Differential expression of gibberellin‐related genes in wild type and dwarf mutant of Poa pratensis implied their roles in regulating plant height

Crop Science ◽  
2021 ◽  
Author(s):  
Lu Gan ◽  
Haotian Su ◽  
Mengju Yan ◽  
Liebao Han ◽  
Shuxia Yin
Keyword(s):  
Differential Expression ◽  
Plant Height ◽  
Poa Pratensis ◽  
Dwarf Mutant ◽  
Wild Type

scholarly journals Characterization and Fine Mapping of a New Dwarf Mutant in Brassica Napus

2020 ◽  
Author(s):  
Xin Li ◽  
Fujiang Xiang ◽  
Wei Zhang ◽  
Jindong Yan ◽  
Xinmei Li ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Grain Yield ◽  
Plant Height ◽  
Fine Mapping ◽  
Plant Architecture ◽  
Hybrid Breeding ◽  
Dwarf Mutant ◽  
Wild Type ◽  
Lodging Resistance ◽  
Commercial Cultivar

Abstract Background: Plant height is an important plant architecture character closely related to yield performance of many crops. Reasonable reduction of plant height of crops is beneficial for enhancing lodging resistance and improving yield. Results: In the present study, we described a Brassica napus dwarf mutant bnd2 induced by ethyl methanesulfonate (EMS) mutagenesis. Compared to wild type, bnd2 showed shorter stature, shorter hypocotyl, as well as shorter petiole leaves. We crossed the bnd2 mutant with its wild type and found that the ratio of the mutant to the wild type in the F2 population was close to 1:3, indicating that bnd2 is a recessive mutation of a single locus. Following bulked segregant analysis (BSA) by resequencing, BND2 was located into the 13.77 Mb-18.08 Mb interval of chromosome A08, with a length of 4.31 Mb. After fine mapping with SNP and InDel markers, the gene was narrowed to a 140-Kb interval ranging from 15.62 Mb to 15.76 Mb. According to reference genome annotation, there are 27 genes in the interval, and one of them BnaA08g20960D has a SNP type variation in the intron between the mutant and its parent, which may be the candidate gene conferring to BND2. The hybrid line derived from a cross between the mutant bnd2 and a commercial cultivar L329 has similar plant height but higher grain yield than the commercial cultivar, suggesting that the allele bnd2 is benefit for hybrid breeding of lodging resistance and high yield in rapeseed.Conclusion: In this study, we found a fresh resource and a new locus for dwarf in rapeseed, which may be benefit for functional analysis of genetic mechanism of plant architecture and grain yield in rapeseed.

Effects of an Autoregulatory Senescence-inhibitor Gene Construct on Nicotiana alata Link and Otto.

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 519d-519 ◽  
Author(s):  
Kenneth R. Schroeder ◽  
Dennis P. Stimart
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Plant Height ◽  
Dry Weight ◽  
Nicotiana Alata ◽  
Wild Type ◽  
Gene Encoding ◽  
Delayed Senescence ◽  
Gene Construct ◽  
Shoot Dry Weight ◽  
Inhibitor System

Nicotiana alata Link and Otto. was transformed via Agrobacterium tumefaciens encoding a senescence-specific promoter SAG12 cloned from Arabidopsis thaliana fused to a Agrobacterium tumefaciens gene encoding isopentenyl transferase (IPT) that catalyzes cytokinin synthesis. This was considered an autoregulatory senescence-inhibitor system. In 1996, we reported delayed senescence of intact flowers by 2 to 6 d and delayed leaf senescence of transgenic vs. wild-type N. alata. Further evaluations in 1997 revealed several other interesting effects of the SAG12-IPT gene construct. Measurement of chlorophyll content of mature leaves showed higher levels of both chlorophyll a and b in transgenic material under normal fertilization and truncated fertilization regimes. At 4 to 5 months of age transgenic plants expressed differences in plant height, branching, and dry weight. Plant height was reduced by 3 to 13 cm; branch counts increased 2 to 3 fold; and shoot dry weight increased up to 11 g over wild-type N. alata. These observations indicate the system is not tightly autoregulated and may prove useful to the floriculture industry for producing compact and more floriferous plants.

scholarly journals Differential expression of transporters for norepinephrine and glutamate in wild type, variant, and WNT1-expressing PC12 cells.

1993 ◽  
Vol 268 (32) ◽  
pp. 23891-23897
Author(s):  
B Ramachandran ◽  
K Houben ◽  
Y.Y. Rozenberg ◽  
J.R. Haigh ◽  
A Varpetian ◽  
...  
Keyword(s):  
Differential Expression ◽  
Pc12 Cells ◽  
Wild Type

scholarly journals Altered Promoter and G-Box Binding Factor for 1-Deoxy-d-Xylulose-5-Phosphate Synthase Gene Grown from Poa pratensis Seeds after Spaceflight

2019 ◽  
Vol 20 (6) ◽  
pp. 1398
Author(s):  
Lu Gan ◽  
Yuehui Chao ◽  
Haotian Su ◽  
Yujing Ren ◽  
Shuxia Yin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Promoter Region ◽  
Poa Pratensis ◽  
Regulatory Region ◽  
Space Environment ◽  
Luciferase Assay ◽  
Dwarf Mutant ◽  
Transcript Accumulation ◽  
Binding Factor ◽  
Phosphate Synthase

