scholarly journals Synthesis of DNA and RNA Oligonucleotides Containing a Dual‐Purpose Selenium‐Modified Fluorescent Nucleoside Probe

2020 ◽  
Vol 81 (1) ◽  
Author(s):  
Ashok Nuthanakanti ◽  
Seergazhi G. Srivatsan
Keyword(s):  
Dual Purpose ◽  
Dna And Rna ◽  
Fluorescent Nucleoside

scholarly journals Probing Human Telomeric DNA and RNA Topology and Ligand Binding in a Cellular Model by Using Responsive Fluorescent Nucleoside Probes

ChemBioChem ◽  
2017 ◽  
Vol 18 (16) ◽  
pp. 1604-1615 ◽  
Author(s):  
Sudeshna Manna ◽  
Cornelia H. Panse ◽  
Vyankat A. Sontakke ◽  
Sarangamath Sangamesh ◽  
Seergazhi G. Srivatsan
Keyword(s):  
Ligand Binding ◽  
Cellular Model ◽  
Telomeric Dna ◽  
Dna And Rna ◽  
Fluorescent Nucleoside

scholarly journals Assessing G4-Binding Ligands In Vitro and in Cellulo Using Dimeric Carbocyanine Dye Displacement Assay

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1400
Author(s):  
Nakshi Desai ◽  
Viraj Shah ◽  
Bhaskar Datta
Keyword(s):  
Confocal Microscopy ◽  
Fluorescence Enhancement ◽  
Cell Permeability ◽  
Live Cells ◽  
Dual Purpose ◽  
Primary Screening ◽  
Dna And Rna ◽  
Carbocyanine Dye ◽  
Displacement Assay

G-quadruplexes (G4) are the most actively studied non-canonical secondary structures formed by contiguous repeats of guanines in DNA or RNA strands. Small molecule mediated targeting of G-quadruplexes has emerged as an attractive tool for visualization and stabilization of these structures inside the cell. Limited number of DNA and RNA G4-selective assays have been reported for primary ligand screening. A combination of fluorescence spectroscopy, AFM, CD, PAGE, and confocal microscopy have been used to assess a dimeric carbocyanine dye B6,5 for screening G4-binding ligands in vitro and in cellulo. The dye B6,5 interacts with physiologically relevant DNA and RNA G4 structures, resulting in fluorescence enhancement of the molecule as an in vitro readout for G4 selectivity. Interaction of the dye with G4 is accompanied by quadruplex stabilization that extends its use in primary screening of G4 specific ligands. The molecule is cell permeable and enables visualization of quadruplex dominated cellular regions of nucleoli using confocal microscopy. The dye is displaced by quarfloxin in live cells. The dye B6,5 shows remarkable duplex to quadruplex selectivity in vitro along with ligand-like stabilization of DNA G4 structures. Cell permeability and response to RNA G4 structures project the dye with interesting theranostic potential. Our results validate that B6,5 can serve the dual purpose of visualization of DNA and RNA G4 structures and screening of G4 specific ligands, and adds to the limited number of probes with such potential.

High-resolution nucleic acid sequence mapping via in situ hybridization at the Electron Microscope level

1995 ◽  
Vol 53 ◽  
pp. 752-753
Author(s):  
B.A. Hamkalo ◽  
S. Narayanswami ◽  
A.P. Kausch
Keyword(s):  
Nucleic Acid ◽  
Interphase Nucleus ◽  
Genome Mapping ◽  
Precise Location ◽  
Electron Microscope Level ◽  
Rna Sequences ◽  
Fundamental Interest ◽  
Whole Cells ◽  
Dna And Rna

The availability of nonradioactive methods to label nucleic acids an the resultant rapid and greater sensitivity of detection has catapulted the technique of in situ hybridization to become the method of choice to locate of specific DNA and RNA sequences on chromosomes and in whole cells in cytological preparations in many areas of biology. It is being applied to problems of fundamental interest to basic cell and molecular biologists such as the organization of the interphase nucleus in the context of putative functional domains; it is making major contributions to genome mapping efforts; and it is being applied to the analysis of clinical specimens. Although fluorescence detection of nucleic acid hybrids is routinely used, certain questions require greater resolution. For example, very closely linked sequences may not be separable using fluorescence; the precise location of sequences with respect to chromosome structures may be below the resolution of light microscopy(LM); and the relative positions of sequences on very small chromosomes may not be feasible.

Scanning tunneling microscopy (STM) of DNA and RNA

1989 ◽  
Vol 47 ◽  
pp. 34-35
Author(s):  
Patricia G. Arscott ◽  
Gil Lee ◽  
Victor A. Bloomfield ◽  
D. Fennell Evans
Keyword(s):  
Molecular Structures ◽  
Inorganic Materials ◽  
Resolving Power ◽  
Scanning Tunneling ◽  
Tunneling Microscopy ◽  
Form And Function ◽  
Dna And Rna ◽  
Calf Thymus ◽  
And Function ◽  
The Relationship

STM is one of the most promising techniques available for visualizing the fine details of biomolecular structure. It has been used to map the surface topography of inorganic materials in atomic dimensions, and thus has the resolving power not only to determine the conformation of small molecules but to distinguish site-specific features within a molecule. That level of detail is of critical importance in understanding the relationship between form and function in biological systems. The size, shape, and accessibility of molecular structures can be determined much more accurately by STM than by electron microscopy since no staining, shadowing or labeling with heavy metals is required, and there is no exposure to damaging radiation by electrons. Crystallography and most other physical techniques do not give information about individual molecules.We have obtained striking images of DNA and RNA, using calf thymus DNA and two synthetic polynucleotides, poly(dG-me5dC)·poly(dG-me5dC) and poly(rA)·poly(rU).

Aerospace monitoring of the Ukrainian near-shelf areas of the Black Sea as a dual purpose methodology

2018 ◽  
Vol 04 ◽  
pp. 68-75 ◽  
Author(s):  
O.A. Shchypsov ◽  
◽  
O.D. Fedorovsky ◽  
A.V. Khyzhniak ◽  
◽  
...  
Keyword(s):  
Black Sea ◽  
The Black Sea ◽  
Dual Purpose ◽  
Aerospace Monitoring
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