Digital Droplet PCR for Monitoring Tissue‐Specific Cell Death Using DNA Methylation Patterns of Circulating Cell‐Free DNA

2019 ◽  
Vol 127 (1) ◽  
Author(s):  
Ruth Shemer ◽  
Judith Magenheim ◽  
Yuval Dor
Keyword(s):  
Dna Methylation ◽  
Cell Death ◽  
Specific Cell ◽  
Cell Free Dna ◽  
Tissue Specific ◽  
Digital Droplet Pcr ◽  
Free Dna ◽  
Droplet Pcr ◽  
Methylation Patterns ◽  
Circulating Cell Free Dna

scholarly journals Aging-associated distinctive DNA methylation changes of LINE-1 retrotransposons in pure cell-free DNA from human blood

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Wardah Mahmood ◽  
Lars Erichsen ◽  
Pauline Ott ◽  
Wolfgang A. Schulz ◽  
Johannes C. Fischer ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Cell Free Dna ◽  
The Past ◽  
Free Dna ◽  
Genome Wide ◽  
Cancerous Cell ◽  
Pure Cell ◽  
Epigenetic Biomarker ◽  
Methylation Patterns ◽  
Cancerous Cells

AbstractLINE-1 hypomethylation of cell-free DNA has been described as an epigenetic biomarker of human aging. However, in the past, insufficient differentiation between cellular and cell-free DNA may have confounded analyses of genome-wide methylation levels in aging cells. Here we present a new methodological strategy to properly and unambiguously extract DNA methylation patterns of repetitive, as well as single genetic loci from pure cell-free DNA from peripheral blood. Since this nucleic acid fraction originates mainly in apoptotic, senescent and cancerous cells, this approach allows efficient analysis of aged and cancerous cell-specific DNA methylation patterns for diagnostic and prognostic purposes. Using this methodology, we observe a significant age-associated erosion of LINE-1 methylation in cfDNA suggesting that the threshold of hypomethylation sufficient for relevant LINE-1 activation and consequential harmful retrotransposition might be reached at higher age. We speculate that this process might contribute to making aging the main risk factor for many cancers.

scholarly journals Monitoring EGFR C797S mutation in Japanese NSCLC patients with serial cell free DNA evaluation using digital droplet PCR

2021 ◽  
Author(s):  
Ryo Ariyasu ◽  
Ken Uchibori ◽  
Takaaki Sasaki ◽  
Mika Tsukahara ◽  
Kazuma Kiyotani ◽  
...  
Keyword(s):  
Cell Free Dna ◽  
Digital Droplet Pcr ◽  
Free Dna ◽  
Droplet Pcr ◽  
Nsclc Patients

scholarly journals Comparison of quantification algorithms for circulating cell-free DNA methylation biomarkers in blood plasma from cancer patients

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Luka de Vos ◽  
Heidrun Gevensleben ◽  
Andreas Schröck ◽  
Alina Franzen ◽  
Glen Kristiansen ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Blood Plasma ◽  
Cancer Patients ◽  
Cell Free Dna ◽  
Free Dna ◽  
In Blood Plasma ◽  
Circulating Cell Free Dna

Circulating cell-free DNA in breast cancer: size profiling, levels, and methylation patterns lead to prognostic and predictive classifiers

Oncogene ◽  
2019 ◽  
Vol 38 (18) ◽  
pp. 3387-3401 ◽  
Author(s):  
Maria Panagopoulou ◽  
Makrina Karaglani ◽  
Ioanna Balgkouranidou ◽  
Eirini Biziota ◽  
Triantafillia Koukaki ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Free Dna ◽  
Free Dna ◽  
Methylation Patterns ◽  
Circulating Cell Free Dna
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