scholarly journals Optimizing flow cytometric DNA ploidy and S-phase fraction as independent prognostic markers for node-negative breast cancer specimens

Cytometry ◽  
2001 ◽  
Vol 46 (3) ◽  
pp. 121-135 ◽  
Author(s):  
C.B. Bagwell ◽  
G.M. Clark ◽  
F. Spyratos ◽  
A. Chassevent ◽  
P.-O. Bendahl ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Prognostic Markers ◽  
Dna Ploidy ◽  
S Phase ◽  
Phase Fraction ◽  
Node Negative ◽  
Flow Cytometric ◽  
Node Negative Breast Cancer

The value of S-phase and DNA ploidy analysis as prognostic markers for node-negative breast cancer in the australian setting

Pathology ◽  
1999 ◽  
Vol 31 (2) ◽  
pp. 90-94 ◽  
Author(s):  
Sue W.J. Wong ◽  
Anna M. Rangan ◽  
A. Michael Bilous ◽  
John Boyages ◽  
Val Gebski ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Prognostic Markers ◽  
Dna Ploidy ◽  
S Phase ◽  
Node Negative ◽  
Ploidy Analysis ◽  
Node Negative Breast Cancer

S-phase fraction after gating on epithelial cells predicts recurrence in node-negative breast cancer

1994 ◽  
Vol 59 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Sten Wingren ◽  
Olle Stå ◽  
Siw Sullivan ◽  
Ann Brisfors ◽  
Bo Nordenskjöld
Keyword(s):  
Breast Cancer ◽  
Epithelial Cells ◽  
S Phase ◽  
Phase Fraction ◽  
Node Negative ◽  
Node Negative Breast Cancer

Flow cytometric DNA ploidy defines patients with poor prognosis in node-negative breast cancer

2007 ◽  
Vol 56 (1) ◽  
pp. 16-25 ◽  
Author(s):  
Ingegerd Balslev ◽  
Ib J. Christensen ◽  
Birgitte Bruun Rasmussen ◽  
Jørgen K. Larsen ◽  
Anne E. Lykkesfeldt ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Poor Prognosis ◽  
Dna Ploidy ◽  
Node Negative ◽  
Flow Cytometric ◽  
Node Negative Breast Cancer ◽  
Patients With Poor Prognosis

DNA ploidy and the percentage of cells in S-phase as prognostic factors for women with lymph node negative breast cancer

Cancer ◽  
1994 ◽  
Vol 74 (6) ◽  
pp. 1752-1761 ◽  
Author(s):  
Thomas E. Witzig ◽  
James N. Ingle ◽  
Steven S. Cha ◽  
Daniel J. Schaid ◽  
Roxanne L. Tabery ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Prognostic Factors ◽  
Lymph Node ◽  
Dna Ploidy ◽  
S Phase ◽  
Node Negative ◽  
Node Negative Breast Cancer

Prognostic significance of S-phase fraction in good-risk, node-negative breast cancer patients.

1992 ◽  
Vol 10 (3) ◽  
pp. 428-432 ◽  
Author(s):  
G M Clark ◽  
M C Mathieu ◽  
M A Owens ◽  
L G Dressler ◽  
L Eudey ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Prognostic Significance ◽  
Axillary Node ◽  
S Phase ◽  
Phase Fraction ◽  
Breast Cancer Patients ◽  
Node Negative ◽  
Node Negative Breast Cancer ◽  
Disease Free

PURPOSE Formalin-fixed, paraffin-embedded tissues from axillary node-negative breast cancer patients were analyzed by flow cytometry to determine the prognostic significance of DNA ploidy and S-phase fraction (SPF). PATIENTS AND METHODS All patients were registered on a good-risk control arm of an intergroup clinical trial. They had small- to intermediate-sized (less than 3 cm), estrogen receptor (ER)-positive tumors and received no adjuvant therapy after modified radical mastectomy or total mastectomy with low axillary-node sampling. The median follow-up was 4.8 years. RESULTS Assessable ploidy results were obtained from 92% of the 298 specimens studied (51% diploid, 49% aneuploid), and SPFs were assessable for 83% of the tumors. SPFs for diploid tumors ranged from 0.7% to 11.9% (median, 3.6%), compared with a range of 1.2% to 26.7% (median, 7.6%) for aneuploid tumors (P less than .0001). No significant differences in disease-free or overall survival were observed between patients with diploid and aneuploid tumors. Using different SPF cutoffs by ploidy status (4.4% for diploid, 7.0% for aneuploid), patients with low SPFs had significantly longer disease-free survival rates than patients with high SPFs (P = .0008). The actuarial 5-year relapse rates were 15% and 32% for patients with low (n = 142) and high SPFs (n = 105), respectively. Similar relationships between SPF and clinical outcome were observed for patients with diploid tumors (P = .053) and for patients with aneuploid tumors (P = .0012). CONCLUSION S-phase fraction provides additional prognostic information for predicting disease-free survival for axillary node-negative breast cancer patients with small, ER-positive tumors.

scholarly journals Evaluation of Prognostic Factors Following Flow-Cytometric DNA Analysis after Cytokeratin Labelling: I. Breast Cancer

2002 ◽  
Vol 24 (4-5) ◽  
pp. 135-145 ◽  
Author(s):  
Pauline Wimberger ◽  
Peter Hillemanns ◽  
Thomas Kapsner ◽  
Hermann Hepp ◽  
Rainer Kimmig
Keyword(s):  
Breast Cancer ◽  
Prognostic Factors ◽  
Tumor Cells ◽  
Dna Ploidy ◽  
Gynecologic Oncology ◽  
Univariate Analysis ◽  
Prognostic Significance ◽  
S Phase ◽  
Phase Fraction ◽  
Flow Cytometric

In gynecologic oncology valid prognostic factors are necessary to estimate the course of disease and to define biologically similar subgroups for analysis of therapeutic efficacy. The presented study is a prospective study concerning prognostic significance of DNA ploidy and S‐phase fraction in breast cancer following enrichment of tumor cells by cytokeratin labelling. Epithelial cells were labeled by FITC‐conjugated cytokeratin antibody (CK 5, 6, 8, and CK 17) prior to flow cytometric cell cycle analysis in 327 fresh specimens of primary breast cancer. Univariate analysis in breast cancer detected the prognostic significance of DNA‐ploidy, S‐phase fraction and CV (coefficient of variation) of G0G1‐peak of tumor cells for clinical outcome, especially for nodal‐negative patients. Multivariate analysis could not confirm prognostic evidence of DNA‐ploidy and S‐phase fraction. In conclusion, in breast cancer no clinical significance for determination of DNA‐parameters was found.

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