Measurement of Very Fast Exchange Rates of Individual Amide Protons in Proteins by NMR Spectroscopy

ChemPhysChem ◽  
2018 ◽  
Vol 20 (2) ◽  
pp. 231-235 ◽  
Author(s):  
Rupashree Dass ◽  
Enrico Corlianò ◽  
Frans A. A. Mulder
Keyword(s):  
Nmr Spectroscopy ◽  
Exchange Rates ◽  
Fast Exchange ◽  
Amide Protons

scholarly journals Anion modulation of the1H/2H exchange rates in backbone amide protons monitored by NMR spectroscopy

2007 ◽  
Vol 16 (12) ◽  
pp. 2733-2740 ◽  
Author(s):  
Xavier Tadeo ◽  
David Castaño ◽  
Oscar Millet
Keyword(s):  
Nmr Spectroscopy ◽  
Exchange Rates ◽  
Backbone Amide ◽  
Amide Protons

ChemInform Abstract: Exchange Rates of the Amide Protons of d-Biotin Sulfoxide Stereoisomers

ChemInform ◽  
1987 ◽  
Vol 18 (32) ◽  
Author(s):  
T. L. FOX ◽  
P. A. TIPTON ◽  
W. W. CLELAND ◽  
A. S. MILDVAN
Keyword(s):  
Exchange Rates ◽  
Abstract Exchange ◽  
Amide Protons

scholarly journals Direct and Indirect Models for Protein Chemical Denaturation are Characteristic of Opposite Dynamic Properties

2016 ◽  
Author(s):  
Liangzhong Lim ◽  
Linlin Miao ◽  
Jianxing Song
Keyword(s):  
Quantitative Analysis ◽  
Exchange Rates ◽  
Dynamic Properties ◽  
Conformational State ◽  
Backbone Dynamics ◽  
Dependent Manner ◽  
Chemical Denaturation ◽  
Concentration Dependent Manner ◽  
Denaturant Concentration ◽  
Amide Protons

AbstractTwo major models, namely direct and indirect models, have been proposed for the protein chemical denaturation but it remains challenging to experimentally demonstrate and distinguish between them. Here, by use of CD and NMR spectroscopy, we succeeded in differentiating the effects on a small but well-folded protein WW4, of GdmCl and NaSCN at diluted concentrations (≥200 mM). Both denaturants up to 200 mM have no alternation of its average structure but do reduce its thermodynamic stability to different degrees. Despite acting as the stronger denaturant, GdmCl only weakly interacts with amide protons, while NaSCN shows extensive interactions with both hydrophobic side chains and amide protons. Although both denaturants show no significant perturbation on overall ps-ns backbone dynamics of WW4, GdmCl suppresses while NaSCN enhances its μs-ms backbone dynamics in a denaturant concentration dependent manner. Quantitative analysis reveals that although they dramatically raise exchange rates, GdmCl slightly increases while NaSCN reduces the population of the major conformational state. Our study represents the first report deciphering that GdmCl and NaSCN appear to destabilize a protein following two models respectively, which are characteristic of opposite μs-ms dynamics.

ESR-Untersuchungen von Nitroxyl-Radikalen / ESR Studies of Nitroxyl Radicals

1976 ◽  
Vol 31 (9) ◽  
pp. 1265-1269 ◽  
Author(s):  
K. Scheffler ◽  
P. Schuler ◽  
G. Sosnovsky
Keyword(s):  
Exchange Rates ◽  
Exchange Interaction ◽  
Esr Spectroscopy ◽  
Esr Spectra ◽  
Nitroxyl Radicals ◽  
Radical Species ◽  
Fast Exchange ◽  
Theoretical Line ◽  
Multispin Systems ◽  
Line Positions

Mono-, di- and trinitroxylradicals are investigated in solution by ESR spectroscopy. The ESR spectra of all multispin systems studied can be interpreted under the assumption of an exchange interaction of the radical electrons. The exchange energies J are evaluated by comparison of experimental and theoretical line positions. The temperature dependence of J leads to activation energies around 1.5 Kcal/Mol. The analysis of proton hfs of the biradicals indicates the existence of two radical species which slow and fast exchange rates.

Deconvolution of fast exchange equilibrium states in NMR spectroscopy using virtual reference standards and probability theory

2020 ◽  
Vol 18 (35) ◽  
pp. 6927-6934
Author(s):  
Xiaozhou Ji ◽  
Nina C. Gonnella ◽  
Dongyue Xin
Keyword(s):  
Probability Theory ◽  
Nmr Spectroscopy ◽  
Reference Standards ◽  
Equilibrium States ◽  
Accurate Prediction ◽  
Exchange Equilibrium ◽  
Virtual Reference ◽  
Fast Exchange ◽  
Giao Calculations

DFT–GIAO calculations combined with probability theory enable accurate prediction of fast exchange equilibrium states based on one experimental NMR dataset.

NMR Study of Miscibility in Polyepichlorohydrin/Poly(Vinylmethylether) Blends

1994 ◽  
Vol 67 (4) ◽  
pp. 629-635 ◽  
Author(s):  
K. J. McGrath ◽  
C. M. Roland
Keyword(s):  
Glass Transition ◽  
Nmr Spectroscopy ◽  
Chemical Shift ◽  
Glass Transition Temperatures ◽  
129Xe Nmr ◽  
Nmr Study ◽  
Miscible Blends ◽  
Fast Exchange ◽  
Exchange Regime ◽  
Fast Exchange Regime

Abstract 129Xe NMR spectroscopy is employed to demonstrate that polyepichlorohydrin and poly(vinylmethylether), which have virtually equal glass transition temperatures, form thermodynamically miscible blends. This conclusion is drawn from the appearance of a single resonance in the blend spectrum, at a chemical shift differing from that calculated for the fast exchange regime. 13C NMR results are consistent with thermodynamic miscibility, and reflect the similarity of the component dynamics.

Measurement of intrinsic exchange rates of amide protons in a 15N-labeled peptide

1995 ◽  
Vol 6 (3) ◽  
Author(s):  
Shohei Koide ◽  
Wolfgang Jahnke ◽  
PeterE. Wright
Keyword(s):  
Exchange Rates ◽  
Amide Protons
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