High‐Throughput Computational Analysis of Biofilm Formation from Time‐Lapse Microscopy

2021 ◽  
Vol 1 (7) ◽  
Author(s):  
Ola E. Salama ◽  
Aleeza C. Gerstein
Keyword(s):  
High Throughput ◽  
Biofilm Formation ◽  
Computational Analysis ◽  
Time Lapse ◽  
Time Lapse Microscopy

Label free high throughput screening for apoptosis inducing chemicals using time-lapse microscopy signal processing

APOPTOSIS ◽  
2014 ◽  
Vol 19 (9) ◽  
pp. 1411-1418 ◽  
Author(s):  
Obaid Aftab ◽  
Madiha Nazir ◽  
Mårten Fryknäs ◽  
Ulf Hammerling ◽  
Rolf Larsson ◽  
...  
Keyword(s):  
Signal Processing ◽  
High Throughput ◽  
High Throughput Screening ◽  
Time Lapse ◽  
Label Free ◽  
Time Lapse Microscopy

scholarly journals Isolating live cells after high-throughput, long-term, time-lapse microscopy

2019 ◽  
Vol 17 (1) ◽  
pp. 93-100 ◽  
Author(s):  
Scott Luro ◽  
Laurent Potvin-Trottier ◽  
Burak Okumus ◽  
Johan Paulsson
Keyword(s):  
High Throughput ◽  
Time Lapse ◽  
Live Cells ◽  
Time Lapse Microscopy

scholarly journals Antibody-Mediated Immobilization of Cryptococcus neoformans Promotes Biofilm Formation

2009 ◽  
Vol 75 (8) ◽  
pp. 2528-2533 ◽  
Author(s):  
Emma J. Robertson ◽  
Arturo Casadevall
Keyword(s):  
Monoclonal Antibody ◽  
Cryptococcus Neoformans ◽  
Biofilm Formation ◽  
Capsular Polysaccharide ◽  
Time Lapse ◽  
Biofilm Growth ◽  
Time Lapse Microscopy ◽  
Antibody Capture ◽  
Pathogenic Microbes ◽  
Antibody Coating

ABSTRACT Most microbes, including the fungal pathogen Cryptococcus neoformans, can grow as biofilms. Biofilms confer upon microbes a range of characteristics, including an ability to colonize materials such as shunts and catheters and increased resistance to antibiotics. Here, we provide evidence that coating surfaces with a monoclonal antibody to glucuronoxylomannan, the major component of the fungal capsular polysaccharide, immobilizes cryptococcal cells to a surface support and, subsequently, promotes biofilm formation. We used time-lapse microscopy to visualize the growth of cryptococcal biofilms, generating the first movies of fungal biofilm growth. We show that when fungal cells are immobilized using surface-attached specific antibody to the capsule, the initial stages of biofilm formation are significantly faster than those on surfaces with no antibody coating or surfaces coated with unspecific monoclonal antibody. Time-lapse microscopy revealed that biofilm growth was a dynamic process in which cells shuffled position during budding and was accompanied by emergence of planktonic variant cells that left the attached biofilm community. The planktonic variant cells exhibited mobility, presumably by Brownian motion. Our results indicate that microbial immobilization by antibody capture hastens biofilm formation and suggest that antibody coating of medical devices with immunoglobulins must exclude binding to common pathogenic microbes and the possibility that this effect could be exploited in industrial microbiology.

Experience of using time-lapse microscopy in IVF and ICSI programs

2020 ◽  
pp. 47-50
Author(s):  
N. V. Saraeva ◽  
N. V. Spiridonova ◽  
M. T. Tugushev ◽  
O. V. Shurygina ◽  
A. I. Sinitsyna
Keyword(s):  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Selection Procedure ◽  
Time Lapse ◽  
Single Embryo Transfer ◽  
Main Group ◽  
Clinical Pregnancy ◽  
Control Group ◽  
Time Lapse Microscopy

In order to increase the pregnancy rate in the assisted reproductive technology, the selection of one embryo with the highest implantation potential it is very important. Time-lapse microscopy (TLM) is a tool for selecting quality embryos for transfer. This study aimed to assess the benefits of single-embryo transfer of autologous oocytes performed on day 5 of embryo incubation in a TLM-equipped system in IVF and ICSI programs. Single-embryo transfer following incubation in a TLM-equipped incubator was performed in 282 patients, who formed the main group; the control group consisted of 461 patients undergoing single-embryo transfer following a traditional culture and embryo selection procedure. We assessed the quality of transferred embryos, the rates of clinical pregnancy and delivery. The groups did not differ in the ratio of IVF and ICSI cycles, average age, and infertility factor. The proportion of excellent quality embryos for transfer was 77.0% in the main group and 65.1% in the control group (p = 0.001). In the subgroup with receiving eight and less oocytes we noted the tendency of receiving more quality embryos in the main group (р = 0.052). In the subgroup of nine and more oocytes the quality of the transferred embryos did not differ between two groups. The clinical pregnancy rate was 60.2% in the main group and 52.9% in the control group (p = 0.057). The delivery rate was 45.0% in the main group and 39.9% in the control group (p > 0.050).

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