Pancreatic Beta Cell Differentiation From Human Pluripotent Stem Cells

2018 ◽  
Vol 99 (1) ◽  
pp. e68 ◽  
Author(s):  
Lina Sui ◽  
Rudolph L. Leibel ◽  
Dieter Egli
Keyword(s):  
Stem Cells ◽  
Cell Differentiation ◽  
Beta Cell ◽  
Pluripotent Stem Cells ◽  
Pancreatic Beta Cell ◽  
Human Pluripotent Stem Cells ◽  
Pancreatic Beta Cell Differentiation

New insights into human beta cell biology using human pluripotent stem cells

2020 ◽  
Vol 103 ◽  
pp. 31-40 ◽  
Author(s):  
Nur Shabrina Amirruddin ◽  
Blaise Su Jun Low ◽  
Kok Onn Lee ◽  
E Shyong Tai ◽  
Adrian Kee Keong Teo
Keyword(s):  
Stem Cells ◽  
Beta Cell ◽  
Pluripotent Stem Cells ◽  
Cell Biology ◽  
Human Pluripotent Stem Cells ◽  
Human Beta Cell

scholarly journals Aryl hydrocarbon receptor inhibition promotes hematolymphoid development from human pluripotent stem cells

Blood ◽  
2017 ◽  
Vol 129 (26) ◽  
pp. 3428-3439 ◽  
Author(s):  
Mathew G. Angelos ◽  
Paige N. Ruh ◽  
Beau R. Webber ◽  
Robert H. Blum ◽  
Caitlin D. Ryan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Differentiation ◽  
Aryl Hydrocarbon Receptor ◽  
Small Molecule ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Aryl Hydrocarbon ◽  
Small Molecule Inhibition ◽  
Receptor Inhibition ◽  
Key Points

Key Points Small-molecule inhibition and CRISPR/Cas9 deletion of AHR promote early hematoendothelial cell differentiation from hESCs. AHR inhibition enhances the differentiation of cNK cells from hESCs whereas AHR hyperactivation supports development of ILC3s.

scholarly journals miR-212/132-Enriched Extracellular Vesicles Promote Differentiation of Induced Pluripotent Stem Cells Into Pancreatic Beta Cells

2021 ◽  
Vol 9 ◽  
Author(s):  
Chunyu Bai ◽  
Qiwei Ren ◽  
Haifeng Liu ◽  
Xiangchen Li ◽  
Weijun Guan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Beta Cell ◽  
Induced Pluripotent Stem Cells ◽  
Beta Cells ◽  
Extracellular Vesicles ◽  
Pluripotent Stem Cells ◽  
Pancreatic Beta Cells ◽  
Pancreatic Beta Cell ◽  
Induced Pluripotent

Pancreatic beta cell transplantation is the ideal method for treatment of type 1 diabetes mellitus (T1DM), and the generation of beta cells from induced pluripotent stem cells (iPSCs) of patients is a promising strategy. In this study, we improved a previous strategy to produce beta cells using extracellular vesicles (EVs) derived from mature beta cells and differentiated beta cells from iPSCs (i-Beta cells), which secreted insulin under glucose stimulation in vitro and ameliorated hyperglycemia in vivo. Mechanistic analyses revealed that EV-carried microRNA (miR)-212/132 (EV-miR-212/132) directly bound to the 3′ UTR of FBW7 to prevent its translation and FBW7 combined with NGN3 to accelerate its proteasomal degradation. EV-miR-212/132 stabilized NGN3 expression to promote differentiation of endocrine cells from induced iPSCs. Moreover, NGN3 bound to PDX1 to enhance transcription of endogenous miR-212/132 and formed a positive regulatory circuit that maintained the functions of mature pancreatic beta cells.ConclusionThis study describes a novel approach for beta cell production and supports the use of iPSCs for cell replacement therapy of T1DM.

Sign in / Sign up

Export Citation Format

Share Document