scholarly journals Scanning microspectrofluorometry of rhodamine 123 in multidrug-resistant cells

Cytometry ◽  
1994 ◽  
Vol 17 (1) ◽  
pp. 50-58 ◽  
Author(s):  
Jean-Marc Millot ◽  
Sergueï Sharonov ◽  
Michel Manfait
Keyword(s):  
Multidrug Resistant ◽  
Rhodamine 123 ◽  
Resistant Cells

Heparin reverses Rhodamine 123 extrusion by multidrug resistant cells

1996 ◽  
Vol 106 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Raquel C. Maia ◽  
Karen Wagner ◽  
Ramza H. Cabral ◽  
Vivian M. Rumjanek
Keyword(s):  
Multidrug Resistant ◽  
Rhodamine 123 ◽  
Resistant Cells

Laser scanning and confocal microscopy of daunorubicin, doxorubicin, and rhodamine 123 in multidrug-resistant cells

1991 ◽  
Vol 196 (2) ◽  
pp. 323-329 ◽  
Author(s):  
James L. Weaver ◽  
P.Scott Pine ◽  
Adorjan Aszalos ◽  
Patricia V. Schoenlein ◽  
Stephen J. Currier ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Laser Scanning ◽  
Multidrug Resistant ◽  
Rhodamine 123 ◽  
Resistant Cells

USE OF NONYL ACRIDINE ORANGE AND RHODAMINE 123 TO ASSESS STRUCTURAL AND FUNCTIONAL MODIFICATIONS OF MITOCHONDRIA IN MULTIDRUG RESISTANT CELLS

1991 ◽  
Vol 73 (2-3) ◽  
pp. 48a-48a
Author(s):  
Michelle Denis-Gay ◽  
Abderrahman Maftah ◽  
Marie-Hélène Ratinaud ◽  
Raymond Julien
Keyword(s):  
Acridine Orange ◽  
Multidrug Resistant ◽  
Rhodamine 123 ◽  
Resistant Cells

scholarly journals Reversal of Multidrug Resistance in Human Colon Cancer and Human Leukemia Cells by Three Plant Extracts and Their Major Secondary Metabolites

Medicines ◽  
2018 ◽  
Vol 5 (4) ◽  
pp. 123 ◽  
Author(s):  
Jun-Xian Zhou ◽  
Michael Wink
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Abc Transporters ◽  
Human Colon ◽  
Rhodamine 123 ◽  
Human Leukemia ◽  
Dependent Manner ◽  
Human Colon Cancer ◽  
High Concentrations ◽  
Resistant Cells

Background: We studied the effect of three plant extracts (Glycyrrhiza glabra, Paeonia lactiflora, Eriobotrya japonica) and six of their major secondary metabolites (glycyrrhizic acid, 18β glycyrrhetinic acid, liquiritigenin, isoliquiritigenin, paeoniflorin, ursolic acid) on the multidrug resistant human colon cancer cell line Caco-2 and human leukemia cell line CEM/ADR 5000 as compared to the corresponding sensitive cell line CCRF-CEM, and human colon cancer cells HCT-116, which do not over-express ATP-binding cassette (ABC) transporters. Methods: The cytotoxicity of single substances in sensitive and resistant cells was investigated by MTT assay. We also applied combinations of extracts or single compounds with the chemotherapeutic agent doxorubicin or doxorubicin plus the saponin digitonin. The intracellular retention of the ABC transporter substrates rhodamine 123 and calcein was examined by flow cytometry to explore the effect of the substances on the activity of ABC transporters P-glycoprotein and MRP1. Real-time PCR was applied to analyse the gene expression changes of ABCB1, ABCC1, caspase 3, caspase 8, AhR, CYP1A1, and GSTP1 in resistant cells under the treatment of the substances. Results: All the substances moderately inhibited cell growth in sensitive and resistant cells to some degree. Whereas ursolic acid showed IC50 of 14 and 22 µM in CEM/ADR 5000 and Caco-2 cells, respectively, glycyrrhizic acid and paeoniflorin were inactive with IC50 values above 400 μM. Except for liquiritigenin and isoliquiritigenin, all the other substances reversed MDR in CEM/ADR 5000 and Caco-2 cells to doxorubicin. Ue, ga, 18ga, and urs were powerful reversal agents. In CEM/ADR 5000 cells, high concentrations of all the substances, except Paeonia lactiflora extract, increased calcein or rhodamine 123 retention in a dose-dependent manner. In Caco-2 cells, all the substances, except liquiritigenin, retained rhodamine 123 in a dose-dependent manner. We also examined the effect of the plant secondary metabolite (PSM) panel on the expression of ABCB1, ABCC1, caspase 3, caspase 8, AhR, CYP1A1, and GSTP1 genes in MDR cells. Conclusions: The extracts and individual PSM could reverse MDR in CEM/ADR 5000 and Caco-2 cells, which overexpress ABC transporters, in two- and three-drug combinations. Most of the PSM also inhibited the activity of ABC transporters to some degree, albeit at high concentrations. Ue, ga, 18ga, and urs were identified as potential multidrug resistance (MDR) modulator candidates, which need to be characterized and validated in further studies.

scholarly journals Transcriptional regulation of MDR-1 by HOXC6 in multidrug-resistant cells

Oncogene ◽  
2012 ◽  
Vol 32 (28) ◽  
pp. 3339-3349 ◽  
Author(s):  
K-J Kim ◽  
S-M Moon ◽  
S-A Kim ◽  
K-W Kang ◽  
J-H Yoon ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Multidrug Resistant ◽  
Mdr 1 ◽  
Resistant Cells
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