scholarly journals Chronology of cellular alterations during 7-ketocholesterol-induced cell death on A7R5 rat smooth muscle cells: Analysis by time lapse-video microscopy and conventional fluorescence microscopy

2003 ◽  
Vol 52A (2) ◽  
pp. 57-69 ◽  
Author(s):  
Jean-Marie Zahm ◽  
Sonia Baconnais ◽  
Serge Monier ◽  
Noël Bonnet ◽  
Ginette Bessède ◽  
...  
Keyword(s):  
Cell Death ◽  
Smooth Muscle ◽  
Fluorescence Microscopy ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Time Lapse ◽  
Video Microscopy ◽  
Conventional Fluorescence Microscopy ◽  
Cellular Alterations ◽  
Time Lapse Video

scholarly journals Antioxidant Properties of Olive Mill Wastewater Polyphenolic Extracts on Human Endothelial and Vascular Smooth Muscle Cells

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 800
Author(s):  
Anna Maria Posadino ◽  
Annalisa Cossu ◽  
Roberta Giordo ◽  
Amalia Piscopo ◽  
Wael M Abdel-Rahman ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cell Death ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Olive Oil ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Olive Mill Wastewater ◽  
Olive Mill

This work aims to analyze the chemical and biological evaluation of two extracts obtained by olive mill wastewater (OMW), an olive oil processing byproduct. The exploitation of OMW is becoming an important aspect of development of the sustainable olive oil industry. Here we chemically and biologically evaluated one liquid (L) and one solid (S) extract obtained by liquid–liquid extraction followed by acidic hydrolysis (LLAC). Chemical characterization of the two extracts indicated that S has higher phenol content than L. Hydroxytyrosol and tyrosol were the more abundant phenols in both OMW extracts, with hydroxytyrosol significantly higher in S as compared to L. Both extracts failed to induce cell death when challenged with endothelial cells and vascular smooth muscle cells in cell viability experiments. On the contrary, the higher extract dosages employed significantly affected cell metabolic activity, as indicated by the MTT tests. Their ability to counteract H2O2-induced oxidative stress and cell death was assessed to investigate potential antioxidant activities of the extracts. Fluorescence measurements obtained with the reactive oxygen species (ROS) probe H2DCF-DA indicated strong antioxidant activity of the two OMW extracts in both cell models, as indicated by the inhibition of H2O2-induced ROS generation and the counteraction of the oxidative-induced cell death. Our results indicate LLAC-obtained OMW extracts as a safe and useful source of valuable compounds harboring antioxidant activity.

scholarly journals Calcium Phosphate Crystals Induce Cell Death in Human Vascular Smooth Muscle Cells

2008 ◽  
Vol 103 (5) ◽  
Author(s):  
Alexandra E. Ewence ◽  
Martin Bootman ◽  
H. Llewelyn Roderick ◽  
Jeremy N. Skepper ◽  
Geraldine McCarthy ◽  
...  
Keyword(s):  
Cell Death ◽  
Smooth Muscle ◽  
Calcium Phosphate ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Induce Cell Death

Fas/Fas ligand-mediated death pathway is involved in oxLDL-induced apoptosis in vascular smooth muscle cells

2001 ◽  
Vol 280 (3) ◽  
pp. C709-C718 ◽  
Author(s):  
Tzong-Shyuan Lee ◽  
Lee-Young Chau
Keyword(s):  
Cell Death ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Fas Ligand ◽  
Density Lipoprotein ◽  
Muscle Cells ◽  
Potent Inducer ◽  
Apoptotic Death

Oxidized low-density lipoprotein (oxLDL) is a potent inducer of apoptosis for vascular cells. In the present study, we demonstrate that the expression of death mediators, including p53, Fas, and Fas ligand (FasL) was substantially upregulated by oxLDL in cultured vascular smooth muscle cells (SMCs). The induction of these death mediators was time dependent and was accompanied by an increase in apoptotic death of SMCs following oxLDL treatment. Two oxysterols, 7β-hydroxycholesterol and 25-hydroxycholesterol, were also effective to induce the expression of death mediators and apoptosis. α-Tocopherol and deferoxamine significantly attenuated the induction of death mediators and cell death induced by oxLDL and oxysterols, suggesting that reactive oxygen species are involved in triggering the apoptotic event. Incubation of cells with FasL-neutralizing antibody inhibited the oxLDL-induced cell death up to 50%. Furthermore, caspase 8 and caspase 3 activities were induced time dependently in SMCs following oxLDL treatment. Collectively, these data suggest that the Fas/FasL death pathway is activated and responsible for, at least in part, the apoptotic death in vascular SMCs upon exposure to oxLDL.

Endothelin-1 Enhances Nitric Oxide-Induced Cell Death in Cultured Vascular Smooth-Muscle Cells

1998 ◽  
Vol 31 ◽  
pp. S351-S353 ◽  
Author(s):  
Takeshi Nakahashi ◽  
Keisuke Fukuo ◽  
Hiroyuki Nishimaki ◽  
Shigeki Hata ◽  
Masumi Shimizu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Death ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Endothelin 1

scholarly journals High glucose induces cell death of cultured human aortic smooth muscle cells through the formation of hydrogen peroxide

2001 ◽  
Vol 133 (7) ◽  
pp. 967-974 ◽  
Author(s):  
Concepción Peiró ◽  
Nuria Lafuente ◽  
Nuria Matesanz ◽  
Elena Cercas ◽  
José L Llergo ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Death ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
High Glucose ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle

scholarly journals Chemokines protect vascular smooth muscle cells from cell death induced by cyclic mechanical stretch

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Jing Zhao ◽  
Yuhei Nishimura ◽  
Akihiko Kimura ◽  
Kentaro Ozawa ◽  
Toshikazu Kondo ◽  
...  
Keyword(s):  
Cell Death ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Mechanical Stretch
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