Gene pyramiding combinations to confers resistance of Asian soybean rust

Crop Science ◽  
2021 ◽  
Author(s):  
Daniela Meira ◽  
Maiara Cecília Panho ◽  
Eduardo Beche ◽  
Leomar Guilherme Woyann ◽  
Laura Alexandra Madella ◽  
...  
Keyword(s):  
Gene Pyramiding ◽  
Soybean Rust ◽  
Asian Soybean Rust

scholarly journals Identification of a Second Asian Soybean Rust Resistance Gene in Hyuuga Soybean

2011 ◽  
Vol 101 (5) ◽  
pp. 535-543 ◽  
Author(s):  
Mandy D. Kendrick ◽  
Donna K. Harris ◽  
Bo-Keun Ha ◽  
David L. Hyten ◽  
Perry B. Cregan ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Recombinant Inbred Lines ◽  
Gene Pyramiding ◽  
Soybean Rust ◽  
Phakopsora Pachyrhizi ◽  
Natural Case ◽  
Asian Soybean Rust ◽  
Resistance Gene Mapping ◽  
Significant Disease ◽  
Phenotypic Responses

Asian soybean rust (ASR) is an economically significant disease caused by the fungus Phakopsora pachyrhizi. The soybean genes Rpp3 and Rpp?(Hyuuga) confer resistance to specific isolates of the pathogen. Both genes map to chromosome 6 (Gm06) (linkage group [LG] C2). We recently identified 12 additional soybean accessions that harbor ASR resistance mapping to Gm06, within 5 centimorgans of Rpp3 and Rpp?(Hyuuga). To further characterize genotypes with resistance on Gm06, we used a set of eight P. pachyrhizi isolates collected from geographically diverse areas to inoculate plants and evaluate them for differential phenotypic responses. Three isolates elicited different responses from soybean accessions PI 462312 (Ankur) (Rpp3) and PI 506764 (Hyuuga) (Rpp?[Hyuuga]). In all, 11 of the new accessions yielded responses identical to either PI 462312 or Hyuuga and 1 of the new accessions, PI 417089B (Kuro daizu), differed from all others. Additional screening of Hyuuga-derived recombinant inbred lines indicated that Hyuuga carries two resistance genes, one at the Rpp3 locus on Gm06 and a second, unlinked ASR resistance gene mapping to Gm03 (LG-N) near Rpp5. These findings reveal a natural case of gene pyramiding for ASR resistance in Hyuuga and underscore the importance of utilizing multiple isolates of P. pachyrhizi when screening for ASR resistance.

scholarly journals Microsatellite molecular marker-assisted gene pyramiding for resistance to Asian soybean rust (ASR)

2018 ◽  
Vol 40 (1) ◽  
pp. 39619
Author(s):  
Joselaine Viganó ◽  
Alessandro Lucca Braccini ◽  
Ivan Schuster ◽  
Vanessa Maria Pereira Silva Menezes
Keyword(s):  
Molecular Marker ◽  
Gene Pyramiding ◽  
Soybean Rust ◽  
Asian Soybean Rust

Multiple Rpp-gene pyramiding confers resistance to Asian soybean rust isolates that are virulent on each of the pyramided genes

2015 ◽  
Vol 40 (5) ◽  
pp. 283-290 ◽  
Author(s):  
Naoki Yamanaka ◽  
Mio Morishita ◽  
Tomomi Mori ◽  
Noelle G. Lemos ◽  
Md. M. Hossain ◽  
...  
Keyword(s):  
Gene Pyramiding ◽  
Soybean Rust ◽  
Asian Soybean Rust

Asian Soybean Rust Sentinel Plots

2006 ◽  
Author(s):  
Ralph von Qualen ◽  
Xiao-Bing Yang
Keyword(s):  
Soybean Rust ◽  
Asian Soybean Rust

Asian Soybean Rust Sentinel Plots

2006 ◽  
Author(s):  
Ralph von Qualen ◽  
Xiao-Bing Yang
Keyword(s):  
Soybean Rust ◽  
Asian Soybean Rust

Identification and classification of Asian soybean rust using leaf-based hyperspectral reflectance

2021 ◽  
Vol 42 (11) ◽  
pp. 4177-4198
Author(s):  
Renato Herrig Furlanetto ◽  
Marcos Rafael Nanni ◽  
Monica Sayuri Mizuno ◽  
Luís Guilherme Teixeira Crusiol ◽  
Camila Rocco da Silva
Keyword(s):  
Soybean Rust ◽  
Hyperspectral Reflectance ◽  
Asian Soybean Rust

