scholarly journals Reevaluation of flow cytometry for investigating antibody binding to the surface ofPlasmodium falciparum trophozoite-infected red blood cells

Cytometry ◽  
2003 ◽  
Vol 56A (2) ◽  
pp. 96-103 ◽  
Author(s):  
T. N. Williams ◽  
C. I. Newbold
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Antibody Binding ◽  
Ofplasmodium Falciparum

scholarly journals Indicators of Erythrocyte Damage after Microwave Warming of Packed Red Blood Cells

2003 ◽  
Vol 49 (5) ◽  
pp. 792-799 ◽  
Author(s):  
Jan Hirsch ◽  
Axel Menzebach ◽  
Ingeborg Dorothea Welters ◽  
Gerald Volker Dietrich ◽  
Norbert Katz ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Room Temperature ◽  
Antibody Binding ◽  
Cellular Damage ◽  
Substantial Part ◽  
Packed Red Blood Cells ◽  
Before And After ◽  
Cellular Markers

Abstract Background: Localized overheating of packed red blood cells (PRBCs) after microwave warming with consequent damage to erythrocytes has been reported. We therefore compared possible cellular markers of erythrocyte damage, as measured by flow cytometry, with laboratory indicators of hemolysis to evaluate the effects of microwave warming on PRBCs. Methods: PRBC samples were warmed to room temperature or to 37, 42, 47, 52, or 57 °C in a water bath. Flow cytometry was performed after fluorescein labeling using antibodies to spectrin, Ca2+-ATPase, and Na+-K+-ATPase. The forward-to-sideward scatter (FSC/SSC) ratio and antibody binding were evaluated. Plasma free hemoglobin (FHb) and α-hydroxybutyrate dehydrogenase (HBDH) were measured immediately after heating and after 48 h. In addition, all measurements were made before and after the heating of PRBCs to 35 °C by a microwave blood warmer. Results: Analysis of 15 000 erythrocytes showed a decrease in the FSC/SSC ratio and antibody binding above 47 °C [at 37 °C, median (SD) of 94.2 (7.4) with 0.07 (0.05)% fluorescein-positive; at 52 °C, median (SD) of 177.0 (19.0) with 18.5 (6.4)% positively gated; P <0.001]. FHb [room temperature, 0.3 (0.2) g/L] was increased 2-fold at 37 and 42 °C, 4-fold at 47 °C, and 25-fold at 52 °C. HBDH increased in parallel. Hemolysis markers showed an additional twofold increase 48 h after heating to 42 and 47 °C. Microwave heating to 35 °C did not produce significant changes of any marker. Conclusions: All markers of cellular damage were altered after heating to >47 °C, and a substantial part of hemolysis was delayed. The methodology can be used for future testing of other blood warming devices.

Microparticles in stored red blood cells: an approach using flow cytometry and proteomic tools

Vox Sanguinis ◽  
2008 ◽  
Vol 95 (4) ◽  
pp. 288-297 ◽  
Author(s):  
O. Rubin ◽  
D. Crettaz ◽  
G. Canellini ◽  
J.-D. Tissot ◽  
N. Lion
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Blood Cells

Improved quantitative detection of biotin‐labeled red blood cells by flow cytometry

Transfusion ◽  
2019 ◽  
Vol 59 (8) ◽  
pp. 2691-2698 ◽  
Author(s):  
Albert D. Donnenberg ◽  
Tamir Kanias ◽  
Darrell J. Triulzi ◽  
Catherine J. Dennis ◽  
E. Michael Meyer ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Quantitative Detection

High-resolution X-ray imaging ofPlasmodium falciparum-infected red blood cells

2008 ◽  
Vol 73A (10) ◽  
pp. 949-957 ◽  
Author(s):  
Garth J. Williams ◽  
Eric Hanssen ◽  
Andrew G. Peele ◽  
Mark A. Pfeifer ◽  
Jesse Clark ◽  
...  
Keyword(s):  
High Resolution ◽  
Red Blood Cells ◽  
Blood Cells ◽  
X Ray ◽  
X Ray Imaging ◽  
Ofplasmodium Falciparum

scholarly journals Assessment of deformation of human red blood cells in flow cytometry: measurement and simulation of bimodal forward scatter distributions

2019 ◽  
Vol 10 (9) ◽  
pp. 4531
Author(s):  
Jonas Gienger ◽  
Hermann Gross ◽  
Volker Ost ◽  
Markus Bär ◽  
Jörg Neukammer
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Forward Scatter ◽  
Human Red Blood Cells

Trauma and Pregnancy: Is Flow Cytometry Detection and Quantification of Fetal Red Blood Cells Useful? A Retrospective Cohort Study.

