scholarly journals Diversity and intratumoral heterogeneity in human gallbladder cancer progression revealed by single‐cell RNA sequencing

2021 ◽  
Vol 11 (6) ◽  
Author(s):  
Peizhan Chen ◽  
Yueqi Wang ◽  
Jingquan Li ◽  
Xiaobo Bo ◽  
Jie Wang ◽  
...  
Keyword(s):  
Gallbladder Cancer ◽  
Single Cell ◽  
Rna Sequencing ◽  
Cancer Progression ◽  
Intratumoral Heterogeneity ◽  
Human Gallbladder ◽  
Single Cell Rna Sequencing

scholarly journals LGG-05. SINGLE-CELL RNA SEQUENCING OF PEDIATRIC LOW-GRADE GLIOMAS REVEALS INTRATUMORAL HETEROGENEITY

2017 ◽  
Vol 19 (suppl_4) ◽  
pp. iv33-iv34
Author(s):  
Brenton Paolella ◽  
Pratiti Bandopadhayay ◽  
Guillaume Bergthold ◽  
Alex Shalek ◽  
Kristine Pelton ◽  
...  
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Intratumoral Heterogeneity ◽  
Low Grade ◽  
Low Grade Gliomas ◽  
Single Cell Rna Sequencing

scholarly journals Identification of EMT signaling cross-talk and gene regulatory networks by single-cell RNA sequencing

2021 ◽  
Vol 118 (19) ◽  
pp. e2102050118
Author(s):  
Abhijeet P. Deshmukh ◽  
Suhas V. Vasaikar ◽  
Katarzyna Tomczak ◽  
Shubham Tripathi ◽  
Petra den Hollander ◽  
...  
Keyword(s):  
Single Cell ◽  
Signaling Pathways ◽  
Rna Sequencing ◽  
Cancer Progression ◽  
Tyrosine Kinases ◽  
Regulatory Networks ◽  
Expression Profiles ◽  
Critical Role ◽  
Mesenchymal Transition ◽  
Single Cell Rna Sequencing

The epithelial-to-mesenchymal transition (EMT) plays a critical role during normal development and in cancer progression. EMT is induced by various signaling pathways, including TGF-β, BMP, Wnt–β-catenin, NOTCH, Shh, and receptor tyrosine kinases. In this study, we performed single-cell RNA sequencing on MCF10A cells undergoing EMT by TGF-β1 stimulation. Our comprehensive analysis revealed that cells progress through EMT at different paces. Using pseudotime clustering reconstruction of gene-expression profiles during EMT, we found sequential and parallel activation of EMT signaling pathways. We also observed various transitional cellular states during EMT. We identified regulatory signaling nodes that drive EMT with the expression of important microRNAs and transcription factors. Using a random circuit perturbation methodology, we demonstrate that the NOTCH signaling pathway acts as a key driver of TGF-β–induced EMT. Furthermore, we demonstrate that the gene signatures of pseudotime clusters corresponding to the intermediate hybrid EMT state are associated with poor patient outcome. Overall, this study provides insight into context-specific drivers of cancer progression and highlights the complexities of the EMT process.

scholarly journals 182 Single-cell RNA-sequencing reveals SCC intratumoral heterogeneity

2018 ◽  
Vol 138 (5) ◽  
pp. S31
Author(s):  
A. Ji ◽  
A. Rubin ◽  
S. Hollmig ◽  
S. Aasi ◽  
P. Khavari
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Intratumoral Heterogeneity ◽  
Single Cell Rna Sequencing

41-OR: Single Cell RNA-Sequencing Reveals the Developmental Heterogeneity of Brown Adipocytes

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 41-OR
Author(s):  
FARNAZ SHAMSI ◽  
MARY PIPER ◽  
LI-LUN HO ◽  
TIAN LIAN HUANG ◽  
YU-HUA TSENG
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Brown Adipocytes ◽  
Single Cell Rna Sequencing
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