An Efficient Method for DNA Purification‐Free PCR from Plant Tissue

2021 ◽  
Vol 1 (11) ◽  
Author(s):  
Zhengnan Jia ◽  
Xiaowei Han ◽  
Kenichi Tsuda
Keyword(s):  
Efficient Method ◽  
Plant Tissue ◽  
Dna Purification

A Simple and Efficient Method for DNA Purification from Samples of Highly Clotted Blood

2010 ◽  
Vol 46 (3) ◽  
pp. 258-264 ◽  
Author(s):  
Ruyi Xu ◽  
Ping Ye ◽  
Leiming Luo ◽  
Hongmei Wu ◽  
Jin Dong ◽  
...  
Keyword(s):  
Efficient Method ◽  
Dna Purification

scholarly journals Pengembangan Protokol Isolasi DNA Genom Tanaman Durian Dengan Menggunakan Modifikasi Bufer CTAB

2018 ◽  
Vol 6 (02) ◽  
pp. 33
Author(s):  
Sundari Sundari
Keyword(s):  
Efficient Method ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Dna Purification ◽  
Fruit Crops ◽  
Purification Step ◽  
High Quality ◽  
Plant Dna ◽  
Genomic Dna Isolation

AbstrakProtokol dan metode sederhana, efisien untuk isolasi  DNA genom tanaman durian   yang banyak mengandung phenol dan residu polisakarida telah dihasilkan. Pada penelitian ini, digunakan  protokol isolasi DNA tumbuhan  dengan metode CTAB yang dimodifikasi sebagai protokol yang  efisien untuk membuang polisakarida, phenol  dan  lendir yang sangat melimpah pada tanaman durian. Obyek penelitian ini  terdiri dari protocol CTAB yang dimodifikasi  tahap inkubasi dan presipitasi  pemurnian DNA genom dari phenol dan polisakarida. Perbandingan 2  protokol  isolasi  DNA durian dengan CTAB standard an CTAB modifikasi  menunjukkan bahwa metode  CTAB modifikasi menghasilkan  whole genom durian cukup murni rata rata 1,99 dan berhasil diamplifikasi  dengan PCR-RAPD.Kata kunci:  isolasi, DNA, polisakarida, CTAB, modifikasi, . AbstractThe simple and efficient  method for genomic DNA isolation protochol from durian , its woody fruit crops containing high polysaccharide levels has been described here. In the present study, using modified CTAB for plant DNA isolation protocols were studied for removing the highly concentrated polysaccharides from genomic DNA of woody fruit crops.This method involves the modified CTAB   at the incubate  and precipitate procedure employing DNA purification  step to remove polysaccharides and phenol residu.  Compared with the two  studied DNA  isolation protocols of  durian using standart CTAB and modified CTAB  the everage yield  high quality DNA whole genom is 1,99 purity and DNA was suitable for PCR and RAPD analyses.Keyword: isolation, DNA,  polysaccharides,  phenol residu, CTAB

DNA Purification-free PCR from Plant Tissue

2021 ◽  
Author(s):  
Zhengnan Jia ◽  
Miaomiao Ding ◽  
Masahito Nakano ◽  
Kunqi Hong ◽  
Ruidong Huang ◽  
...  
Keyword(s):  
Plant Tissue ◽  
Dna Purification

Freeze-fracture, freeze-etch complementary pairs of virus-infected cells reveal value of etching even when relatively high concentrations of cryoprotectants are used

1978 ◽  
Vol 36 (2) ◽  
pp. 136-137
Author(s):  
Russell L. Steere ◽  
Eric F. Erbe
Keyword(s):  
Plant Tissue ◽  
Phosphate Buffer ◽  
Infected Plant ◽  
Freeze Fracture ◽  
Distilled Water ◽  
Virus Infected Plant ◽  
Infected Cells ◽  
High Concentrations

It has been assumed by many involved in freeze-etch or freeze-fracture studies that it would be useless to etch specimens which were cryoprotected by more than 15% glycerol. We presumed that the amount of cryoprotective material exposed at the surface would serve as a contaminating layer and prevent the visualization of fine details. Recent unexpected freeze-etch results indicated that it would be useful to compare complementary replicas in which one-half of the frozen-fractured specimen would be shadowed and replicated immediately after fracturing whereas the complement would be etched at -98°C for 1 to 10 minutes before being shadowed and replicated.Standard complementary replica holders (Steere, 1973) with hinges removed were used for this study. Specimens consisting of unfixed virus-infected plant tissue infiltrated with 0.05 M phosphate buffer or distilled water were used without cryoprotectant. Some were permitted to settle through gradients to the desired concentrations of different cryoprotectants.

Freeze-Fracture Studies of Membranes in Developing Sieve Elements in a Plant Tissue Culture

1980 ◽  
Vol 38 ◽  
pp. 636-637
Author(s):  
R. D. Sjolund ◽  
C. Y. Shih
Keyword(s):  
Plant Tissue ◽  
Cultured Cells ◽  
Freeze Fracture ◽  
Tissue Cultures ◽  
Sieve Elements ◽  
Intact Plants ◽  
Plant Tissue Cultures ◽  
Whole Plants ◽  
Energy Dependent ◽  
Similar Elements

The differentiation of phloem in plant tissue cultures offers a unique opportunity to study the development and structure of sieve elements in a manner that avoids the injury responses associated with the processing of similar elements in intact plants. Short segments of sieve elements formed in tissue cultures can be fixed intact while the longer strands occuring in whole plants must be cut into shorter lengths before processing. While iyuch controversy surrounds the question of phloem function in tissue cultures , sieve elements formed in these cultured cells are structurally similar to those of Intact plants. We are particullarly Interested In the structure of the plasma membrane and the peripheral ER in these cells because of their possible role in the energy-dependent active transport of sucrose into the sieve elements.

Development of Plant Tissue Culture Profile of Dioscorea deltoidea

Planta Medica ◽  
2013 ◽  
Vol 79 (05) ◽  
Author(s):  
M Mujeeb ◽  
M Amir ◽  
AS Nadeem ◽  
M Aqil ◽  
AK Najmi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Dioscorea Deltoidea
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