Isolation and Characterization of Extracellular Vesicles from Cell Culture Conditioned Medium for Immunological Studies

2020 ◽  
Vol 129 (1) ◽  
Author(s):  
Julian Swatler ◽  
Wioleta Dudka ◽  
Katarzyna Piwocka
Keyword(s):  
Cell Culture ◽  
Conditioned Medium ◽  
Extracellular Vesicles ◽  
Isolation And Characterization

Isolation and characterization of extracellular vesicles

2019 ◽  
Vol 1 (1) ◽  
pp. 18-26
Author(s):  
Fabia Fricke ◽  
Dominik Buschmann ◽  
Michael W. Pfaffl
Keyword(s):  
Cell Culture ◽  
Extracellular Vesicles ◽  
Culture Media ◽  
Body Fluids ◽  
Cell Culture Media ◽  
Advantages And Disadvantages ◽  
The Past ◽  
Isolation And Characterization ◽  
Basic Biology

Research into extracellular vesicles (EVs) gained significant traction in the past decade and EVs have been investigated in a wide variety of studies ranging from basic biology to diagnostic and therapeutic applications. Since EVs are secreted by most, if not all, eukaryotic and prokaryotic cells, they have been detected in body fluids as diverse as blood, urine and saliva as well as in cell culture media. In this chapter, we will provide an overview of EV isolation and characterization strategies and highlight their advantages and disadvantages.

scholarly journals Isolation and characterization of extracellular vesicles from Caenorhabditis elegans for multi-omic analysis

2018 ◽  
Author(s):  
Joshua C. Russell ◽  
Gennifer E. Merrihew ◽  
Julia E. Robbins ◽  
Nadia Postupna ◽  
Tyek-Kyun Kim ◽  
...  
Keyword(s):  
Cell Culture ◽  
Caenorhabditis Elegans ◽  
Extracellular Vesicles ◽  
Mammalian Cell Culture ◽  
Model Systems ◽  
C Elegans ◽  
Isolation And Characterization ◽  
Transmission Electron ◽  
Invertebrate Model

AbstractCells from bacteria to human release vesicles into their extracellular environment. These extracellular vesicles (EVs) contain multiple classesof molecules, including nucleic acids, proteins, and lipids. The isolation and analysis of EV cargos from mammalian cell culture and liquid biopsysamples has become a powerful approach for uncovering the messages that are packaged into these organelles. However, this approach has not been tenable in invertebrate model systems due to lack of sufficient amounts of pure EVs. Here we report a robust and reproducible procedure to isolateEVs from Caenorhabditis elegans with yields similar to those obtained from human cell culture. Through nanoparticle tracking, transmission electron microscopy, flow cytometry, mass spectrometry, RNAseq, and immunoaffinity analysis we provide the first ever detailed characterization of C. elegans EV composition and demonstrate that C. elegans EVs share fundamentally similar properties with their mammalian counterparts. These include vesicle size, enrichment for lipid rafts, and similar types of RNA and protein cargos. This ability of isolate pure EVs on ascale amenable to multiple types of downstream analyses permits, multi-omics characterization of EV cargos in an invertebrate model system.

scholarly journals 109 Isolation and characterization of myometrium and decidual cell-derived extracellular vesicles

2021 ◽  
Vol 224 (2) ◽  
pp. S75-S76
Author(s):  
Megan Shepherd ◽  
Enkhtuya Radnaa ◽  
Rheanna Urrabaz-Garza ◽  
Talar Kechichian ◽  
Ourlad Alzeus G. Tantengco ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Decidual Cell ◽  
Isolation And Characterization

scholarly journals Emerging techniques in the isolation and characterization of extracellular vesicles and their roles in cancer diagnostics and prognostics

The Analyst ◽  
2016 ◽  
Vol 141 (2) ◽  
pp. 371-381 ◽  
Author(s):  
Vijaya Sunkara ◽  
Hyun-Kyung Woo ◽  
Yoon-Kyoung Cho
Keyword(s):  
Extracellular Vesicles ◽  
Cancer Diagnostics ◽  
Clinical Implementation ◽  
Characterization Methods ◽  
Cancer Management ◽  
Isolation And Characterization

We present an overview of current isolation, detection, and characterization methods of extracellular vesicles and their applications and limitations as a potential emerging biomarker in cancer management and their clinical implementation.

