scholarly journals Three‐Dimensional Fruit Tissue Habitats for Culturing Caenorhabditis elegans

2021 ◽  
Vol 1 (11) ◽  
Author(s):  
Aurélie Guisnet ◽  
Malosree Maitra ◽  
Sreeparna Pradhan ◽  
Michael Hendricks
Keyword(s):  
Caenorhabditis Elegans ◽  
Three Dimensional ◽  
Fruit Tissue

scholarly journals Label-free three-dimensional imaging of Caenorhabditis elegans with visible optical coherence microscopy

PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0181676 ◽  
Author(s):  
Séverine Coquoz ◽  
Paul J. Marchand ◽  
Arno Bouwens ◽  
Laurent Mouchiroud ◽  
Vincenzo Sorrentino ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Three Dimensional ◽  
Optical Coherence ◽  
Label Free ◽  
Optical Coherence Microscopy ◽  
Three Dimensional Imaging

scholarly journals Superresolution microscopy reveals the three-dimensional organization of meiotic chromosome axes in intact Caenorhabditis elegans tissue

2017 ◽  
Vol 114 (24) ◽  
pp. E4734-E4743 ◽  
Author(s):  
Simone Köhler ◽  
Michal Wojcik ◽  
Ke Xu ◽  
Abby F. Dernburg
Keyword(s):  
Caenorhabditis Elegans ◽  
Fluorescent Protein ◽  
Germ Line ◽  
Meiotic Chromosome ◽  
Three Dimensional ◽  
Three Dimensions ◽  
Molecular Organization ◽  
Superresolution Microscopy ◽  
Specific Proteins ◽  
Optical Reconstruction

When cells enter meiosis, their chromosomes reorganize as linear arrays of chromatin loops anchored to a central axis. Meiotic chromosome axes form a platform for the assembly of the synaptonemal complex (SC) and play central roles in other meiotic processes, including homologous pairing, recombination, and chromosome segregation. However, little is known about the 3D organization of components within the axes, which include cohesin complexes and additional meiosis-specific proteins. Here, we investigate the molecular organization of meiotic chromosome axes in Caenorhabditis elegans through STORM (stochastic optical reconstruction microscopy) and PALM (photo-activated localization microscopy) superresolution imaging of intact germ-line tissue. By tagging one axis protein (HIM-3) with a photoconvertible fluorescent protein, we established a spatial reference for other components, which were localized using antibodies against epitope tags inserted by CRISPR/Cas9 genome editing. Using 3D averaging, we determined the position of all known components within synapsed chromosome axes to high spatial precision in three dimensions. We find that meiosis-specific HORMA domain proteins span a gap between cohesin complexes and the central region of the SC, consistent with their essential roles in SC assembly. Our data further suggest that the two different meiotic cohesin complexes are distinctly arranged within the axes: Although cohesin complexes containing the kleisin REC-8 protrude above and below the plane defined by the SC, complexes containing COH-3 or -4 kleisins form a central core, which may physically separate sister chromatids. This organization may help to explain the role of the chromosome axes in promoting interhomolog repair of meiotic double-strand breaks by inhibiting intersister repair.

scholarly journals Three-dimensional simulation of the Caenorhabditis elegans body and muscle cells in liquid and gel environments for behavioural analysis

2018 ◽  
Vol 373 (1758) ◽  
pp. 20170376 ◽  
Author(s):  
Andrey Palyanov ◽  
Sergey Khayrulin ◽  
Stephen D. Larson
Keyword(s):  
Caenorhabditis Elegans ◽  
Muscle Activation ◽  
Body Wall ◽  
Particle System ◽  
Three Dimensional ◽  
Muscle Cells ◽  
The Body ◽  
C Elegans ◽  
Muscle Activation Patterns ◽  
Simulation Engine

To better understand how a nervous system controls the movements of an organism, we have created a three-dimensional computational biomechanical model of the Caenorhabditis elegans body based on real anatomical structure. The body model is created with a particle system–based simulation engine known as Sibernetic, which implements the smoothed particle–hydrodynamics algorithm. The model includes an elastic body-wall cuticle subject to hydrostatic pressure. This cuticle is then driven by body-wall muscle cells that contract and relax, whose positions and shape are mapped from C. elegans anatomy, and determined from light microscopy and electron micrograph data. We show that by using different muscle activation patterns, this model is capable of producing C. elegans -like behaviours, including crawling and swimming locomotion in environments with different viscosities, while fitting multiple additional known biomechanical properties of the animal.  This article is part of a discussion meeting issue ‘Connectome to behaviour: modelling C. elegans at cellular resolution’.

scholarly journals The Caenorhabditis elegans Dosage Compensation Machinery Is Recruited to X Chromosome DNA Attached to an Autosome

Genetics ◽  
2000 ◽  
Vol 156 (4) ◽  
pp. 1603-1621
Author(s):  
Jason D Lieb ◽  
Carlos Ortiz de Solorzano ◽  
Enrique Garcia Rodriguez ◽  
Arthur Jones ◽  
Michael Angelo ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
X Chromosome ◽  
Dosage Compensation ◽  
Dna Sequences ◽  
Three Dimensional ◽  
Dosage Compensation Complex ◽  
X Chromosomes ◽  
Cis Acting ◽  
Recognition Elements ◽  
The Right

Abstract The dosage compensation machinery of Caenorhabditis elegans is targeted specifically to the X chromosomes of hermaphrodites (XX) to reduce gene expression by half. Many of the trans-acting factors that direct the dosage compensation machinery to X have been identified, but none of the proposed cis-acting X chromosome-recognition elements needed to recruit dosage compensation components have been found. To study X chromosome recognition, we explored whether portions of an X chromosome attached to an autosome are competent to bind the C. elegans dosage compensation complex (DCC). To do so, we devised a three-dimensional in situ approach that allowed us to compare the volume, position, and number of chromosomal and subchromosomal bodies bound by the dosage compensation machinery in wild-type XX nuclei and XX nuclei carrying an X duplication. The dosage compensation complex was found to associate with a duplication of the right 30% of X, but the complex did not spread onto adjacent autosomal sequences. This result indicates that all the information required to specify X chromosome identity resides on the duplication and that the dosage compensation machinery can localize to a site distinct from the full-length hermaphrodite X chromosome. In contrast, smaller duplications of other regions of X appeared to not support localization of the DCC. In a separate effort to identify cis-acting X recognition elements, we used a computational approach to analyze genomic DNA sequences for the presence of short motifs that were abundant and overrepresented on X relative to autosomes. Fourteen families of X-enriched motifs were discovered and mapped onto the X chromosome.

Caenopore-5: The three-dimensional structure of an antimicrobial protein from Caenorhabditis elegans

2010 ◽  
Vol 34 (3) ◽  
pp. 323-330 ◽  
Author(s):  
Justyna Mysliwy ◽  
Andrew J. Dingley ◽  
Mareike Stanisak ◽  
Sascha Jung ◽  
Inken Lorenzen ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Antimicrobial Protein ◽  
Three Dimensional Structure

Computational prediction of the three-dimensional structures for the Caenorhabditis elegans tubulin family

1999 ◽  
Vol 17 (2) ◽  
pp. 90-100 ◽  
Author(s):  
Camelia B Gogonea ◽  
Valentin Gogonea ◽  
Yusuf M Ali ◽  
Kenneth M Merz ◽  
Shahid S Siddiqui
Keyword(s):  
Caenorhabditis Elegans ◽  
Three Dimensional ◽  
Computational Prediction
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