardia Lamblia ◽  
Label Free ◽  
Holographic Imaging ◽  
Imaging Flow Cytometry ◽  
Label Free Detection

scholarly journals Dantrolene Prevents the Lymphostasis Caused by Doxorubicin in the Rat Mesenteric Circulation

2021 ◽  
Vol 12 ◽  
Author(s):  
Serena Van ◽  
Soumiya Pal ◽  
Brittney R. Garner ◽  
Kate Steed ◽  
Vijayalakshmi Sridharan ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Speed ◽  
Ryanodine Receptors ◽  
Lymph Flow ◽  
Free Calcium ◽  
Physiological Salt Solution ◽  
Breast Cancer Patients ◽  
Rapid Infusion ◽  
Prophylactic Use

Background and Purpose: Doxorubicin (DOX) is a risk factor for arm lymphedema in breast cancer patients. We reported that DOX opens ryanodine receptors (RYRs) to enact “calcium leak,” which disrupts the rhythmic contractions of lymph vessels (LVs) to attenuate lymph flow. Here, we evaluated whether dantrolene, a clinically available RYR1 subtype antagonist, prevents the detrimental effects of DOX on lymphatic function.Experimental Approach: Isolated rat mesenteric LVs were cannulated, pressurized (4–5 mm Hg) and equilibrated in physiological salt solution and Fura-2AM. Video microscopy recorded changes in diameter and Fura-2AM fluorescence tracked cytosolic free calcium ([Ca2+i]). High-speed in vivo microscopy assessed mesenteric lymph flow in anesthetized rats. Flow cytometry evaluated RYR1 expression in freshly isolated mesenteric lymphatic muscle cells (LMCs).Key Results: DOX (10 μmol/L) increased resting [Ca2+i] by 17.5 ± 3.7% in isolated LVs (n = 11). The rise in [Ca2+i] was prevented by dantrolene (3 μmol/L; n = 10). A single rapid infusion of DOX (10 mg/kg i.v.) reduced positive volumetric lymph flow to 29.7 ± 10.8% (n = 7) of baseline in mesenteric LVs in vivo. In contrast, flow in LVs superfused with dantrolene (10 μmol/L) only decreased to 76.3 ± 14.0% (n = 7) of baseline in response to DOX infusion. Subsequently, expression of the RYR1 subtype protein as the presumed dantrolene binding site was confirm in isolated mesenteric LMCs by flow cytometry.Conclusion and Implications: We conclude that dantrolene attenuates the acute impairment of lymph flow by DOX and suggest that its prophylactic use in patients subjected to DOX chemotherapy may lower lymphedema risk.

scholarly journals In vivo label-free measurement of lymph flow velocity and volumetric flow rates using Doppler optical coherence tomography

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Cedric Blatter ◽  
Eelco F. J. Meijer ◽  
Ahhyun S. Nam ◽  
Dennis Jones ◽  
Brett E. Bouma ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Flow Velocity ◽  
Lymph Flow ◽  
Optical Coherence ◽  
Label Free ◽  
Flow Rates ◽  
Doppler Optical Coherence Tomography
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