Role of Ion Transport in Control of Apoptotic Cell Death

2012 ◽  
Author(s):  
Florian Lang ◽  
Else K. Hoffmann
Keyword(s):  
Cell Death ◽  
Ion Transport ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death

Role of Apoptotic Cell Death in Sepsis

2003 ◽  
Vol 35 (9) ◽  
pp. 585-592 ◽  
Author(s):  
Richard S. Hotchkiss ◽  
Kevin W. Tinsley ◽  
Irene E. Karl
Keyword(s):  
Cell Death ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death

scholarly journals The role of apoptotic cell death in cardiovascular disease

2001 ◽  
Vol 170 (2) ◽  
pp. 132-140 ◽  
Author(s):  
R. McLaughlin ◽  
C. J. Kelly ◽  
E. Kay ◽  
D. Bouchier-Hayes
Keyword(s):  
Cardiovascular Disease ◽  
Cell Death ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death

scholarly journals The role of apoptotic cell death in the radiosensitising effect of gemcitabine

2009 ◽  
Vol 101 (4) ◽  
pp. 628-636 ◽  
Author(s):  
B Pauwels ◽  
J B Vermorken ◽  
A Wouters ◽  
J Ides ◽  
S Van Laere ◽  
...  
Keyword(s):  
Cell Death ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death

Functional Validation of DownRegulated MicroRNAs in HeLa Cells Treated with Polyalthia longifolia Leaf Extract Using Different Microscopic Approaches: A Morphological Alteration-Based Validation

2019 ◽  
Vol 25 (05) ◽  
pp. 1263-1272 ◽  
Author(s):  
Shanmugapriya ◽  
Soundararajan Vijayarathna ◽  
Sreenivasan Sasidharan
Keyword(s):  
Cell Death ◽  
Electron Microscope ◽  
Hela Cells ◽  
Leaf Extract ◽  
Apoptotic Cell ◽  
Morphological Changes ◽  
Apoptotic Cell Death ◽  
Morphological Alteration ◽  
Polyalthia Longifolia

AbstractSeveral microscopy methods have been developed to assess the morphological changes in cells in the investigations of the mode of cell death in response to a stimulus. Our recent finding on the treatment of the IC50 concentration (26.67 μg/mL) of Polyalthia longifolia leaf extract indicated the induction of apoptotic cell death via the regulation of miRNA in HeLa cells. Hence, the current study was conducted to validate the function of these downregulated microRNAs in P. longifolia-treated HeLa cells using microscopic approaches. These include scanning electron microscope (SEM), transmission electron microscope (TEM), and acridine orange/propidium iodide (AO/PI)-based fluorescent microscopy techniques by observing the morphological alterations to cells after transfection with mimic miRNA. Interestingly, the morphological changes observed in this study demonstrated the apoptotic hallmarks, for instance, cell blebbing, cell shrinkage, cytoplasmic and nuclear condensation, vacuolization, cytoplasmic extrusion, and the formation of apoptotic bodies, which proved the role of dysregulated miRNAs in apoptotic HeLa cell death after treatment with the P. longifolia leaf extract. Conclusively, the current study proved the crucial role of downregulated miR-484 and miR-221-5p in the induction of apoptotic cell death in P. longifolia-treated HeLa cells using three approaches—SEM, TEM, and AO/PI-based fluorescent microscope.

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