Single‐Cell Sorting of Non‐human Primate Adipocytes with Large‐particle Flow Cytometry

2021 ◽  
Vol 1 (11) ◽  
Author(s):  
Jacob J. Robino ◽  
Yongwoon Kim ◽  
Oleg Varlamov
Keyword(s):  
Flow Cytometry ◽  
Single Cell ◽  
Cell Sorting ◽  
Large Particle ◽  
Particle Flow ◽  
Human Primate

scholarly journals Large particle fluorescence-activated cell sorting enables high quality single cell RNA-sequencing and functional analysis of adult cardiomyocytes

2019 ◽  
Author(s):  
Suraj Kannan ◽  
Matthew Miyamoto ◽  
Brian Lin ◽  
Renjun Zhu ◽  
Sean Murphy ◽  
...  
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Cell Sorting ◽  
Large Particle ◽  
Fluorescence Activated Cell Sorting ◽  
High Quality ◽  
Adult Cardiomyocytes ◽  
Single Cell Rna Sequencing ◽  
Mitochondrial Transcripts ◽  
Manual Methods

ABSTRACTRationaleSingle cell RNA sequencing (scRNA-seq) has emerged as a powerful tool to profile the transcriptome at single cell resolution, enabling comprehensive analysis of cellular trajectories and heterogeneity during development and disease. However, the use of scRNA-seq remains limited in cardiac pathology owing to technical difficulties associated with the isolation of single adult cardiomyocytes (CMs).ObjectiveWe investigated the capability of large-particle fluorescence-activated cell sorting (LP-FACS) for isolation of viable single adult CMs.Methods and ResultsWe found that LP-FACS readily outperforms conventional FACS for isolation of struturally competent CMs, including large CMs. Additionally, LP-FACS enables isolation of fluorescent CMs from mosaic models. Importantly, the sorted CMs allow generation of high-quality scRNA-seq libraries. Unlike CMs isolated via previously utilized fluidic or manual methods, LP-FAC-isolated CMs generate libraries exhibiting normal levels of mitochondrial transcripts. Moreover, LP-FACS isolated CMs remain functionally competent and can be studied for contractile properties.ConclusionsOur study enables high quality dissection of adult CM biology at single-cell resolution.

scholarly journals Improvement of phytoplankton culture isolation using single cell sorting by flow cytometry

2017 ◽  
Vol 53 (2) ◽  
pp. 271-282 ◽  
Author(s):  
Dominique Marie ◽  
Florence Le Gall ◽  
Roseline Edern ◽  
Priscillia Gourvil ◽  
Daniel Vaulot
Keyword(s):  
Flow Cytometry ◽  
Single Cell ◽  
Cell Sorting

scholarly journals Flow Cytometry Combined With Single Cell Sorting to Study Heterogeneous Germination of Bacillus Spores Under High Pressure

2020 ◽  
Vol 10 ◽  
Author(s):  
Yifan Zhang ◽  
Alessia I. Delbrück ◽  
Cosima L. Off ◽  
Stephan Benke ◽  
Alexander Mathys
Keyword(s):  
Flow Cytometry ◽  
High Pressure ◽  
Single Cell ◽  
Cell Sorting ◽  
Bacillus Spores

scholarly journals A high throughput method for quantifying number and size distribution of Arabidopsis seeds using large particle flow cytometry

Plant Methods ◽  
2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Alejandro Morales ◽  
J. Teapal ◽  
J. M. H. Ammerlaan ◽  
X. Yin ◽  
J. B. Evers ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Size Distribution ◽  
High Throughput ◽  
Large Particle ◽  
Particle Flow ◽  
High Throughput Method
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