scholarly journals BrdUrd/DNA flow cytometry analyses demonstrates cis-diamminedichloroplatinum (II)-induced multiple cell-cycle modifications on human lung carcinoma cells

Cytometry ◽  
1992 ◽  
Vol 13 (4) ◽  
pp. 416-422 ◽  
Author(s):  
Catherine Demarcq ◽  
Gérard Bastian ◽  
Yorghos Remvikos
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Lung Carcinoma ◽  
Human Lung ◽  
Carcinoma Cells ◽  
Dna Flow Cytometry ◽  
Lung Carcinoma Cells ◽  
Human Lung Carcinoma ◽  
Multiple Cell

Induction of Cell Cycle Arrest and Apoptosis by Ruthenium Complex cis-(Dichloro)tetramineruthenium(III) Chloride in Human Lung Carcinoma Cells A549

2011 ◽  
Vol 147 (1-3) ◽  
pp. 8-15 ◽  
Author(s):  
Aliny Pereira de Lima ◽  
Flávia de Castro Pereira ◽  
Cesar Augusto Sam Tiago Vilanova-Costa ◽  
Jordana Ribeiro Soares ◽  
Lucas Carlos Gomes Pereira ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Lung Carcinoma ◽  
Human Lung ◽  
Ruthenium Complex ◽  
Carcinoma Cells ◽  
Lung Carcinoma Cells ◽  
Cycle Arrest ◽  
Human Lung Carcinoma

scholarly journals Effect of an hdm-2 antagonist peptide inhibitor on cell cycle progression in p53-deficient H1299 human lung carcinoma cells

Oncogene ◽  
2006 ◽  
Vol 25 (50) ◽  
pp. 6672-6677 ◽  
Author(s):  
A VanderBorght ◽  
A Valckx ◽  
J Van Dun ◽  
T Grand-Perret ◽  
S De Schepper ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Lung Carcinoma ◽  
Cell Cycle Progression ◽  
Human Lung ◽  
Carcinoma Cells ◽  
Peptide Inhibitor ◽  
Cycle Progression ◽  
Lung Carcinoma Cells ◽  
Human Lung Carcinoma

scholarly journals Serum deprivation enhanced ethanol-induced toxic responses in A549, lung carcinoma cells

2022 ◽  
Vol 43 (1) ◽  
pp. 26-34
Author(s):  
P. Khare ◽  
◽  
V.K. Singh ◽  
L. Bala ◽  
◽  
...  
Keyword(s):  
Cell Cycle ◽  
Lung Carcinoma ◽  
Human Lung ◽  
Ethanol Concentration ◽  
Carcinoma Cells ◽  
Complete Medium ◽  
Lung Carcinoma Cells ◽  
Cycle Arrest ◽  
Human Lung Carcinoma ◽  
A549 Lung

Aim: The current study explores the toxic consequences of ethanol on human lung carcinoma cell, A549 in serum-deprived condition. Methodology: Human lung carcinoma cells, A549, were cultured in complete and serum-deprived medium for 6 hr. Subsequently, they were exposed to 50 mM and 100 mM concentrations of ethanol. Cytotoxicity studies linked with cell viability, oxidative stress, cell cycle arrest and micronuclei formation were performed using various toxicological parameters, namely MTT assay, DCFDA based ROS generation, cell cycle analysis and micronuclei formation assay. The cytotoxicity of ethanol in complete and serum deprived medium were compared at similar doses and time duration. Results: The metabolic viability assay demonstrated that 50 mM and 100 mM concentration of ethanol did not induce significant levels of cytotoxic alteration in A549 lung carcinoma cells in complete medium. However, in serum-deprived conditions, 50 mM and 100 mM ethanol concentration significantly altered cell viability. Further, exposure of 50 mM and 100 mM concentration of ethanol enhanced reactive oxygen species levels in A549 cells more significantly in serum-deprived conditions than in complete medium. In addition to cytotoxicity and oxidative stress, 50 mM and 100 mM ethanol also arrested the cells at G0 phase more significantly in serum deprived conditions compared to complete medium. Interpretation: Both 50 mM and 100 mM ethanol concentration enhanced the cell cytotoxicity and reactive oxygen species, cell cycle arrest and micronuclei formation more severely in serum-deprived medium than in complete medium (containing 10% FBS) under similar treatment conditions.

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