Release of free fatty acids from adipose tissue in vitro in relation to rates of triglyceride synthesis and degradation

2011 ◽  
Author(s):  
Daniel Steinberg ◽  
Martha Vaughan
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Free Fatty Acids ◽  
Triglyceride Synthesis ◽  
Adipose Tissue In Vitro ◽  
Synthesis And Degradation

Effect of nicotine on the mobilization of free fatty acids from adipose tissue in vitro

1969 ◽  
Vol 25 (2) ◽  
pp. 128-128 ◽  
Author(s):  
A. Kershbaum ◽  
H. Osada ◽  
D. J. Pappajohn ◽  
S. Bellet
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Free Fatty Acids ◽  
Adipose Tissue In Vitro

Effect of β-adrenergic agonists on lipolysis and lipogenesis by porcine adipose tissue in vitro

1990 ◽  
Vol 68 (4) ◽  
pp. 1024-1029 ◽  
Author(s):  
T. A. Peterla ◽  
C. G. Scanes
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Free Fatty Acids ◽  
Direct Effects ◽  
Adrenergic Agonists ◽  
Tissue Explants ◽  
In Vitro Lipolysis ◽  
Adipose Tissue In Vitro ◽  
Release Of Glycerol

Abstract The effects of the β-adrenergic agonists isoproterenol, cimaterol, ractopamine and clenbuterol on lipolysis (release of glycerol and free fatty acids) and lipogenesis (incorporation of 14C into fatty acids from [14C]glucose) was examined in porcine adipose tissue explants in vitro. Lipolysis was stimulated by isoproterenol, cimaterol or ractopamine but not by clenbuterol. Insulin reduced the lipolytic effects of the β-adrenergic agonists (isoproterenol, cimaterol and ractopamine). Lipogenesis was inhibited by all β-adrenergic agonists tested (isoproterenol, cimaterol, ractopamine and clenbuterol). The antilipogenic effect of the β-adrenergic agonists was reduced by the presence of insulin in the incubation. Although effects of the different β-adrenergic agonists varied, all had some direct effects that could be expected to reduce adipose accretion. Effects of β-adrenergic agonists in the pig are due in part to direct effects on adipose tissue.

THE STIMULATING EFFECTS OF ADRENALINE AND ANTERIOR PITUITARY HORMONES ON THE RELEASE OF FREE FATTY ACIDS FROM ADIPOSE TISSUE

1962 ◽  
Vol 25 (2) ◽  
pp. 189-198 ◽  
Author(s):  
R. M. BUCKLE
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Pituitary Hormones ◽  
Thyroid Stimulating Hormone ◽  
Adrenocorticotrophic Hormone ◽  
Fat Pads

SUMMARY The quantity of free fatty acids (FFA) released from rat epididymal fat pads in vitro and their concentration within the tissue were determined. The addition of adrenaline, adrenocorticotrophic hormone (ACTH), thyroid stimulating hormone (TSH) and growth hormone (GH) each increased the release of FFA, and their respective minimum effective concentrations were 0·125, 0·004, 0·5 and 1·25 μg./ml. of medium. In every case, the increased release of FFA was associated with a rise in the quantity present within the pads, and the amount released closely paralleled their concentration within the tissue. It is suggested that the stimulatory effect of all four hormones on the release of FFA from adipose tissue is largely a manifestation of their activity of increasing the concentration of FFA within the cells, and this they do by facilitating the net conversion of storage triglyceride to fatty acid. The significance of the relative activities of the hormones in vitro is discussed and compared with their fatty acid mobilizing effects in vivo.

scholarly journals Fatty acids synthesis in rat adipose tissue. Tracer concentration effects in vitro

1968 ◽  
Vol 106 (1) ◽  
pp. 179-183 ◽  
Author(s):  
W. G. Duncombe
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Acid Synthesis ◽  
Long Chain Fatty Acids ◽  
Concentration Effects ◽  
Tracer Concentration ◽  
Adipose Tissue In Vitro ◽  
Rat Adipose Tissue ◽  
Long Chain

1. The use of labelled acetate for studying the synthesis of long-chain fatty acids in rat adipose tissue in vitro has been examined, with special reference to the effect of acetate concentration. 2. The incorporation of acetate into fatty acids is proportional to the concentration of acetate in the medium when the latter does not exceed about 10μm. Above this concentration, the relative incorporation becomes progressively less, and reasons for this are discussed. 3. In particular it is shown that this is not necessarily due to disturbance of the endogenous rate of fatty acid synthesis by a relatively large amount of acetyl-CoA derived from added acetate. 4. However, to ensure that the added acetate does not cause such a disturbance its concentration must be kept sufficiently low. For labelled acetate used under present conditions, this concentration should not be more than about 10μm.

THE EFFECT OF INSULIN ON THE UTILIZATION OF [U-14C]GLUCOSE AND [1-14C]ACETATE BY GOAT ADIPOSE TISSUE IN VITRO

1969 ◽  
Vol 44 (1) ◽  
pp. 115-119 ◽  
Author(s):  
J. ŠKARDA ◽  
S. BARTOŠ
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Glucose Oxidation ◽  
Marked Effect ◽  
Acetate Incorporation ◽  
Adipose Tissue In Vitro ◽  
High Concentrations

SUMMARY No change in the rate of 14CO2 production from [U-14C]glucose by the adipose tissue of goats was found in vitro, even in the presence of high concentrations of insulin (1 and 10 m-u./ml.) when glucose was the only substrate in the medium. However, it was demonstrated that in the presence of acetate as little as 10 μu. insulin/ml. exerted a marked effect on glucose oxidation. The most significant effect of insulin was that on the rate of [1-14C]acetate incorporation into fatty acids in the presence of glucose. These findings support the suggestion that the significance of insulin in ruminants is best demonstrated by its effects on the rate of utilization of acetate in the presence of glucose by adipose tissue.

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