In plant cells, the nucleus DNA is considered the primary site of injury by the space environment, which could generate genetic alteration. As the part of genomic mutation, genetic variation in the promoter region could regulate gene expression. In the study, it is observed that there is a deletion in the upstream regulatory region of the 1-deoxy-d-xylulose-5-phosphate synthase 1 gene (PpDXS1) of Poa pratensis dwarf mutant and the PpDXS1 transcript abundance is lower in the dwarf mutant. It is indicated that the deletion in the promoter region between wild type and dwarf mutant could be responsible for the regulation of PpDXS1 gene expression. The PpDXS1 promoter of dwarf mutant shows a lower activity as determined by dual luciferase assay in Poa pratensis protoplast, as well as the GUS activity is lower in transgenic Poa pratensis plant. To further investigate the effect of the deletion in the promoter region on PpDXS1 transcript accumulation, the transient assay and yeast one-hybrid experiment demonstrate that the deletion comprises a motif which is a target of G-box binding factor (GBF1), and the motif correlates with an increase in transactivation by GBF1 protein. Taken together, these results indicate that the deletion in the promoter of PpDXS1 isolated from dwarf mutant is sufficient to account for the decrease in PpDXS1 transcript level and GBF1 can regulate the PpDXS1 gene expression, and subsequently affect accumulation of various isoprenoids throughout the plant.

scholarly journals Identification of Bna.IAA7.C05 as allelic gene for dwarf mutant generated from tissue culture in oilseed rape

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Hongtao Cheng ◽  
Fenwei Jin ◽  
Qamar U. Zaman ◽  
Bingli Ding ◽  
Mengyu Hao ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Height ◽  
High Throughput Sequencing ◽  
Agronomic Traits ◽  
Crop Improvement ◽  
Dwarf Mutant ◽  
Sequencing Analysis ◽  
Rna Seq ◽  
Dwarf Phenotype ◽  
Lodging Resistance

Abstract Background Plant height is one of the most important agronomic traits in many crops due to its influence on lodging resistance and yield performance. Although progress has been made in the use of dwarfing genes in crop improvement, identification of new dwarf germplasm is still of significant interest for breeding varieties with increased yield. Results Here we describe a dominant, dwarf mutant G7 of Brassica napus with down-curved leaves derived from tissue culture. To explore the genetic variation responsible for the dwarf phenotype, the mutant was crossed to a conventional line to develop a segregating F2 population. Bulks were formed from plants with either dwarf or conventional plant height and subjected to high throughput sequencing analysis via mutation mapping (MutMap). The dwarf mutation was mapped to a 0.6 Mb interval of B. napus chromosome C05. Candidate gene analysis revealed that one SNP causing an amino acid change in the domain II of Bna.IAA7.C05 may contribute to the dwarf phenotype. This is consistent with the phenotype of a gain-of-function indole-3-acetic acid (iaa) mutant in Bna.IAA7.C05 reported recently. GO and KEGG analysis of RNA-seq data revealed the down-regulation of auxin related genes, including many other IAA and small up regulated response (SAUR) genes, in the dwarf mutant. Conclusion Our studies characterize a new allele of Bna.IAA7.C05 responsible for the dwarf mutant generated from tissue culture. This may provide a valuable genetic resource for breeding for lodging resistance and compact plant stature in B. napus.

Differential expression of tyrosine hydroxylase in catecholaminergic neurons of neonatal wild-type and nurr1-deficient mice

Neuroscience ◽  
1999 ◽  
Vol 93 (2) ◽  
pp. 631-642 ◽  
Author(s):  
J.S. Baffi ◽  
M. Palkovits ◽  
S.O. Castillo ◽  
È. Mezey ◽  
V.M. Nikodem
Keyword(s):  
Tyrosine Hydroxylase ◽  
Differential Expression ◽  
Wild Type ◽  
Catecholaminergic Neurons ◽  
Deficient Mice

Differential expression of prohibitin and regulation of apoptosis in wild-type and COX-2 null mouse embryonic fibroblasts

2009 ◽  
Vol 53 (2) ◽  
pp. 157-159 ◽  
Author(s):  
Young-Hoon Kim ◽  
Hyangkyu Lee ◽  
Tae-Yoon Kim ◽  
Hyang-Ran Hwang ◽  
Sang Chul Lee
Keyword(s):  
Differential Expression ◽  
Null Mouse ◽  
Wild Type ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Cox 2

scholarly journals Mapping of dwarfing QTL of Ari1327, a semi-dwarf mutant of upland cotton

2022 ◽  
Vol 22 (1) ◽  
Author(s):  
Chenhui Ma ◽  
Abdul Rehman ◽  
Hong Ge Li ◽  
Zi Bo Zhao ◽  
Gaofei Sun ◽  
...  
Keyword(s):  
Plant Height ◽  
Upland Cotton ◽  
Genotyping By Sequencing ◽  
Dwarf Mutant ◽  
Rna Seq ◽  
Indel Markers ◽  
Linkage Information ◽  
Wild Variety ◽  
Key Features ◽  
Genotype A

Abstract Background Upland Cotton (Gossypium hirsutum L.) has few cotton varieties suitable for mechanical harvesting. The plant height of the cultivar is one of the key features that need to modify. Hence, this study was planned to locate the QTL for plant height in a 60Co γ treated upland cotton semi-dwarf mutant Ari1327. Results Interestingly, bulk segregant analysis (BSA) and genotyping by sequencing (GBS) methods exhibited that candidate QTL was co-located in the region of 5.80–9.66 Mb at D01 chromosome in two F2 populations. Using three InDel markers to genotype a population of 1241 individuals confirmed that the offspring’s phenotype is consistent with the genotype. Comparative analysis of RNA-seq between the mutant and wild variety exhibited that Gh_D01G0592 was identified as the source of dwarfness from 200 genes. In addition, it was also revealed that the appropriate use of partial separation markers in QTL mapping can escalate linkage information. Conclusions Overwhelmingly, the results will provide the basis to reveal the function of candidate genes and the utilization of excellent dwarf genetic resources in the future.

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