scholarly journals Cover Picture: Electron Transfer between Genetically Modified Hansenula polymorpha Yeast Cells and Electrode Surfaces via Os-complex modified Redox Polymers (ChemPhysChem 4/2011)

ChemPhysChem ◽  
2011 ◽  
Vol 12 (4) ◽  
pp. 721-721
Author(s):  
Halyna Shkil ◽  
Albert Schulte ◽  
Dmitrii A. Guschin ◽  
Wolfgang Schuhmann
Keyword(s):  
Electron Transfer ◽  
Genetically Modified ◽  
Hansenula Polymorpha ◽  
Yeast Cells ◽  
Redox Polymers ◽  
Electrode Surfaces

Bioelectrochemical detection of L-lactate respiration using genetically modified Hansenula polymorpha yeast cells overexpressing flavocytochrome b2

2009 ◽  
Vol 76 (1-2) ◽  
pp. 175-179 ◽  
Author(s):  
Halyna Shkil ◽  
Leonard Stoica ◽  
Kostyantyn Dmytruk ◽  
Oleh Smutok ◽  
Mykhailo Gonchar ◽  
...  
Keyword(s):  
Genetically Modified ◽  
Hansenula Polymorpha ◽  
Yeast Cells ◽  
Flavocytochrome B2

scholarly journals Formaldehyde Oxidizing Enzymes and Genetically Modified Yeast Hansenula polymorpha Cells in Monitoring and Removal of Formaldehyde

10.5772/16148 ◽  
2011 ◽  
Author(s):  
Vladimir Sibirny ◽  
Olha Demkiv ◽  
Sasi Sigawi ◽  
Solomiya Paryzhak ◽  
Halyna Klepach ◽  
...  
Keyword(s):  
Genetically Modified ◽  
Hansenula Polymorpha

Effect of Metal Modification to Carbon Paper Anodes on the Performance of Yeast-Based Microbial Fuel Cells Part Ι: In the Case without Exogenous Mediator

2013 ◽  
Vol 534 ◽  
pp. 76-81 ◽  
Author(s):  
Enas Taha Kasem ◽  
Takuya Tsujiguchi ◽  
Nobuyoshi Nakagawa
Keyword(s):  
Electron Transfer ◽  
Fuel Cells ◽  
Fuel Cell ◽  
Thin Layer ◽  
Electrode Surface ◽  
Microbial Fuel Cells ◽  
Yeast Cells ◽  
Carbon Paper ◽  
Electrode Potentials ◽  
Different Thickness

Effect of modification of carbon paper with a thin layer of cobalt or gold on the performance of yeast-based microbial fuel cells was investigated. The modification was conducted by depositing Co or Au thin layer with different thickness, 5 nm and 30 nm, using a sputtering technique. The electrode performance was evaluated by measuring the electrode potentials and the fuel cell power output. The Co modification significantly increased the performance of the fuel cell, while the Au modification inhibited the performance. SEM observation indicated that the adhesion density of the yeast cells on the electrode surface was affected by the metals. It was confirmed that the electron transfer took place through the surface confined species at the mediatorless anode.

scholarly journals Peroxisome Maintenance Depends on De Novo Peroxisome Formation in Yeast Mutants Defective in Peroxisome Fission and Inheritance

2019 ◽  
Vol 20 (16) ◽  
pp. 4023 ◽  
Author(s):  
Justyna P. Wróblewska ◽  
Ida J. van der Klei
Keyword(s):  
De Novo ◽  
Hansenula Polymorpha ◽  
Yeast Cells ◽  
Wild Type ◽  
Double Deletion ◽  
Mother Cells ◽  
Yeast Mutants ◽  
Rescue Mechanism ◽  
Wild Type Yeast ◽  
In Cells

There is an ongoing debate on how peroxisomes form: by growth and fission of pre-existing peroxisomes or de novo from another membrane. It has been proposed that, in wild type yeast cells, peroxisome fission and careful segregation of the organelles over mother cells and buds is essential for organelle maintenance. Using live cell imaging we observed that cells of the yeast Hansenula polymorpha, lacking the peroxisome fission protein Pex11, still show peroxisome fission and inheritance. Also, in cells of mutants without the peroxisome inheritance protein Inp2 peroxisome segregation can still occur. In contrast, peroxisome fission and inheritance were not observed in cells of a pex11 inp2 double deletion strain. In buds of cells of this double mutant, new organelles likely appear de novo. Growth of pex11 inp2 cells on methanol, a growth substrate that requires functional peroxisomes, is retarded relative to the wild type control. Based on these observations we conclude that in H. polymorpha de novo peroxisome formation is a rescue mechanism, which is less efficient than organelle fission and inheritance to maintain functional peroxisomes.

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