scholarly journals Analysis of Ki-ras gene mutations associated with DNA diploid, aneuploid, and multiploid colorectal carcinomas using a crypt isolation technique

Cytometry ◽  
2001 ◽  
Vol 46 (6) ◽  
pp. 345-350 ◽  
Author(s):  
Tamotsu Sugai ◽  
Wataru Habano ◽  
Shin-ichi Nakamura ◽  
Yu-Fei Jiao ◽  
Taro Higuchi ◽  
...  
Keyword(s):  
Gene Mutations ◽  
Colorectal Carcinomas ◽  
Ras Gene ◽  
Isolation Technique ◽  
Crypt Isolation

scholarly journals Genetic alterations in DNA diploid, aneuploid and multiploid colorectal carcinomas identified by the crypt isolation technique

2000 ◽  
Vol 88 (4) ◽  
pp. 614-619 ◽  
Author(s):  
Tamotsu Sugai ◽  
Wataru Habano ◽  
Shin-ichi Nakamura ◽  
Hajime Sato ◽  
Noriyuki Uesugi ◽  
...  
Keyword(s):  
Genetic Alterations ◽  
Colorectal Carcinomas ◽  
Isolation Technique ◽  
Crypt Isolation

A Unique Method for Mutation Analysis of Tumor Suppressor Genes in Colorectal Carcinomas Using a Crypt Isolation Technique

2000 ◽  
Vol 124 (3) ◽  
pp. 382-386 ◽  
Author(s):  
Tamotsu Sugai ◽  
Wataru Habano ◽  
Shin-ichi Nakamura ◽  
Noriyuki Uesugi ◽  
Shunichi Sasou ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Genetic Analysis ◽  
Tumor Suppressor Genes ◽  
Single Strand Conformation Polymorphism ◽  
Single Strand ◽  
Colorectal Carcinomas ◽  
Suppressor Genes ◽  
Isolation Technique ◽  
Crypt Isolation ◽  
Conformation Polymorphism

Abstract Background.—Contamination of nontumor tissue makes genetic analysis difficult. For this reason, it is important to obtain pure tumor tissue to ensure accurate genetic analysis. Objective.—To accurately assess the incidence of mutation of tumor suppressor genes (p53: exon 5–8; APC: mutated cluster region; NF-2 gene: all exons) in 45 colorectal carcinomas. Methods.—We developed an application of the polymerase chain reaction–single-strand conformation polymorphism and DNA sequence by coupling them with crypt isolation. Results.—Mutations of p53 and APC genes were found in 24 and 22 of 45 colorectal carcinomas, respectively. No mutation of the NF-2 gene was observed in this cancer. Single-strand conformation polymorphism using a crypt isolation technique showed a clear migrating band and no false-positive data. Conclusions.—The crypt isolation technique is a useful method for accurately analyzing genetic alterations. Furthermore, our proposed method confirmed the morphological findings obtained before the genetic analysis.

Application of the crypt isolation technique to the assessment of genetic alterations of colorectal carcinomas

2002 ◽  
Vol 52 (10) ◽  
pp. 628-635 ◽  
Author(s):  
Hiroshi Takahashi ◽  
Tamotsu Sugai ◽  
Wataru Habano ◽  
Shin-ichi Nakamura
Keyword(s):  
Genetic Alterations ◽  
Colorectal Carcinomas ◽  
Isolation Technique ◽  
Crypt Isolation

Analysis of Ki-ras gene mutations within the same tumor using a single tumor crypt in colorectal carcinomas

2004 ◽  
Vol 39 (6) ◽  
pp. 544-549 ◽  
Author(s):  
Motohiro Ishii ◽  
Tamotsu Sugai ◽  
Wataru Habano ◽  
Shin-ichi Nakamura
Keyword(s):  
Gene Mutations ◽  
Colorectal Carcinomas ◽  
Ras Gene ◽  
Single Tumor

PTU-079 K-ras gene mutations in pancreatic cancer and chronic pancreatitis patients in North Indian population

Gut ◽  
2010 ◽  
Vol 59 (Suppl 1) ◽  
pp. A81.1-A81
Author(s):  
S K Polipalli ◽  
P Kar ◽  
S A Husain ◽  
A Agarwal ◽  
R Gondal
Keyword(s):  
Pancreatic Cancer ◽  
Chronic Pancreatitis ◽  
Indian Population ◽  
Gene Mutations ◽  
North Indian Population ◽  
Ras Gene

Differential Expression of ras Signal Transduction Mediators in Verrucous and Squamous Cell Carcinomas of the Upper Aerodigestive Tract

2001 ◽  
Vol 125 (9) ◽  
pp. 1200-1203
Author(s):  
Julie L. Lessard ◽  
Robert A. Robinson ◽  
Henry T. Hoffman
Keyword(s):  
Signal Transduction ◽  
Squamous Cell ◽  
Gene Mutations ◽  
Squamous Cell Carcinomas ◽  
Aerodigestive Tract ◽  
Upper Aerodigestive Tract ◽  
Immunohistochemical Expression ◽  
Ras Gene ◽  
Extracellular Signal ◽  
Ras Signal Transduction

Abstract Context.—ras gene mutations and expression of its gene product have been described in verrucous and squamous cell carcinomas. Other downstream signal-transduction mediators, extracellular signal-regulated kinases 1 and 2 (ERK-1 and ERK-2) and Raf-1, have not yet been as extensively studied. Objective.—To determine patterns of expression of ERK-1, ERK-2, and Raf-1 in verrucous and squamous cell carcinomas of the upper aerodigestive tract. Design.—Seventeen verrucous carcinomas and 10 squamous cell carcinomas of the upper aerodigestive tract were examined for the immunohistochemical expression of ERK-1, ERK-2, and Raf-1 product. Results.—Raf-1 expression was intensely expressed in the most basal portions of the epithelium in verrucous carcinomas, but was minimally expressed in the suprabasalar areas. Anti-Raf-1 staining of the squamous cell carcinomas was diffuse and patchy throughout the tumor cells and was weak in intensity. There was no geographic preference of staining. The cytoplasmic expression of both ERK-1 and ERK-2 was predominantly negative in the most basal layers of the epithelium in the verrucous carcinomas, but was positive in the suprabasalar region of the epithelium. Immunohistochemical expression of ERK-1 and ERK-2 in the squamous carcinomas was diffuse throughout the tumor. Conclusion.—There is strong correlation of the geographic expression of these mediators of ras signal transduction in verrucous and squamous carcinomas, but the cause of these differences remains unclear at present. The expression of these mediator proteins may have potential for diagnosis, as well as in understanding the biologic behavior of these lesions.

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