scholarly journals Fluorescence lifetime-based discrimination and quantification of cellular DNA and RNA with phase-sensitive flow cytometry

Cytometry ◽  
2003 ◽  
Vol 52A (1) ◽  
pp. 46-55 ◽  
Author(s):  
H. Helen Cui ◽  
Joseph G. Valdez ◽  
John A. Steinkamp ◽  
Harry A. Crissman
Keyword(s):  
Flow Cytometry ◽  
Fluorescence Lifetime ◽  
Dna And Rna ◽  
Phase Sensitive ◽  
Cellular Dna

Analysis of the cellular DNA and RNA content in acute leukemias by flow cytometry

1990 ◽  
Vol 116 (5) ◽  
pp. 507-512 ◽  
Author(s):  
W. Hiddemann ◽  
B. Wörmann ◽  
D. Messerer ◽  
R. Springefeld ◽  
Th. Büchner
Keyword(s):  
Flow Cytometry ◽  
Acute Leukemias ◽  
Dna And Rna ◽  
Rna Content ◽  
Cellular Dna

scholarly journals Improved flow cytometry of cellular DNA and RNA by on-line reagent addition

Cytometry ◽  
1987 ◽  
Vol 8 (3) ◽  
pp. 335-338 ◽  
Author(s):  
Arie Pennings ◽  
Paul Speth ◽  
Hans Wessels ◽  
Clemens Haanen
Keyword(s):  
Flow Cytometry ◽  
Dna And Rna ◽  
On Line ◽  
Cellular Dna

Analysis of fluorescence lifetime and quenching of FITC-conjugated antibodies on cells by phase-sensitive flow cytometry

Cytometry ◽  
1996 ◽  
Vol 25 (3) ◽  
pp. 271-279 ◽  
Author(s):  
C. Deka ◽  
B. E. Lehnert ◽  
N. M. Lehnert ◽  
G. M. Jones ◽  
L. A. Sklar ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Fluorescence Lifetime ◽  
Phase Sensitive

scholarly journals Subcellular localization-dependent changes in EGFP fluorescence lifetime measured by time-resolved flow cytometry

2013 ◽  
Vol 4 (8) ◽  
pp. 1390 ◽  
Author(s):  
Ali Vaziri Gohar ◽  
Ruofan Cao ◽  
Patrick Jenkins ◽  
Wenyan Li ◽  
Jessica P. Houston ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Subcellular Localization ◽  
Fluorescence Lifetime ◽  
Time Resolved ◽  
Egfp Fluorescence

Human RecQ helicases in transcription-associated stress management: bridging the gap between DNA and RNA metabolism

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Tulika Das ◽  
Surasree Pal ◽  
Agneyo Ganguly
Keyword(s):  
Genome Integrity ◽  
Complex Structures ◽  
Huge Number ◽  
Dna Helicases ◽  
Dna Structures ◽  
Recq Helicases ◽  
Dna And Rna ◽  
Cellular Dna ◽  
Almost All ◽  
Transcription And Replication

Abstract RecQ helicases are a highly conserved class of DNA helicases that play crucial role in almost all DNA metabolic processes including replication, repair and recombination. They are able to unwind a wide variety of complex intermediate DNA structures that may result from cellular DNA transactions and hence assist in maintaining genome integrity. Interestingly, a huge number of recent reports suggest that many of the RecQ family helicases are directly or indirectly involved in regulating transcription and gene expression. On one hand, they can remove complex structures like R-loops, G-quadruplexes or RNA:DNA hybrids formed at the intersection of transcription and replication. On the other hand, emerging evidence suggests that they can also regulate transcription by directly interacting with RNA polymerase or recruiting other protein factors that may regulate transcription. This review summarizes the up to date knowledge on the involvement of three human RecQ family proteins BLM, WRN and RECQL5 in transcription regulation and management of transcription associated stress.

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