scholarly journals DNA content and ploidy estimation of Festuca ovina accessions by flow cytometry

Crop Science ◽  
2020 ◽  
Vol 60 (5) ◽  
pp. 2757-2767 ◽  
Author(s):  
Yinjie Qiu ◽  
Sierra Hamernick ◽  
Joan Barreto Ortiz ◽  
Eric Watkins
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Festuca Ovina

scholarly journals DNA Content and Ploidy Estimation of Festuca ovina Accessions by Flow Cytometry

2020 ◽  
Author(s):  
Yinjie Qiu ◽  
Sierra Hamernick ◽  
Joan Barreto Ortiz ◽  
Eric Watkins
Keyword(s):  
Flow Cytometry ◽  
Seed Size ◽  
Dna Content ◽  
Breeding Programs ◽  
Festuca Ovina ◽  
Ploidy Levels ◽  
Plant Germplasm ◽  
Wide Range ◽  
National Plant Germplasm System ◽  
Fine Fescue

ABSTRACTFestuca ovina is a fine fescue that is used as a low-input turfgrass. The ploidy levels of F. ovina accessions held by the USDA National Plant Germplasm System (NPGS) are unknown, limiting the use of the germplasm in breeding programs. The objective of this study was to determine DNA content and estimate ploidy of these 127 accessions. Among the accessions, we identified a wide range of ploidy levels from diploid to octoploid. We also found the accessions with higher ploidy levels usually had larger seed size. These results will be informative to plant breeders and researchers using germplasm from the F. ovina collection and point to challenges in maintaining polyploid, outcrossing germplasm seed stocks in common nurseries.

Estimation of nuclear DNA content by flow cytometry in fishes of the genus Xiphophorus

1989 ◽  
Vol 94 (3) ◽  
pp. 465-468 ◽  
Author(s):  
Terrence R. Tiersch ◽  
Robert W. Chandler ◽  
Klaus D. Kallman ◽  
Stephen S. Wachtel
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content

scholarly journals Brief communication. First evaluation of nuclear DNA content in Setaria genus by flow cytometry

1998 ◽  
Vol 89 (6) ◽  
pp. 556-559 ◽  
Author(s):  
M Le Thierry d'Ennequin ◽  
O Panaud ◽  
S Brown ◽  
S Siljak-Yakovlev ◽  
A Sarr
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content

scholarly journals Touch Imprint Intraoperative Flow Cytometry as a Complementary Tool for Detailed Assessment of Resection Margins and Tumor Biology in Liver Surgery for Primary and Metastatic Liver Neoplasms

2021 ◽  
Vol 4 (3) ◽  
pp. 66
Author(s):  
Georgios S. Markopoulos ◽  
Georgios K. Glantzounis ◽  
Anna C. Goussia ◽  
Georgios D. Lianos ◽  
Anastasia Karampa ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Liver Tumors ◽  
Tumor Biology ◽  
Surgical Margins ◽  
Distribution Analysis ◽  
Cell Analysis ◽  
Term Survival ◽  
Detailed Assessment ◽  
Metastatic Liver

Liver resection is the main treatment for primary and metastatic liver tumors in order to achieve long-term survival with good quality of life. The ultimate goal of surgical oncology is to achieve complete tumor removal with adequate clear surgical margins. Flow cytometry is a powerful analytical technique with applications such as phenotypic analysis and quantification of DNA content. Intraoperative flow cytometry (iFC) is the application of flow cytometry for DNA content/ploidy and cell cycle distribution analysis during surgery for tumor cell analysis and margin evaluation. It has been used for cell analysis of intracranial tumors and recently of head and neck carcinomas and breast carcinomas, as well as for tumor margin evaluation. Herein, we present a novel touch imprint iFC protocol for the detailed assessment of tumor margins during excision of malignant hepatic lesions. The protocol aims to offer information on surgical margins after removal of malignant liver tumors based on DNA content of cancer cells and to corroborate the results of iFC with that of histopathological analysis. Based on the established role of iFC in other types of malignancies, our specialized protocol has the potential, through characterization of cells in liver transection surface post hepatectomy, to offer significant information on the type of resection and tumor biology. This information can be used to effectively guide intra- and postoperative patient management.

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