scholarly journals Single-cell gel electrophoresis assay monitors precise kinetics of DNA fragmentation induced during programmed cell death

Cytometry ◽  
2004 ◽  
Vol 61A (2) ◽  
pp. 127-133 ◽  
Author(s):  
Sudhir Chandna
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Single Cell ◽  
Dna Fragmentation ◽  
Gel Electrophoresis ◽  
Single Cell Gel Electrophoresis ◽  
Kinetics Of

Chromatin supraorganization, DNA fragmentation, and cell death in snake erythrocytes

2005 ◽  
Vol 83 (1) ◽  
pp. 15-27 ◽  
Author(s):  
Maristela Miyamoto ◽  
Benedicto C Vidal ◽  
Maria Luiza S Mello
Keyword(s):  
Cell Death ◽  
Single Cell ◽  
Acid Hydrolysis ◽  
Dna Fragmentation ◽  
Gel Electrophoresis ◽  
Cell Biology ◽  
Tunel Assay ◽  
Snake Species ◽  
Single Cell Gel Electrophoresis ◽  
Bothrops Alternatus

In nucleate erythrocytes of several vertebrate groups, the frequency and intensity of DNA fragmentation associated with programed cell death vary considerably. Although hemoglobin efficiency may be related to erythrocyte life span, and hemoglobin types and erythrocyte life spans are assumed to vary in reptiles, no data on DNA fragmentation and chromatin organization as related to cell death exist for snakes. In the present study, chromatin supraorganization, DNA fragmentation, and cell death were investigated in four snake species (Crotalus durissus terrificus, Bothrops jararaca, Bothrops alternatus, and Bothrops neuwiedii), which differ in their geographical distribution and habitats, by using image analysis of Feulgen hydrolysis kinetics, the TUNEL assay, single-cell gel electrophoresis, and transmission electron microscopy. Relatively few circulating erythrocytes were found to be simultaneously committed to cell death, although there was some variation among the snake species. Conspicuous nuclear and cytoplasmic organelles suggestive of metabolic activity were seen ultrastructurally in most snake erythrocytes. The DNA of the snake erythrocyte chromatin was much more resistant to Feulgen acid hydrolysis (DNA depurination and breakdown) than that of young adult bullfrog erythrocytes, which had a high frequency and intensity of DNA fragmentation. Of the species studied, B. neuwiedii and C. d. terrificus showed the greatest resistance to Feulgen acid hydrolysis and to the DNA fragmentation, revealed by the TUNEL assay. Although B. neuwiedii also showed the lowest frequency of cells with more damaged DNA in the single-cell gel electrophoresis assay, C. d. terrificus had the highest frequency of damaged cells, possibly because of the abundance of alkaline-sensitive DNA sites. The results for DNA fragmentation and cell death in erythrocytes of B. jararaca and B. alternatus generally differed from those for C. d. terrificus and B. neuwiedii and may reflect differences in the biology of these species selected under different geographical habitats. The differences in erythrocyte cell biology reported here may be related to hemoglobin variants selected in the mentioned snake species and that would lead the cells to different resistances to unfavorable environmental conditions.Key words: erythrocytes, snakes, DNA fragmentation, chromatin supraorganization, cell death.

scholarly journals The Effects of Vasectomy on Testicular Tissue of Mice: Histological Changes and DNA Fragmentation Study

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Salman MO ◽  
Al-Wasiti EA ◽  
Thamir KA ◽  
Al-Ani IM ◽  
Al-Salihi AR
Keyword(s):  
Single Cell ◽  
Dna Fragmentation ◽  
Gel Electrophoresis ◽  
Testicular Tissue ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Sham Operation ◽  
Male Mice ◽  
Histological Changes ◽  
Single Cell Gel Electrophoresis

Introduction: We aim to investigate the effect of vasectomy on the histology of the testis as well as to evaluate DNA fragmentation in testicular tissue of male mice. Methods: Bilateral vasectomy was performed on 20 mature male mice; 10 control mice underwent sham-operation. After 6 weeks, the testes were evaluated for histological changes and DNA fragmentation by single cell gel electrophoresis (comet assay). Results: Marked alterations were observed in the testes of vasectomized mice, including degeneration of spermatids, thickened basement membrane, dilatation of the seminiferous tubules, exfoliation of germ cells, reduction in the seminiferous cell population, vacuolated appearance of the epithelium in the tubules and marked interstitial fibrosis. Single cell gel electrophoresis showed a highly significant (P<0.0001) increase in DNA damage among vasectomized mice (46.02%) compared with control group (%27.17) after six weeks of operation. Conclusion: Vasectomy induced deterioration in the seminiferous tubules associated with increased testicular cell’s DNA fragmentation.

Single nucleus versus single-cell gel electrophoresis: Kinetics of DNA track formation

2015 ◽  
Vol 36 (7-8) ◽  
pp. 973-977 ◽  
Author(s):  
Katerina Afanasieva ◽  
Marianna Chopei ◽  
Andrei Sivolob
Keyword(s):  
Single Cell ◽  
Gel Electrophoresis ◽  
Single Cell Gel Electrophoresis ◽  
Track Formation ◽  
Single Nucleus ◽  
Kinetics Of

scholarly journals Intracellular DNA Damage by Lysine-Acetylene Conjugates

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Wang-Yong Yang ◽  
Qiang Cao ◽  
Catherine Callahan ◽  
Catalina Galvis ◽  
Qing-Xiang Sang ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cell Death ◽  
Single Cell ◽  
Cancer Cells ◽  
Anticancer Agents ◽  
Dna Cleavage ◽  
Gel Electrophoresis ◽  
Dna Cleaving ◽  
Single Cell Gel Electrophoresis ◽  
Cells Death

Previously, we reported the design and properties of alkyne C-lysine conjugates, a powerful and tunable family of DNA cleaving reagents. We also reported that, upon photoactivation, these molecules are capable of inducing cancer cells death. To prove that the cell death stems from DNA cleavage by the conjugates, we investigated intracellular DNA damage induced by these molecules in LNCap cancer cells using single cell gel electrophoresis (SCGE) assays. The observation of highly efficient DNA damage confirmed that lysine acetylene conjugate is capable of cleaving the densely compacted intracellular DNA. This result provides a key mechanistic link between efficient DNA cleavage and cytotoxicity towards cancer cells for this family of light-activated anticancer agents.

scholarly journals Influence of chloroquine on kinetics of single-cell gel electrophoresis

2012 ◽  
Vol 28 (4) ◽  
pp. 292-297 ◽  
Author(s):  
M. O. Zazhytska ◽  
K. S. Afanasieva ◽  
M. I. Chopei ◽  
M. A. Vikhreva ◽  
A. V. Sivolob
Keyword(s):  
Single Cell ◽  
Gel Electrophoresis ◽  
Single Cell Gel Electrophoresis ◽  
Kinetics Of

Kinetics of comet formation in single-cell gel electrophoresis: Loops and fragments

2010 ◽  
Vol 31 (3) ◽  
pp. 512-519 ◽  
Author(s):  
Katerina Afanasieva ◽  
Marianna Zazhytska ◽  
Andrei Sivolob
Keyword(s):  
Single Cell ◽  
Gel Electrophoresis ◽  
Single Cell Gel Electrophoresis ◽  
Kinetics Of
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