In vitro model of “wound healing” analyzed by laser scanning cytometry: Accelerated healing of epithelial cell monolayers in the presence of hyaluronate

Cytometry ◽  
2003 ◽  
Vol 53A (1) ◽  
pp. 1-8 ◽  
Author(s):  
Asifa S. Haider ◽  
Jerzy Grabarek ◽  
Ben Eng ◽  
Paulina Pedraza ◽  
Nicholas R. Ferreri ◽  
...  
Keyword(s):  
Wound Healing ◽  
Epithelial Cell ◽  
Laser Scanning ◽  
In Vitro Model ◽  
Cell Monolayers ◽  
Laser Scanning Cytometry ◽  
Epithelial Cell Monolayers ◽  
Vitro Model

An in vitro Model of Pseudomonas aeruginosa Biofilms on Viable Airway Epithelial Cell Monolayers

2008 ◽  
Vol 22 (3) ◽  
pp. 235-238 ◽  
Author(s):  
Bradford A. Woodworth ◽  
Edwin Tamashiro ◽  
Geeta Bhargave ◽  
Noam A. Cohen ◽  
James N. Palmer
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Epithelial Cell ◽  
In Vitro Model ◽  
Airway Epithelial Cell ◽  
Airway Epithelial ◽  
Cell Monolayers ◽  
Epithelial Cell Monolayers ◽  
Vitro Model

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

scholarly journals A Novel Pharmacological Approach to Enhance the Integrity and Accelerate Restitution of the Intestinal Epithelial Barrier

2020 ◽  
Vol 26 (9) ◽  
pp. 1340-1352
Author(s):  
Xuelei Cao ◽  
Lei Sun ◽  
Susana Lechuga ◽  
Nayden G Naydenov ◽  
Alex Feygin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Epithelial Cell ◽  
Epithelial Barrier ◽  
Intestinal Epithelial ◽  
Intestinal Epithelial Barrier ◽  
Cell Monolayers ◽  
Barrier Disruption ◽  
Epithelial Cell Monolayers ◽  
Immunofluorescence Labeling ◽  
E Cadherin

Abstract Background Disruption of the gut barrier is an essential mechanism of inflammatory bowel diseases (IBDs) contributing to the development of mucosal inflammation. A hallmark of barrier disruption is the disassembly of epithelial adherens junctions (AJs) driven by decreased expression of a major AJ protein, E-cadherin. A group of isoxazole compounds, such as E-cadherin-upregulator (ECU) and ML327, were previously shown to stimulate E-cadherin expression in poorly differentiated human cancer cells. This study was designed to examine whether these isoxazole compounds can enhance and protect model intestinal epithelial barriers in vitro. Methods The study was conducted using T84, SK-CO15, and HT-29 human colonic epithelial cell monolayers. Disruption of the epithelial barrier was induced by pro-inflammatory cytokines, tumor necrosis factor-α, and interferon-γ. Barrier integrity and epithelial junction assembly was examined using different permeability assays, immunofluorescence labeling, and confocal microscopy. Epithelial restitution was analyzed using a scratch wound healing assay. Results E-cadherin-upregulator and ML327 treatment of intestinal epithelial cell monolayers resulted in several barrier-protective effects, including reduced steady-state epithelial permeability, inhibition of cytokine-induced barrier disruption and junction disassembly, and acceleration of epithelial wound healing. Surprisingly, these effects were not due to upregulation of E-cadherin expression but were mediated by multiple mechanisms including inhibition of junction protein endocytosis, attenuation of cytokine-induced apoptosis, and activation of promigratory Src and AKT signaling. Conclusions Our data highlight ECU and ML327 as promising compounds for developing new therapeutic strategies to protect the integrity and accelerate the restitution of the intestinal epithelial barrier in IBD and other inflammatory disorders.

099 "Smart" Targeting of Anti?Scarring Agents in an in vitro Model of Retinal Wound Healing

2004 ◽  
Vol 12 (2) ◽  
pp. A26-A26
Author(s):  
F E Dhawahir ◽  
C Sheridan ◽  
D Kent ◽  
D Wong ◽  
I Grierson ◽  
...  
Keyword(s):  
Wound Healing ◽  
In Vitro Model ◽  
Vitro Model

Kidney tubular epithelial cell injury in shock wave lithoripsy: The search for an appropriate in vitro model

1995 ◽  
Vol 98 (5) ◽  
pp. 2942-2943
Author(s):  
James A. McAteer ◽  
Andrew P. Evan ◽  
James E. Lingeman ◽  
Sharon P. Andreoli
Keyword(s):  
Shock Wave ◽  
Epithelial Cell ◽  
In Vitro Model ◽  
Cell Injury ◽  
Tubular Epithelial Cell ◽  
Vitro Model ◽  
Epithelial Cell Injury
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