2021 ◽  
Author(s):  
Misgav Rottenstreich ◽  
Reut Meir ◽  
Itamar Glick ◽  
Heli Alexandrony ◽  
Alon D Schwarz ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cohort Study ◽  
Red Blood Cells ◽  
Pregnant Women ◽  
Retrospective Cohort Study ◽  
Blood Cells ◽  
Retrospective Cohort ◽  
Neonatal Outcome ◽  
Adverse Outcomes ◽  
Minor Trauma

Abstract Purpose: To assess whether positive flow cytometry quantification of fetal red blood cells is associated with adverse outcomes in cases of trauma during pregnancy.Methods: A retrospective cohort study, at a single tertiary center between 2013 and 2019. All pregnant women with viable gestation involved in trauma who underwent flow cytometry were included. Flow cytometry was considered positive (≥0.03/≥30 ml). Composite adverse maternal and neonatal outcomes were compared between cases with positive and negative flow cytometry test. Univariate and multivariate logistic regression analysis were performed to assess the role of flow cytometry in predicting adverse outcomes. Adjusted odds ratios (aORs) and 95% confidence intervals (CIs) were calculated.Results: During the study 1023 women met inclusion and exclusion criteria. The mechanisms of injury were motor vehicle accident in 387 women (38%), falls in 367 (36%), direct abdominal trauma in 353 (35%) and in 14 women (1%) other mechanism of injury. Among the cohort, 119 women (11.6%) had positive flow cytometry (≥0.03/≥30 ml) with median result of 0.03 [0.03-0.04], while 904 women (88.4%) had negative flow cytometry test result (≤0.03/≤30 ml) with median result of 0.01 [0.01-0.02]. Composite adverse outcome occurred in 8% of the women, with no difference in the groups with vs. without positive flow cytometry (4.2% vs. 8.5%; p=0.1). Positive flow cytometry was not associated with any adverse maternal or neonatal outcome. This was confirmed on a multivariate analysis. Conclusions: Flow cytometry result is not related to adverse maternal and fetal/neonatal outcome of women involved in minor trauma during pregnancy. We suggest that flow cytometry should not be routinely assessed in pregnant women involved in minor trauma.

The Role of Lactadherin in the Phagocytosis of Phosphatidylserine-Expressing Sickle Red Blood Cell by Macrophages.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3773-3773
Author(s):  
Swapan K. Dasgupta ◽  
Perumal Thiagarajan
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Sickle Cell ◽  
Blood Cells ◽  
Milk Fat ◽  
Integrin Αvβ3 ◽  
Fold Increase ◽  
Anionic Phospholipid ◽  
Sickle Red Blood Cells

Abstract Sickle cell anemia, the most common serious hemoglobinopathy, is associated with a markedly reduced life span of red blood cells due to their preferential clearance by macrophages. During polymerization of sickle hemoglobin, phosphatidylserine, an anionic phospholipid normally present exclusively on the inner leaflet of the membrane bilayer is exteriorized to outer leaflet. This exposure of phosphatidylserine is thought to be a tag for macrophage recognition. Lactadherin, also known as milk fat globule-EGF factor 8, is a phosphatidylserine-binding glycoprotein secreted by macrophages that promotes the engulfment of apoptotic cells. Here, we investigated the role of lactadherin in the phagocytosis of sickle red blood cells. The binding of fluorescein-lactadherin to normal and sickle red blood cells was studied by flow cytometry. We quantified the effect of lactadherin on phagocytosis of red blood cells by monocyte-derived macrophage. In normal individuals, less than 0.5% of red blood cells showed any binding to lactadherin when analyzed by flow cytometry. However, in sickle cell patients, circulating red blood cells showed 2 to 10- fold increase in lactadherin binding (P<0.0002). Lactadherin stimulated the phagocytosis of resting sickle red blood cells by macrophages but had no significant effect on the phagocytosis of normal red blood cells. Deoxygenation of sickle red blood cells further increased the lactadherin binding and phagocytosis. Antibodies to integrin αVβ3 also inhibited macrophage binding and phagocytosis. These results show lactadherin may play a major role in sickle red cell clearance by anchoring the phosphatidylserine-expressing sickle red blood cells to integrins on tissue macrophages.

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