scholarly journals Impact of flow cytometry on the diagnosis and characterization of lymphomas, chronic lymphoproliferative disorders and plasma cell neoplasias

Cytometry ◽  
2004 ◽  
Vol 58A (1) ◽  
pp. 57-61 ◽  
Author(s):  
Raul C. Braylan
Keyword(s):  
Flow Cytometry ◽  
Plasma Cell ◽  
Lymphoproliferative Disorders

Impact of Flow Cytometry in Liver Cytopathology

2015 ◽  
Vol 59 (1) ◽  
pp. 51-60 ◽  
Author(s):  
Jennifer P. Bynum ◽  
Amy Duffield ◽  
Syed Z. Ali
Keyword(s):  
Flow Cytometry ◽  
Fine Needle Aspiration ◽  
Diagnostic Tool ◽  
Needle Aspiration ◽  
Lymphoproliferative Disorders ◽  
Johns Hopkins Hospital ◽  
Lymphoproliferative Diseases ◽  
Fine Needle ◽  
Negative Results

Background: Fine needle aspiration (FNA) is commonly used as a diagnostic tool for the evaluation of lymphoproliferative diseases. Cytomorphology alone is often insufficient for the diagnosis and subclassification of lymphoma; therefore, flow cytometry (FC) plays an important role in the characterization of lymphoproliferative disorders. This study reviews our experience with FC on liver FNA at the Johns Hopkins Hospital. Methods: 2,424 liver FNAs performed over a 21-year period were reviewed for clinical FC data (n = 74) or a subsequent diagnosis of lymphoma in the liver without FC data (n = 40). Results: In our study, 114 cases (4.7%) were included out of the 2,424 liver FNAs performed during the study period. Lymphoma was diagnosed 79 times. Cytomorphology alone was diagnostic of lymphoma in 45 cases, and in 33 cases both the cytomorphology and the FC were consistent with a diagnosis of lymphoma. Neither FC nor cytomorphology were diagnostic of lymphoma on 1 specimen. In 39 cases, FC had negative results on a lesion suspicious for lymphoma based on cytomorphology. In several nonlymphoma cases, FC provided information that allowed further subclassification of the neoplasm. Conclusion: FC is a useful adjuvant diagnostic test for liver FNAs performed on patients with lymphoproliferative disorders.

scholarly journals Cytogenetic and therapeutic characterization of primary plasma cell leukemia: the IFM experience

Leukemia ◽  
2011 ◽  
Vol 26 (1) ◽  
pp. 158-159 ◽  
Author(s):  
H Avet-Loiseau ◽  
M Roussel ◽  
L Campion ◽  
X Leleu ◽  
G Marit ◽  
...  
Keyword(s):  
Plasma Cell ◽  
Plasma Cell Leukemia ◽  
Cell Leukemia ◽  
Primary Plasma Cell Leukemia

scholarly journals KD determination from time-resolved experiments on live cells with LigandTracer and reconciliation with end-point flow cytometry measurements

2021 ◽  
Author(s):  
Diana Spiegelberg ◽  
Jonas Stenberg ◽  
Pascale Richalet ◽  
Marc Vanhove
Keyword(s):  
Flow Cytometry ◽  
Live Cells ◽  
Time Resolved ◽  
Surface Receptors ◽  
Full Characterization ◽  
End Point ◽  
Titration Curves ◽  
Kinetics Of

AbstractDesign of next-generation therapeutics comes with new challenges and emulates technology and methods to meet them. Characterizing the binding of either natural ligands or therapeutic proteins to cell-surface receptors, for which relevant recombinant versions may not exist, represents one of these challenges. Here we report the characterization of the interaction of five different antibody therapeutics (Trastuzumab, Rituximab, Panitumumab, Pertuzumab, and Cetuximab) with their cognate target receptors using LigandTracer. The method offers the advantage of being performed on live cells, alleviating the need for a recombinant source of the receptor. Furthermore, time-resolved measurements, in addition to allowing the determination of the affinity of the studied drug to its target, give access to the binding kinetics thereby providing a full characterization of the system. In this study, we also compared time-resolved LigandTracer data with end-point KD determination from flow cytometry experiments and hypothesize that discrepancies between these two approaches, when they exist, generally come from flow cytometry titration curves being acquired prior to full equilibration of the system. Our data, however, show that knowledge of the kinetics of the interaction allows to reconcile the data obtained by flow cytometry and LigandTracer and demonstrate the complementarity of these two methods.

scholarly journals Limitations of VS38c labeling in the detection of plasma cell myeloma by flow cytometry

2021 ◽  
Author(s):  
Ágnes Czeti ◽  
Gábor Szalóki ◽  
Gergely Varga ◽  
Virág Réka Szita ◽  
Zsolt István Komlósi ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Plasma Cell ◽  
Plasma Cell Myeloma

Polarized light-scattering profile-advanced characterization of nonspherical particles with scanning flow cytometry

2011 ◽  
Vol 79A (7) ◽  
pp. 570-579 ◽  
Author(s):  
Dmitry I. Strokotov ◽  
Alexander E. Moskalensky ◽  
Vyacheslav M. Nekrasov ◽  
Valeri P. Maltsev
Keyword(s):  
Flow Cytometry ◽  
Light Scattering ◽  
Polarized Light ◽  
Advanced Characterization ◽  
Nonspherical Particles

T2050 Immunophenotypic Characterization of the Cellular Infiltrate During Murine Experimental Colitis Using Multiparameter Flow Cytometry

2010 ◽  
Vol 138 (5) ◽  
pp. S-621
Author(s):  
Kimberly A. Zins ◽  
Tamas Ordog ◽  
Michael R. Bardsley ◽  
Gianrico Farrugia ◽  
Joseph H. Szurszewski ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Experimental Colitis ◽  
Multiparameter Flow Cytometry ◽  
Cellular Infiltrate

Characterization of the tumor microenvironment in primary cutaneous CD30-positive lymphoproliferative disorders: a predominance of CD163-positive M2 macrophages

2016 ◽  
Vol 43 (7) ◽  
pp. 579-588 ◽  
Author(s):  
Aieska De Souza ◽  
Marianne Tinguely ◽  
Daniel R. Burghart ◽  
Arbeneshe Berisha ◽  
Kirsten D. Mertz ◽  
...  
Keyword(s):  
Tumor Microenvironment ◽  
Lymphoproliferative Disorders ◽  
M2 Macrophages

Characterization of antigen-presenting cells in human apical periodontitis lesions by flow cytometry and immunocytochemistry

2006 ◽  
Vol 39 (8) ◽  
pp. 626-636 ◽  
Author(s):  
A. Lukic ◽  
S. Vasilijic ◽  
I. Majstorovic ◽  
D. Vucevic ◽  
S. Mojsilovic ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Antigen Presenting Cells ◽  
Apical Periodontitis ◽  
Antigen Presenting
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