scholarly journals Quantification of green fluorescent protein by in vivo imaging, PCR, and flow cytometry: Comparison of transgenic strains and relevance for fetal cell microchimerism

2008 ◽  
Vol 73A (2) ◽  
pp. 11-118 ◽  
Author(s):  
Yutaka Fujiki ◽  
Kai Tao ◽  
Diana W. Bianchi ◽  
Maryann Giel-Moloney ◽  
Andrew B. Leiter ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Green Fluorescent Protein ◽  
In Vivo Imaging ◽  
Fluorescent Protein ◽  
Fetal Cell ◽  
Green Fluorescent ◽  
Transgenic Strains

Intraocular in vivo imaging of activated T-lymphocytes expressing green-fluorescent protein after stimulation with endotoxin

2001 ◽  
Vol 239 (8) ◽  
pp. 609-612 ◽  
Author(s):  
Matthias D. Becker ◽  
Sergio Crespo ◽  
Tammy M. Martin ◽  
Stephen R. Planck ◽  
Mayumi Naramura ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
T Lymphocytes ◽  
In Vivo Imaging ◽  
Fluorescent Protein ◽  
Activated T Lymphocytes ◽  
Green Fluorescent

scholarly journals Three-Dimensional in Vivo Imaging of Green Fluorescent Protein-Expressing T Cells in Mice with Noncontact Fluorescence Molecular Tomography

2007 ◽  
Vol 6 (2) ◽  
pp. 7290.2007.00007 ◽  
Author(s):  
Anikitos Garofalakis ◽  
Giannis Zacharakis ◽  
Heiko Meyer ◽  
Eleftherios N. Economou ◽  
Clio Mamalaki ◽  
...  
Keyword(s):  
T Cells ◽  
Green Fluorescent Protein ◽  
In Vivo Imaging ◽  
Fluorescent Protein ◽  
Three Dimensional ◽  
Fluorescence Molecular Tomography ◽  
Green Fluorescent

scholarly journals In Vivo Tracking of Campylobacter jejuni by Using a Novel Recombinant Expressing Green Fluorescent Protein

2003 ◽  
Vol 69 (5) ◽  
pp. 2864-2874 ◽  
Author(s):  
Philip F. Mixter ◽  
John D. Klena ◽  
Gary A. Flom ◽  
Amy M. Siegesmund ◽  
Michael E. Konkel
Keyword(s):  
Flow Cytometry ◽  
Green Fluorescent Protein ◽  
Campylobacter Jejuni ◽  
Fluorescent Protein ◽  
Developed Countries ◽  
Wild Type ◽  
Reporter System ◽  
Type Isolate ◽  
Green Fluorescent

ABSTRACT Campylobacter jejuni is a leading cause of food-borne disease in developed countries. The goal of this study was to develop a plasmid-based reporter system with green fluorescent protein (GFP) to facilitate the study of C. jejuni in a variety of niches. C. jejuni transformants harboring the pMEK91 GFP gene (gfp)-containing vector were readily detectable by both fluorescence microscopy and flow cytometry. Given the ease of detecting these organisms, additional experiments were performed in which BALB/c mice were injected intraperitoneally with C. jejuni harboring the gfp-containing vector. Four hours after injection of the mice, flow cytometry analyses determined that C. jejuni synthesizing GFP were predominantly associated with granulocytes. More specifically, the proportion of CD11b+ Gr-1+ lavage neutrophils with green fluorescence ranged from 99.7 to 100%, while the proportion of CD11b+ Gr-1− lavage macrophages ranged from 77.0 to 80.0%. In contrast, few CD11b− CD45R+ B lymphocytes from the lavage of the C. jejuni-injected mice were associated with green-fluorescent C. jejuni (proportions ranged from 0.75 to 0.77%). Cell-free C. jejuni was recovered from tissue homogenates after intraperitoneal injection. Macrorestriction profiling with pulsed-field gel electrophoresis identified a genotypic variant of the C. jejuni F38011 wild-type isolate. In vivo this variant displayed a phenotype identical to that of the wild-type isolate. In summary, we demonstrate that C. jejuni associates with marker-defined cellular subsets in vivo with a novel gfp reporter system and that C. jejuni genotypic variants can be isolated from both in vitro and in vivo systems.

scholarly journals A photoelectron imaging study of the deprotonated GFP chromophore anion and RNA fluorescent tags

2021 ◽  
Author(s):  
Joanne Woodhouse ◽  
Alice Henley ◽  
Ross Lewin ◽  
John M Ward ◽  
Helen Hailes ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
In Vivo Imaging ◽  
Fluorescent Protein ◽  
Imaging Study ◽  
Spectroscopic Studies ◽  
Photoelectron Imaging ◽  
Gfp Chromophore ◽  
Fluorescent Tags ◽  
Green Fluorescent

Green fluorescent protein (GFP), together with its family of variants, is the most widely used fluorescent protein for in vivo imaging. Numerous spectroscopic studies of the isolated GFP chromophore have...

scholarly journals Functional expression, quantification and cellular localization of the Hxt2 hexose transporter of Saccharomyces cerevisiae tagged with the green fluorescent protein

1999 ◽  
Vol 339 (2) ◽  
pp. 299-307 ◽  
Author(s):  
Arthur L. KRUCKEBERG ◽  
Ling YE ◽  
Jan A. BERDEN ◽  
Karel van DAM
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasma Membrane ◽  
Green Fluorescent Protein ◽  
Glucose Transport ◽  
Cellular Localization ◽  
Fluorescent Protein ◽  
Hexose Transporter ◽  
High Concentrations ◽  
Green Fluorescent

The Hxt2 glucose transport protein of Saccharomyces cerevisiae was genetically fused at its C-terminus with the green fluorescent protein (GFP). The Hxt2-GFP fusion protein is a functional hexose transporter: it restored growth on glucose to a strain bearing null mutations in the hexose transporter genes GAL2 and HXT1 to HXT7. Furthermore, its glucose transport activity in this null strain was not markedly different from that of the wild-type Hxt2 protein. We calculated from the fluorescence level and transport kinetics that induced cells had 1.4×105 Hxt2-GFP molecules per cell, and that the catalytic-centre activity of the Hxt2-GFP molecule in vivo is 53 s-1 at 30 °C. Expression of Hxt2-GFP was induced by growth at low concentrations of glucose. Under inducing conditions the Hxt2-GFP fluorescence was localized to the plasma membrane. In a strain impaired in the fusion of secretory vesicles with the plasma membrane, the fluorescence accumulated in the cytoplasm. When induced cells were treated with high concentrations of glucose, the fluorescence was redistributed to the vacuole within 4 h. When endocytosis was genetically blocked, the fluorescence remained in the plasma membrane after treatment with high concentrations of glucose.

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