Registration of Asian Soybean Rust-Resistant Soybean Germplasm G01-PR16

2011 ◽  
Vol 5 (1) ◽  
pp. 118-122 ◽  
Author(s):  
H. Roger Boerma ◽  
Maria J. Monteros ◽  
Bo-Keun Ha ◽  
E. Dale Wood ◽  
Daniel V. Phillips ◽  
...  
Keyword(s):  
Soybean Rust ◽  
Soybean Germplasm ◽  
Asian Soybean Rust

scholarly journals Transcriptome analyses and virus induced gene silencing identify genes in the Rpp4-mediated Asian soybean rust resistance pathway

2013 ◽  
Vol 40 (10) ◽  
pp. 1029 ◽  
Author(s):  
Aguida M. A. P. Morales ◽  
Jamie A. O'Rourke ◽  
Martijn van de Mortel ◽  
Katherine T. Scheider ◽  
Timothy J. Bancroft ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Gene Silencing ◽  
Differentially Expressed ◽  
Soybean Rust ◽  
Phakopsora Pachyrhizi ◽  
R Gene ◽  
Virus Induced Gene Silencing ◽  
Asian Soybean Rust ◽  
Defence Responses

Rpp4 (Resistance to Phakopsora pachyrhizi 4) confers resistance to Phakopsora pachyrhizi Sydow, the causal agent of Asian soybean rust (ASR). By combining expression profiling and virus induced gene silencing (VIGS), we are developing a genetic framework for Rpp4-mediated resistance. We measured gene expression in mock-inoculated and P. pachyrhizi-infected leaves of resistant soybean accession PI459025B (Rpp4) and the susceptible cultivar (Williams 82) across a 12-day time course. Unexpectedly, two biphasic responses were identified. In the incompatible reaction, genes induced at 12 h after infection (hai) were not differentially expressed at 24 hai, but were induced at 72 hai. In contrast, genes repressed at 12 hai were not differentially expressed from 24 to 144 hai, but were repressed 216 hai and later. To differentiate between basal and resistance-gene (R-gene) mediated defence responses, we compared gene expression in Rpp4-silenced and empty vector-treated PI459025B plants 14 days after infection (dai) with P. pachyrhizi. This identified genes, including transcription factors, whose differential expression is dependent upon Rpp4. To identify differentially expressed genes conserved across multiple P. pachyrhizi resistance pathways, Rpp4 expression datasets were compared with microarray data previously generated for Rpp2 and Rpp3-mediated defence responses. Fourteen transcription factors common to all resistant and susceptible responses were identified, as well as fourteen transcription factors unique to R-gene-mediated resistance responses. These genes are targets for future P. pachyrhizi resistance research.

scholarly journals Distinct Biphasic mRNA Changes in Response to Asian Soybean Rust Infection

2007 ◽  
Vol 20 (8) ◽  
pp. 887-899 ◽  
Author(s):  
Martijn van de Mortel ◽  
Justin C. Recknor ◽  
Michelle A. Graham ◽  
Dan Nettleton ◽  
Jaime D. Dittman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Time Course ◽  
Expression Profiles ◽  
Fungal Growth ◽  
Initial Response ◽  
Soybean Rust ◽  
Specific Response ◽  
Susceptible Genotype ◽  
Rust Infection ◽  
Asian Soybean Rust

Asian soybean rust (ASR), caused by Phakopsora pachyrhizi, is now established in all major soybean-producing countries. Currently, there is little information about the molecular basis of ASR–soybean interactions, which will be needed to assist future efforts to develop effective resistance. Toward this end, abundance changes of soybean mRNAs were measured over a 7-day ASR infection time course in mock-inoculated and infected leaves of a soybean accession (PI230970) carrying the Rpp2 resistance gene and a susceptible genotype (Embrapa-48). The expression profiles of differentially expressed genes (ASR-infected compared with the mock-inoculated control) revealed a biphasic response to ASR in each genotype. Within the first 12 h after inoculation (hai), which corresponds to fungal germination and penetration of the epidermal cells, differential gene expression changes were evident in both genotypes. mRNA expression of these genes mostly returned to levels found in mock-inoculated plants by 24 hai. In the susceptible genotype, gene expression remained unaffected by rust infection until 96 hai, a time period when rapid fungal growth began. In contrast, gene expression in the resistant genotype diverged from the mock-inoculated control earlier, at 72 h, demonstrating that Rpp2-mediated defenses were initiated prior to this time. These data suggest that ASR initially induces a non-specific response that is transient or is suppressed when early steps in colonization are completed in both soybean genotypes. The race-specific resistance phenotype of Rpp2 is manifested in massive gene expression changes after the initial response prior to the onset of rapid fungal growth that occurs in the susceptible genotype.

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