Isolation and Characterization of Extracellular Vesicles in Stem Cell-Related Studies

2017 ◽  
pp. 205-223
Author(s):  
Zezhou Zhao ◽  
Dillon C. Muth ◽  
Vasiliki Mahairaki ◽  
Linzhao Cheng ◽  
Kenneth W. Witwer
Keyword(s):  
Stem Cell ◽  
Extracellular Vesicles ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of canine parvovirus type 2c circulating in Uruguay

2011 ◽  
Vol 41 (8) ◽  
pp. 1436-1440 ◽  
Author(s):  
Andrea Blanc Pintos ◽  
Cecilia Beatriz Negro Larrama ◽  
Eduardo Enrique Reolon Baratta ◽  
Mabel Beatriz Berois Barthe ◽  
Juan Ramón Arbiza Rodonz
Keyword(s):  
Cell Culture ◽  
Restriction Fragment Length Polymorphism ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Gene Encoding ◽  
Partial Sequencing ◽  
Phylogenetic Characterization ◽  
Isolation And Characterization ◽  
Restriction Fragment Length

This research reports the first CPV-2c isolation in cell culture (canine fibroma cell line A-72) in Uruguay. The isolates were obtained from 13 rectal swabs of Uruguayan dogs with parvovirosis. Samples were submitted to PCR with two sets of primers, restriction fragment length polymorphism (RFLP), partial sequencing of the gene encoding for VP2 capsid protein and phylogenetic characterization. The strain isolated was confirmed as CPV-2c. These results contribute to a better knowledge of CPV strains circulating in Uruguay and promote an evaluation of the efficacy of heterologous vaccines used to protect against the circulating strains.

scholarly journals Isolation and characterization of hantavirus carried by Apodemus peninsulae in Jilin, China

2007 ◽  
Vol 88 (4) ◽  
pp. 1295-1301 ◽  
Author(s):  
Yong-Zhen Zhang ◽  
Yang Zou ◽  
Lai-Shun Yao ◽  
Guang-Wei Hu ◽  
Zhan-Shen Du ◽  
...  
Keyword(s):  
Cell Culture ◽  
Phylogenetic Analysis ◽  
Eastern China ◽  
Immunofluorescence Assay ◽  
Haemorrhagic Fever ◽  
Apodemus Peninsulae ◽  
Isolation And Characterization ◽  
North Eastern ◽  
Field Mice

To provide a better understanding of hantavirus epidemiology in China, Korean field mice (Apodemus peninsulae) and striped field mice (Apodemus agrarius) were captured in Jilin province, China, where haemorrhagic fever with renal syndrome (HFRS) is endemic. Hantavirus antigens were detected in eight of the 130 A. peninsulae individuals and in four of the 193 A. agrarius individuals by using an immunofluorescence assay. Partial S and M segments were amplified from all of the antigen-positive samples. Furthermore, two hantaviruses (CJAp89 and CJAp93) were isolated successfully in cell culture and the entire S and M segments were amplified from one of them (CJAp93). Phylogenetic analysis of these sequences (partial or complete) showed that hantaviruses carried by A. peninsulae and A. agrarius form two distinct lineages, although viruses carried by A. peninsulae are similar to those isolated previously from A. agrarius in China and from HFRS patients in Russia. However, the viruses detected in A. peninsulae in China are genetically different from those detected in A. peninsulae in other countries. These data suggest that A. peninsulae is also a natural host for HTNV in north-eastern China.

scholarly journals Isolation and characterization of platelet-derived extracellular vesicles

2014 ◽  
Vol 3 (1) ◽  
pp. 24692 ◽  
Author(s):  
Maria T. Aatonen ◽  
Tiina Öhman ◽  
Tuula A. Nyman ◽  
Saara Laitinen ◽  
Mikaela Grönholm ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Isolation And Characterization

Isolation and characterization of a neu protein-specific activating factor from human ATL-2 cell conditioned medium

1991 ◽  
Vol 179 (3) ◽  
pp. 1536-1542 ◽  
Author(s):  
James G. Davis ◽  
Junji Hamuro ◽  
Caroline Y. Shim ◽  
Arabinda Samanta ◽  
Mark I. Greene ◽  
...  
Keyword(s):  
Conditioned Medium ◽  
Isolation And Characterization ◽  
Cell Conditioned Medium
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