How Chi Sequence Modifies RecBCD Single-Stranded DNA Translocase Activity

ChemPhysChem ◽  
2017 ◽  
Vol 19 (2) ◽  
pp. 243-247 ◽  
Author(s):  
Chia-Chuan Cho ◽  
Cinya Chung ◽  
Hung-Wen Li
Keyword(s):  
Single Stranded Dna ◽  
Chi Sequence ◽  
Dna Translocase

scholarly journals Bacillus stearothermophilus PcrA Monomer Is a Single-stranded DNA Translocase but Not a Processive Helicase in Vitro

2007 ◽  
Vol 282 (37) ◽  
pp. 27076-27085 ◽  
Author(s):  
Anita Niedziela-Majka ◽  
Marla A. Chesnik ◽  
Eric J. Tomko ◽  
Timothy M. Lohman
Keyword(s):  
Bacillus Stearothermophilus ◽  
Single Stranded Dna ◽  
Dna Translocase

scholarly journals Natural transformation protein ComFA exhibits single-stranded DNA translocase activity

2021 ◽  
Author(s):  
Hannah Reed Foster ◽  
Xiaoxuan Lin ◽  
Sriram Srikant ◽  
Rachel R Cueny ◽  
Tanya G Falbel ◽  
...  
Keyword(s):  
Atp Hydrolysis ◽  
Natural Transformation ◽  
Dna Unwinding ◽  
Dna Uptake ◽  
Single Stranded Dna ◽  
Bacterial Populations ◽  
Gram Positive Bacteria ◽  
Dead Box ◽  
Outstanding Question ◽  
Dna Translocase

Natural transformation is one of the major mechanisms of horizontal gene transfer in bacterial populations and has been demonstrated in numerous species of bacteria. Despite the prevalence of natural transformation, much of the molecular mechanism remains unexplored. One major outstanding question is how the cell powers DNA import, which is rapid and highly processive. ComFA is one of a handful of proteins required for natural transformation in gram-positive bacteria. Its structural resemblance to the DEAD-box helicase family has led to a long-held hypothesis that ComFA acts as a motor to help drive DNA import into the cytosol. Here, we explored the helicase and translocase activity of ComFA to address this hypothesis. We followed the DNA-dependent ATPase activity of ComFA and, combined with mathematical modeling, demonstrated that ComFA likely translocates on single-stranded DNA from 5′ to 3′. However, this translocase activity does not lead to DNA unwinding in the conditions we tested. Further, we analyzed the ATPase cycle of ComFA and found that ATP hydrolysis stimulates the release of DNA, providing a potential mechanism for translocation. These findings help define the molecular contribution of ComFA to natural transformation and support the conclusion that ComFA plays a key role in powering DNA uptake.

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